ID   PHCB_APHFL              Reviewed;         129 AA.
AC   P85869;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=C-phycocyanin beta subunit {ECO:0000250|UniProtKB:P00310};
DE   Flags: Fragments;
GN   Name=cpcB {ECO:0000250|UniProtKB:P00310};
OS   Aphanizomenon flos-aquae.
OC   Bacteria; Cyanobacteria; Nostocales; Aphanizomenonaceae; Aphanizomenon.
OX   NCBI_TaxID=1176;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RX   PubMed=19053161; DOI=10.1002/jms.1526;
RA   Rinalducci S., Roepstorff P., Zolla L.;
RT   "De novo sequence analysis and intact mass measurements for
RT   characterization of phycocyanin subunit isoforms from the blue-green alga
RT   Aphanizomenon flos-aquae.";
RL   J. Mass Spectrom. 44:503-515(2009).
CC   -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC       phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC       phycobiliprotein in the PBS rod.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta subunit, which further
CC       assembles into trimers and the trimers into hexamers.
CC       {ECO:0000250|UniProtKB:P06539}.
CC   -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC       {ECO:0000250|UniProtKB:P00310}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:P00310}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:P00310}. Note=Part of the phycobilisome rod.
CC       {ECO:0000250|UniProtKB:P00310}.
CC   -!- PTM: Two isomers exist. {ECO:0000269|PubMed:19053161}.
CC   -!- PTM: Contains two covalently linked bilin chromophores.
CC       {ECO:0000250|UniProtKB:P06539}.
CC   -!- MASS SPECTROMETRY: Mass=19220.69; Method=MALDI; Note=Major isomer.;
CC       Evidence={ECO:0000269|PubMed:19053161};
CC   -!- MASS SPECTROMETRY: Mass=19391.05; Method=MALDI; Note=Minor isomer.;
CC       Evidence={ECO:0000269|PubMed:19053161};
CC   -!- MASS SPECTROMETRY: Mass=19221.93; Method=Electrospray; Note=Major
CC       isomer.; Evidence={ECO:0000269|PubMed:19053161};
CC   -!- MASS SPECTROMETRY: Mass=19384.78; Method=Electrospray; Note=Minor
CC       isomer.; Evidence={ECO:0000269|PubMed:19053161};
CC   -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000255}.
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DR   AlphaFoldDB; P85869; -.
DR   SMR; P85869; -.
DR   GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR   GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.490.20; -; 1.
DR   InterPro; IPR009050; Globin-like_sf.
DR   InterPro; IPR012128; Phycobilisome_asu/bsu.
DR   InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR   Pfam; PF00502; Phycobilisome; 1.
DR   PIRSF; PIRSF000081; Phycocyanin; 1.
DR   SUPFAM; SSF46458; SSF46458; 1.
PE   1: Evidence at protein level;
KW   Antenna complex; Bile pigment; Chromophore; Direct protein sequencing;
KW   Electron transport; Membrane; Methylation; Photosynthesis; Phycobilisome;
KW   Thylakoid; Transport.
FT   CHAIN           <1..>129
FT                   /note="C-phycocyanin beta subunit"
FT                   /id="PRO_0000371458"
FT   BINDING         116
FT                   /ligand="(2R,3E)-phycocyanobilin"
FT                   /ligand_id="ChEBI:CHEBI:85275"
FT                   /ligand_label="2"
FT                   /note="covalent, via 1 link"
FT                   /evidence="ECO:0000250|UniProtKB:P00310"
FT   MOD_RES         62
FT                   /note="N4-methylasparagine"
FT                   /evidence="ECO:0000250|UniProtKB:P00310"
FT   NON_CONS        25..26
FT                   /evidence="ECO:0000303|PubMed:19053161"
FT   NON_CONS        68..69
FT                   /evidence="ECO:0000303|PubMed:19053161"
FT   NON_CONS        93..94
FT                   /evidence="ECO:0000303|PubMed:19053161"
FT   NON_CONS        113..114
FT                   /evidence="ECO:0000303|PubMed:19053161"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:19053161"
FT   NON_TER         129
FT                   /evidence="ECO:0000303|PubMed:19053161"
SQ   SEQUENCE   129 AA;  13769 MW;  CADF91FA093D7D9C CRC64;
     VVSQADARGE FLSTEQLDAL TAVVARRLDV VNRITSNASA IVTNAARSLF EEQPNLIAPG
     GNAYTNRRRD MEIILRYVTY AAIAGDASVL DDRRETYQAL GTPGASVAVG VGKGDCSSLV
     SEVASYFDR