PHCB_SYNP2
ID PHCB_SYNP2 Reviewed; 172 AA.
AC P03944; B1XIT8;
DT 23-OCT-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-1986, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=C-phycocyanin subunit beta;
DE Short=beta-PC;
GN Name=cpcB; OrderedLocusNames=SYNPCC7002_A2209;
OS Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS quadruplicatum).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC unclassified Synechococcus.
OX NCBI_TaxID=32049;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=6096868; DOI=10.1073/pnas.81.24.7946;
RA de Lorimier R., Bryant D.A., Porter R.D., Liu W.-Y., Jay E.,
RA Stevens S.E. Jr.;
RT "Genes for the alpha and beta subunits of phycocyanin.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:7946-7950(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=6438628; DOI=10.1073/pnas.81.22.6983;
RA Pilot T.J., Fox J.L.;
RT "Cloning and sequencing of the genes encoding the alpha and beta subunits
RT of C-phycocyanin from the cyanobacterium Agmenellum quadruplicatum.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:6983-6987(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT "Complete sequence of Synechococcus sp. PCC 7002.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP METHYLATION AT ASN-72.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=2447072; DOI=10.1016/s0021-9258(18)45384-7;
RA Klotz A.V., Glazer A.N.;
RT "Gamma-N-methylasparagine in phycobiliproteins. Occurrence, location, and
RT biosynthesis.";
RL J. Biol. Chem. 262:17350-17355(1987).
RN [5]
RP TERTIARY STRUCTURE AROUND METHYLATED SITE.
RA Duerring M., Huber R., Bode W.;
RT "The structure of gamma-N-methylasparagine in C-phycocyanin from
RT Mastigocladus laminosus and Agmenellum quadruplicatum.";
RL FEBS Lett. 236:167-170(1988).
RN [6]
RP MASS SPECTROMETRY, AND CHROMOPHORE ATTACHMENT.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=18199754; DOI=10.1074/jbc.m708164200;
RA Shen G., Schluchter W.M., Bryant D.A.;
RT "Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the
RT cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric
RT phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin
RT subunits.";
RL J. Biol. Chem. 283:7503-7512(2008).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit, which further
CC assembles into trimers and the trimers into hexamers.
CC {ECO:0000250|UniProtKB:P06539}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Cytoplasmic side
CC {ECO:0000250}. Note=Part of the phycobilisome rod. {ECO:0000250}.
CC -!- PTM: Contains two covalently linked bilin chromophores. The chromophore
CC on position 82 is added by the phycocyanobilin lyase CpcUS, while the
CC chromophore on position 153 is added by the phycocyanobilin lyase CpcT.
CC {ECO:0000269|PubMed:18199754}.
CC -!- MASS SPECTROMETRY: Mass=19522; Method=Electrospray; Note=The measured
CC mass is that of the methylated protein plus two covalently linked
CC phycocyanobilin chromophores.; Evidence={ECO:0000269|PubMed:18199754};
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; K02660; AAB05343.1; -; Genomic_DNA.
DR EMBL; K02659; AAB05341.1; -; Genomic_DNA.
DR EMBL; CP000951; ACB00190.1; -; Genomic_DNA.
DR RefSeq; WP_012307808.1; NC_010475.1.
DR PDB; 7EXT; EM; 3.50 A; C1/C2/C4/C5/C6/C8/E1/E2/E4/E5/E6/E8/G1/G2/G4/G5/G6/G8/I1/I2/I4/I5/I6/I8/K1/K2/K4/K5/K6/K8=1-172.
DR PDBsum; 7EXT; -.
DR AlphaFoldDB; P03944; -.
DR SMR; P03944; -.
DR STRING; 32049.SYNPCC7002_A2209; -.
DR iPTMnet; P03944; -.
DR PRIDE; P03944; -.
DR EnsemblBacteria; ACB00190; ACB00190; SYNPCC7002_A2209.
DR KEGG; syp:SYNPCC7002_A2209; -.
DR eggNOG; ENOG502Z7NE; Bacteria.
DR HOGENOM; CLU_104219_0_0_3; -.
DR OMA; GITPGDC; -.
DR Proteomes; UP000001688; Chromosome.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006247; Phycocyanin_b.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01339; phycocy_beta; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chromophore;
KW Electron transport; Membrane; Methylation; Photosynthesis; Phycobilisome;
KW Reference proteome; Thylakoid; Transport.
FT CHAIN 1..172
FT /note="C-phycocyanin subunit beta"
FT /id="PRO_0000199157"
FT BINDING 82
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="1"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000305|PubMed:18199754"
FT BINDING 153
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="2"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000305|PubMed:18199754"
FT MOD_RES 72
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000269|PubMed:18199754,
FT ECO:0000269|PubMed:2447072"
SQ SEQUENCE 172 AA; 18336 MW; 70D82718A77767EC CRC64;
MFDIFTRVVS QADARGEFIS SDKLEALKKV VAEGTKRSDA VSRMTNNASS IVTNAARQLF
ADQPQLIAPG GNAYTNRRMA ACLRDMEIIL RYVTYATFTG DASVLNDRCL NGLRETYVAL
GVPGASVAAG VRAMGKAAVA IVMDPAGVTS GDCSSLQQEI ELYFETAAKA VE
