PHCB_SYNE7
ID PHCB_SYNE7 Reviewed; 173 AA.
AC P06539; Q31PD7; Q57358;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=C-phycocyanin beta subunit;
GN Name=cpcB1; Synonyms=cpcB; OrderedLocusNames=Synpcc7942_1047;
GN and
GN Name=cpcB2; OrderedLocusNames=Synpcc7942_1052;
OS Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS R2).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX NCBI_TaxID=1140;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3104880; DOI=10.1093/nar/15.5.2394;
RA Lau P.C.K., Condie A., Alvarado-Urbina G., Lau R.H.;
RT "Nucleotide sequence of phycocyanin beta-subunit gene of cyanobacterium
RT Anacystis nidulans strain R2.";
RL Nucleic Acids Res. 15:2394-2394(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2454910; DOI=10.1128/jb.170.7.2961-2970.1988;
RA Kalla R.S., Lind L.K., Lidholm J., Gustafsson P.;
RT "Transcriptional organization of the phycocyanin subunit gene clusters of
RT the cyanobacterium Anacystis nidulans UTEX 625.";
RL J. Bacteriol. 170:2961-2970(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7942 / FACHB-805;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Kyrpides N., Lykidis A., Richardson P.;
RT "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-173.
RX PubMed=3036657; DOI=10.1016/0378-1119(87)90391-x;
RA Lau R.H., Alvarado-Urbina G., Lau P.C.K.;
RT "Phycocyanin alpha-subunit gene of Anacystis nidulans R2: cloning,
RT nucleotide sequencing and expression in Escherichia coli.";
RL Gene 52:21-29(1987).
RN [5] {ECO:0007744|PDB:4H0M}
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, PROBABLE REMOVAL OF N-TERMINAL METHIONINE, SUBUNIT, AND
RP METHYLATION AT ASN-73.
RC STRAIN=PCC 7942 / FACHB-805;
RX PubMed=23201474; DOI=10.1016/j.bbabio.2012.11.006;
RA Marx A., Adir N.;
RT "Allophycocyanin and phycocyanin crystal structures reveal facets of
RT phycobilisome assembly.";
RL Biochim. Biophys. Acta 1827:311-318(2013).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit, which further
CC assembles into trimers and the trimers into hexamers.
CC {ECO:0000269|PubMed:23201474, ECO:0007744|PDB:4H0M}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Cytoplasmic side
CC {ECO:0000250}. Note=Part of the phycobilisome rod. {ECO:0000250}.
CC -!- PTM: Contains two covalently linked bilin chromophores.
CC {ECO:0000269|PubMed:23201474}.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; X04916; CAA28585.1; -; Genomic_DNA.
DR EMBL; M94218; AAA64531.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB57077.1; -; Genomic_DNA.
DR EMBL; CP000100; ABB57082.1; -; Genomic_DNA.
DR EMBL; M16325; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; M22706; AAA21760.1; -; Genomic_DNA.
DR EMBL; M22704; AAA21759.1; -; Genomic_DNA.
DR PIR; A26577; CFYCB.
DR RefSeq; WP_011242808.1; NC_007604.1.
DR PDB; 4H0M; X-ray; 2.20 A; B/D/F/H/J/L/N/P/R/T/V/X=1-173.
DR PDB; 7D6W; X-ray; 2.15 A; B/D/F/H/J/L=2-173.
DR PDBsum; 4H0M; -.
DR PDBsum; 7D6W; -.
DR AlphaFoldDB; P06539; -.
DR SMR; P06539; -.
DR STRING; 1140.Synpcc7942_1047; -.
DR PRIDE; P06539; -.
DR EnsemblBacteria; ABB57077; ABB57077; Synpcc7942_1047.
DR EnsemblBacteria; ABB57082; ABB57082; Synpcc7942_1052.
DR KEGG; syf:Synpcc7942_1047; -.
DR KEGG; syf:Synpcc7942_1052; -.
DR eggNOG; ENOG502Z7NE; Bacteria.
DR HOGENOM; CLU_104219_0_0_3; -.
DR OMA; GITPGDC; -.
DR OrthoDB; 1307812at2; -.
DR BioCyc; MetaCyc:SYNPCC7942_1047-MON; -.
DR BioCyc; SYNEL:SYNPCC7942_1047-MON; -.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006247; Phycocyanin_b.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01339; phycocy_beta; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chromophore;
KW Electron transport; Membrane; Methylation; Photosynthesis; Phycobilisome;
KW Thylakoid; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000305|PubMed:23201474"
FT CHAIN 2..173
FT /note="C-phycocyanin beta subunit"
FT /id="PRO_0000199160"
FT BINDING 83
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="1"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:23201474,
FT ECO:0007744|PDB:4H0M"
FT BINDING 154
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="2"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:23201474,
FT ECO:0007744|PDB:4H0M"
FT MOD_RES 73
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000269|PubMed:23201474,
FT ECO:0007744|PDB:4H0M"
FT HELIX 5..15
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 22..33
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 35..47
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 49..63
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 65..67
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 77..100
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 104..109
FT /evidence="ECO:0007829|PDB:4H0M"
FT TURN 110..113
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 114..121
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 125..143
FT /evidence="ECO:0007829|PDB:4H0M"
FT HELIX 155..172
FT /evidence="ECO:0007829|PDB:4H0M"
SQ SEQUENCE 173 AA; 18270 MW; 416A6895F83B0A89 CRC64;
MTFDAFTKVV AQADARGEFL SDAQLDALSR LVAEGNKRID TVNRITGNAS SIVANAARAL
FAEQPSLIAP GGNAYTNRRM AACLRDMEII LRYVTYAVFT GDASILDDRC LNGLRETYLA
LGVPGASVAE GVRKMKDAAV AIVSDRNGIT QGDCSAIISE LGSYFDKAAA AVA
