PHCB_NOSS1
ID PHCB_NOSS1 Reviewed; 173 AA.
AC P07120; Q9F460;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=C-phycocyanin beta subunit;
GN Name=cpcB; OrderedLocusNames=alr0528;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3109890; DOI=10.1002/j.1460-2075.1987.tb04833.x;
RA Belknap W.R., Haselkorn R.;
RT "Cloning and light regulation of expression of the phycocyanin operon of
RT the cyanobacterium Anabaena.";
RL EMBO J. 6:871-884(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11237320; DOI=10.1006/abio.2000.4979;
RA Cai Y.A., Murphy J.T., Wedemayer G.J., Glazer A.N.;
RT "Recombinant phycobiliproteins. Recombinant C-phycocyanins equipped with
RT affinity tags, oligomerization, and biospecific recognition domains.";
RL Anal. Biochem. 290:186-204(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
RN [4]
RP CHROMOPHORE ATTACHMENT.
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=16452471; DOI=10.1074/jbc.m513796200;
RA Zhao K.H., Su P., Li J., Tu J.M., Zhou M., Bubenzer C., Scheer H.;
RT "Chromophore attachment to phycobiliprotein beta-subunits:
RT phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-
RT like protein from Anabaena Sp. PCC7120.";
RL J. Biol. Chem. 281:8573-8581(2006).
RN [5]
RP PROTEIN SEQUENCE OF 2-21, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=24550276; DOI=10.1073/pnas.1320599111;
RA Watanabe M., Semchonok D.A., Webber-Birungi M.T., Ehira S., Kondo K.,
RA Narikawa R., Ohmori M., Boekema E.J., Ikeuchi M.;
RT "Attachment of phycobilisomes in an antenna-photosystem I supercomplex of
RT cyanobacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:2512-2517(2014).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit. Part of 2 PBS rod
CC complexes, the conventional PBS rod and a photosystem I-specific CpcL-
CC PBS rod (PubMed:24550276). {ECO:0000250, ECO:0000269|PubMed:24550276}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:24550276}; Peripheral membrane protein
CC {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Part of the
CC phycobilisome rod. {ECO:0000250}.
CC -!- PTM: Contains two covalently linked bilin chromophores. {ECO:0000250,
CC ECO:0000250|UniProtKB:P06539}.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; X05239; CAA28862.1; -; Genomic_DNA.
DR EMBL; AF178757; AAG09316.1; -; Genomic_DNA.
DR EMBL; BA000019; BAB72486.1; -; Genomic_DNA.
DR PIR; AG1872; AG1872.
DR PIR; B29674; B29674.
DR RefSeq; WP_010994704.1; NZ_RSCN01000059.1.
DR PDB; 7EYD; EM; 3.90 A; C1/C2/C3/C4/C5/C6/C7/CA/E1/E2/E3/E4/E5/E6/E7/EA/G1/G2/G3/G4/G5/G6/G7/GA/I1/I2/I3/I4/I5/I6=1-173.
DR PDBsum; 7EYD; -.
DR AlphaFoldDB; P07120; -.
DR SMR; P07120; -.
DR STRING; 103690.17129873; -.
DR EnsemblBacteria; BAB72486; BAB72486; BAB72486.
DR GeneID; 58725693; -.
DR KEGG; ana:alr0528; -.
DR eggNOG; ENOG502Z7NE; Bacteria.
DR OMA; GITPGDC; -.
DR OrthoDB; 1307812at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006247; Phycocyanin_b.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01339; phycocy_beta; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chromophore;
KW Direct protein sequencing; Electron transport; Membrane; Methylation;
KW Photosynthesis; Phycobilisome; Reference proteome; Thylakoid; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:16452471"
FT CHAIN 2..173
FT /note="C-phycocyanin beta subunit"
FT /id="PRO_0000199142"
FT BINDING 83
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="1"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000250|UniProtKB:P06539"
FT BINDING 154
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="2"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000250|UniProtKB:P06539"
FT MOD_RES 73
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000250|UniProtKB:P06539"
FT CONFLICT 163
FT /note="S -> C (in Ref. 1; CAA28862)"
FT /evidence="ECO:0000305"
FT CONFLICT 171
FT /note="Missing (in Ref. 1; CAA28862)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 173 AA; 18387 MW; 5AE5F41CA5688F26 CRC64;
MTLDVFTKVV SQADSRGEFL SNEQLDALAN VVKEGNKRLD VVNRITSNAS AIVTNAARAL
FEEQPQLIAP GGNAYTNRRM AACLRDMEII LRYVTYAILA GDASVLDDRC LNGLRETYQA
LGTPGSSVAV GVQKMKDAAV GIANDPNGIT KGDCSQLISE VASYFDRAAA AVG
