PHCB1_SYNP6
ID PHCB1_SYNP6 Reviewed; 173 AA.
AC P00312; P11391; Q79DN9;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=C-phycocyanin-1 beta subunit;
GN Name=cpcB1; Synonyms=cpcB; OrderedLocusNames=syc0496_c;
OS Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis
OS nidulans).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=269084;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7524727; DOI=10.1007/bf00039542;
RA Bhalerao R.P., Lind L.K., Gustafsson P.;
RT "Cloning of the cpcE and cpcF genes from Synechococcus sp. PCC 6301 and
RT their inactivation in Synechococcus sp. PCC 7942.";
RL Plant Mol. Biol. 26:313-326(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27144 / PCC 6301 / SAUG 1402/1;
RX PubMed=17211581; DOI=10.1007/s11120-006-9122-4;
RA Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M.,
RA Kanehisa M., Omata T., Sugiura M., Sugita M.;
RT "Complete nucleotide sequence of the freshwater unicellular cyanobacterium
RT Synechococcus elongatus PCC 6301 chromosome: gene content and
RT organization.";
RL Photosyn. Res. 93:55-67(2007).
RN [3]
RP PROTEIN SEQUENCE OF 2-173, AND CHROMOPHORE ATTACHMENT.
RC STRAIN=ATCC 27144 / PCC 6301 / SAUG 1402/1;
RX PubMed=412848; DOI=10.1016/s0021-9258(17)38290-x;
RA Freidenreich P., Apell G.S., Glazer A.N.;
RT "Structural studies on phycobiliproteins II. C-phycocyanin: amino acid
RT sequence of the beta subunit. Specific cleavage of the alpha subunit.";
RL J. Biol. Chem. 253:212-219(1978).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 52-78.
RA Lind L.K., Kalla S.R., Loenneborg A., Oequist G., Gustafsson P.;
RT "Cloning of the beta-phycocyanin gene from Anacystis nidulans.";
RL FEBS Lett. 188:27-32(1985).
RN [5]
RP PROTEIN SEQUENCE OF 81-87 AND 136-173, SUBUNIT, AND CHROMOPHORE ATTACHMENT.
RC STRAIN=ATCC 27144 / PCC 6301 / SAUG 1402/1;
RX PubMed=412846; DOI=10.1016/s0021-9258(17)38289-3;
RA Williams V.P., Glazer A.N.;
RT "Structural studies on phycobiliproteins. I. Bilin-containing peptides of
RT C-phycocyanin.";
RL J. Biol. Chem. 253:202-211(1978).
RN [6]
RP METHYLATION AT ASN-73.
RX PubMed=2447072; DOI=10.1016/s0021-9258(18)45384-7;
RA Klotz A.V., Glazer A.N.;
RT "Gamma-N-methylasparagine in phycobiliproteins. Occurrence, location, and
RT biosynthesis.";
RL J. Biol. Chem. 262:17350-17355(1987).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit (PubMed:412846).
CC The heterodimer further assembles into trimers and the trimers into
CC hexamers (By similarity). {ECO:0000250|UniProtKB:P06539,
CC ECO:0000269|PubMed:412846}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane; Peripheral membrane
CC protein; Cytoplasmic side. Note=Part of the phycobilisome rod.
CC -!- PTM: Contains two covalently linked bilin chromophores.
CC {ECO:0000269|PubMed:412846, ECO:0000269|PubMed:412848}.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; M94218; AAA64526.1; -; Genomic_DNA.
DR EMBL; AP008231; BAD78686.1; -; Genomic_DNA.
DR RefSeq; WP_011242808.1; NC_006576.1.
DR AlphaFoldDB; P00312; -.
DR SMR; P00312; -.
DR STRING; 269084.syc0496_c; -.
DR iPTMnet; P00312; -.
DR EnsemblBacteria; BAD78686; BAD78686; syc0496_c.
DR KEGG; syc:syc0496_c; -.
DR eggNOG; ENOG502Z7NE; Bacteria.
DR OMA; GITPGDC; -.
DR Proteomes; UP000001175; Chromosome.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006247; Phycocyanin_b.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01339; phycocy_beta; 1.
PE 1: Evidence at protein level;
KW Antenna complex; Bile pigment; Chromophore; Direct protein sequencing;
KW Electron transport; Membrane; Methylation; Photosynthesis; Phycobilisome;
KW Thylakoid; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:412848"
FT CHAIN 2..173
FT /note="C-phycocyanin-1 beta subunit"
FT /id="PRO_0000199158"
FT BINDING 83
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="1"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:412846,
FT ECO:0000269|PubMed:412848"
FT BINDING 154
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="2"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:412846,
FT ECO:0000269|PubMed:412848"
FT MOD_RES 73
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000269|PubMed:2447072"
FT CONFLICT 28..29
FT /note="LS -> SL (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="Missing (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="N -> D (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="L -> S (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 127
FT /note="S -> L (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="Missing (in Ref. 3; AA sequence and 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 173 AA; 18270 MW; 416A6895F83B0A89 CRC64;
MTFDAFTKVV AQADARGEFL SDAQLDALSR LVAEGNKRID TVNRITGNAS SIVANAARAL
FAEQPSLIAP GGNAYTNRRM AACLRDMEII LRYVTYAVFT GDASILDDRC LNGLRETYLA
LGVPGASVAE GVRKMKDAAV AIVSDRNGIT QGDCSAIISE LGSYFDKAAA AVA
