PHCA_THEVB
ID PHCA_THEVB Reviewed; 162 AA.
AC P50032;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=C-phycocyanin alpha subunit;
GN Name=cpcA; OrderedLocusNames=tlr1958;
OS Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1).
OC Bacteria; Cyanobacteria; Pseudanabaenales; Thermosynechococcaceae;
OC Thermosynechococcus.
OX NCBI_TaxID=197221;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Shimazu T., Soga M., Hirano M., Katoh S.;
RT "Cloning and sequencing of the phycocyanin operon from the thermophilic
RT cyanobacterium Synechococcus elongatus.";
RL Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=12240834; DOI=10.1093/dnares/9.4.123;
RA Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S.,
RA Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C.,
RA Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takeuchi C., Yamada M., Tabata S.;
RT "Complete genome structure of the thermophilic cyanobacterium
RT Thermosynechococcus elongatus BP-1.";
RL DNA Res. 9:123-130(2002).
RN [3] {ECO:0007744|PDB:1KTP}
RP X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RX PubMed=12460674; DOI=10.1016/s0005-2728(02)00359-6;
RA Adir N., Vainer R., Lerner N.;
RT "Refined structure of c-phycocyanin from the cyanobacterium Synechococcus
RT vulcanus at 1.6 A: insights into the role of solvent molecules in thermal
RT stability and co-factor structure.";
RL Biochim. Biophys. Acta 1556:168-174(2002).
RN [4] {ECO:0007744|PDB:1JBO}
RP X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=12615541; DOI=10.1016/s1047-8477(02)00609-3;
RA Nield J., Rizkallah P.J., Barber J., Chayen N.E.;
RT "The 1.45 A three-dimensional structure of C-phycocyanin from the
RT thermophilic cyanobacterium Synechococcus elongatus.";
RL J. Struct. Biol. 141:149-155(2003).
RN [5] {ECO:0007744|PDB:3L0F}
RP X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RA Fromme R., Brune D., Fromme P.;
RT "The 3 dimensional structure of C-Phycocyanin at 1.35 A resolution.";
RL Submitted (DEC-2009) to the PDB data bank.
RN [6] {ECO:0007744|PDB:4Q70}
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RA Fromme R., Roy-Chowdhury S., Basu S., Yoon C., Brune D., Fromme P.;
RT "Observations on the Light Harvesting Protein Phycocyanin structure in high
RT resolution using a femtosecond X-Ray laser.";
RL Submitted (APR-2014) to the PDB data bank.
RN [7] {ECO:0007744|PDB:4Z8K}
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=26177184; DOI=10.1107/s2052252515009811;
RA Conrad C.E., Basu S., James D., Wang D., Schaffer A., Roy-Chowdhury S.,
RA Zatsepin N.A., Aquila A., Coe J., Gati C., Hunter M.S., Koglin J.E.,
RA Kupitz C., Nelson G., Subramanian G., White T.A., Zhao Y., Zook J.,
RA Boutet S., Cherezov V., Spence J.C., Fromme R., Weierstall U., Fromme P.;
RT "A novel inert crystal delivery medium for serial femtosecond
RT crystallography.";
RL IUCrJ 2:421-430(2015).
RN [8] {ECO:0007744|PDB:4ZIZ}
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=26306196; DOI=10.1107/s2052252515013160;
RA Fromme R., Ishchenko A., Metz M., Chowdhury S.R., Basu S., Boutet S.,
RA Fromme P., White T.A., Barty A., Spence J.C., Weierstall U., Liu W.,
RA Cherezov V.;
RT "Serial femtosecond crystallography of soluble proteins in lipidic cubic
RT phase.";
RL IUCrJ 2:545-551(2015).
RN [9] {ECO:0007744|PDB:5UVK}
RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=28875031; DOI=10.1107/s205225251700570x;
RA Martin-Garcia J.M., Conrad C.E., Nelson G., Stander N., Zatsepin N.A.,
RA Zook J., Zhu L., Geiger J., Chun E., Kissick D., Hilgart M.C., Ogata C.,
RA Ishchenko A., Nagaratnam N., Roy-Chowdhury S., Coe J., Subramanian G.,
RA Schaffer A., James D., Ketwala G., Venugopalan N., Xu S., Corcoran S.,
RA Ferguson D., Weierstall U., Spence J.C.H., Cherezov V., Fromme P.,
RA Fischetti R.F., Liu W.;
RT "Serial millisecond crystallography of membrane and soluble protein
RT microcrystals using synchrotron radiation.";
RL IUCrJ 4:439-454(2017).
RN [10] {ECO:0007744|PDB:5MJM, ECO:0007744|PDB:5MJP, ECO:0007744|PDB:5MJQ, ECO:0007744|PDB:5O7M}
RP X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=29097720; DOI=10.1038/s41467-017-01417-3;
RA Meents A., Wiedorn M.O., Srajer V., Henning R., Sarrou I., Bergtholdt J.,
RA Barthelmess M., Reinke P.Y.A., Dierksmeyer D., Tolstikova A., Schaible S.,
RA Messerschmidt M., Ogata C.M., Kissick D.J., Taft M.H., Manstein D.J.,
RA Lieske J., Oberthuer D., Fischetti R.F., Chapman H.N.;
RT "Pink-beam serial crystallography.";
RL Nat. Commun. 8:1281-1281(2017).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit, which further
CC assembles into trimers (PubMed:12460674, PubMed:12615541). The trimers
CC assemble into hexamers (Probable). {ECO:0000269|PubMed:12460674,
CC ECO:0000269|PubMed:12615541, ECO:0000305, ECO:0007744|PDB:1JBO,
CC ECO:0007744|PDB:1KTP, ECO:0007744|PDB:3L0F, ECO:0007744|PDB:4Q70,
CC ECO:0007744|PDB:4Z8K, ECO:0007744|PDB:4ZIZ, ECO:0007744|PDB:5UVK}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:26306196}; Peripheral membrane protein
CC {ECO:0000269|PubMed:26306196}; Cytoplasmic side {ECO:0000250}.
CC Note=Part of the phycobilisome rod. {ECO:0000269|PubMed:12615541,
CC ECO:0000269|PubMed:26306196}.
CC -!- PTM: Contains one covalently linked phycocyanobilin chromophore.
CC {ECO:0000269|PubMed:12460674, ECO:0000269|PubMed:12615541,
CC ECO:0000269|PubMed:26177184, ECO:0000269|PubMed:26306196,
CC ECO:0000269|PubMed:28875031, ECO:0007744|PDB:1JBO,
CC ECO:0007744|PDB:1KTP, ECO:0007744|PDB:3L0F, ECO:0007744|PDB:4Q70,
CC ECO:0007744|PDB:4Z8K, ECO:0007744|PDB:4ZIZ, ECO:0007744|PDB:5UVK}.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; D13173; BAA02456.1; -; Genomic_DNA.
DR EMBL; BA000039; BAC09510.1; -; Genomic_DNA.
DR RefSeq; NP_682748.1; NC_004113.1.
DR RefSeq; WP_011057793.1; NC_004113.1.
DR PDB; 1JBO; X-ray; 1.45 A; A=1-162.
DR PDB; 1KTP; X-ray; 1.60 A; A=1-162.
DR PDB; 3L0F; X-ray; 1.35 A; A=1-162.
DR PDB; 4Q70; X-ray; 1.85 A; A=1-162.
DR PDB; 4Z8K; X-ray; 2.50 A; A=1-162.
DR PDB; 4ZIZ; X-ray; 1.75 A; A=1-162.
DR PDB; 5MJM; X-ray; 2.30 A; A=1-162.
DR PDB; 5MJP; X-ray; 2.11 A; A=1-162.
DR PDB; 5MJQ; X-ray; 2.70 A; A=1-162.
DR PDB; 5O7M; X-ray; 2.46 A; A=1-162.
DR PDB; 5UVK; X-ray; 3.10 A; A=1-162.
DR PDB; 6YPQ; X-ray; 1.29 A; A=1-162.
DR PDB; 6YQ8; X-ray; 1.82 A; A=1-162.
DR PDB; 6YQG; X-ray; 1.45 A; A=1-162.
DR PDB; 6YYJ; X-ray; 2.16 A; A/C/E=1-162.
DR PDB; 7S50; X-ray; 2.10 A; A=1-162.
DR PDBsum; 1JBO; -.
DR PDBsum; 1KTP; -.
DR PDBsum; 3L0F; -.
DR PDBsum; 4Q70; -.
DR PDBsum; 4Z8K; -.
DR PDBsum; 4ZIZ; -.
DR PDBsum; 5MJM; -.
DR PDBsum; 5MJP; -.
DR PDBsum; 5MJQ; -.
DR PDBsum; 5O7M; -.
DR PDBsum; 5UVK; -.
DR PDBsum; 6YPQ; -.
DR PDBsum; 6YQ8; -.
DR PDBsum; 6YQG; -.
DR PDBsum; 6YYJ; -.
DR PDBsum; 7S50; -.
DR AlphaFoldDB; P50032; -.
DR SMR; P50032; -.
DR MINT; P50032; -.
DR STRING; 197221.22295684; -.
DR EnsemblBacteria; BAC09510; BAC09510; BAC09510.
DR KEGG; tel:tlr1958; -.
DR PATRIC; fig|197221.4.peg.2048; -.
DR eggNOG; ENOG502Z85C; Bacteria.
DR OMA; TTQMQGP; -.
DR OrthoDB; 1513296at2; -.
DR EvolutionaryTrace; P50032; -.
DR Proteomes; UP000000440; Chromosome.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006246; Phycocyanin_a.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01338; phycocy_alpha; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chromophore;
KW Electron transport; Membrane; Photosynthesis; Phycobilisome;
KW Reference proteome; Thylakoid; Transport.
FT CHAIN 1..162
FT /note="C-phycocyanin alpha subunit"
FT /id="PRO_0000199128"
FT BINDING 84
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0007744|PDB:1JBO, ECO:0007744|PDB:1KTP,
FT ECO:0007744|PDB:3L0F, ECO:0007744|PDB:4Q70,
FT ECO:0007744|PDB:4Z8K, ECO:0007744|PDB:4ZIZ,
FT ECO:0007744|PDB:5MJM, ECO:0007744|PDB:5MJP,
FT ECO:0007744|PDB:5MJQ, ECO:0007744|PDB:5O7M,
FT ECO:0007744|PDB:5UVK"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 21..46
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 48..62
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:6YPQ"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 78..101
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 105..110
FT /evidence="ECO:0007829|PDB:6YPQ"
FT TURN 111..114
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 115..122
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 126..139
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 144..160
FT /evidence="ECO:0007829|PDB:6YPQ"
SQ SEQUENCE 162 AA; 17443 MW; C0043A90C93CFDD5 CRC64;
MKTPITEAIA AADTQGRFLS NTELQAVDGR FKRAVASMEA ARALTNNAQS LIDGAAQAVY
QKFPYTTTMQ GSQYASTPEG KAKCARDIGY YLRMVTYCLV AGGTGPMDEY LIAGLSEINS
TFDLSPSWYI EALKYIKANH GLTGQAAVEA NAYIDYAINA LS
