PHCB_THEVB
ID PHCB_THEVB Reviewed; 172 AA.
AC P50033;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=C-phycocyanin beta subunit;
GN Name=cpcB; OrderedLocusNames=tlr1957;
OS Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1).
OC Bacteria; Cyanobacteria; Pseudanabaenales; Thermosynechococcaceae;
OC Thermosynechococcus.
OX NCBI_TaxID=197221;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Shimazu T., Soga M., Hirano M., Katoh S.;
RT "Cloning and sequencing of the phycocyanin operon from the thermophilic
RT cyanobacterium Synechococcus elongatus.";
RL Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=12240834; DOI=10.1093/dnares/9.4.123;
RA Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S.,
RA Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C.,
RA Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takeuchi C., Yamada M., Tabata S.;
RT "Complete genome structure of the thermophilic cyanobacterium
RT Thermosynechococcus elongatus BP-1.";
RL DNA Res. 9:123-130(2002).
RN [3] {ECO:0007744|PDB:1KTP}
RP X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND METHYLATION AT ASN-72.
RX PubMed=12460674; DOI=10.1016/s0005-2728(02)00359-6;
RA Adir N., Vainer R., Lerner N.;
RT "Refined structure of c-phycocyanin from the cyanobacterium Synechococcus
RT vulcanus at 1.6 A: insights into the role of solvent molecules in thermal
RT stability and co-factor structure.";
RL Biochim. Biophys. Acta 1556:168-174(2002).
RN [4] {ECO:0007744|PDB:1JBO}
RP X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, SUBUNIT, SUBCELLULAR LOCATION, AND METHYLATION AT ASN-72.
RX PubMed=12615541; DOI=10.1016/s1047-8477(02)00609-3;
RA Nield J., Rizkallah P.J., Barber J., Chayen N.E.;
RT "The 1.45 A three-dimensional structure of C-phycocyanin from the
RT thermophilic cyanobacterium Synechococcus elongatus.";
RL J. Struct. Biol. 141:149-155(2003).
RN [5] {ECO:0007744|PDB:3L0F}
RP X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RA Fromme R., Brune D., Fromme P.;
RT "The 3 dimensional structure of C-Phycocyanin at 1.35 A resolution.";
RL Submitted (DEC-2009) to the PDB data bank.
RN [6] {ECO:0007744|PDB:4Q70}
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RA Fromme R., Roy-Chowdhury S., Basu S., Yoon C., Brune D., Fromme P.;
RT "Observations on the Light Harvesting Protein Phycocyanin structure in high
RT resolution using a femtosecond X-Ray laser.";
RL Submitted (APR-2014) to the PDB data bank.
RN [7] {ECO:0007744|PDB:4Z8K}
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=26177184; DOI=10.1107/s2052252515009811;
RA Conrad C.E., Basu S., James D., Wang D., Schaffer A., Roy-Chowdhury S.,
RA Zatsepin N.A., Aquila A., Coe J., Gati C., Hunter M.S., Koglin J.E.,
RA Kupitz C., Nelson G., Subramanian G., White T.A., Zhao Y., Zook J.,
RA Boutet S., Cherezov V., Spence J.C., Fromme R., Weierstall U., Fromme P.;
RT "A novel inert crystal delivery medium for serial femtosecond
RT crystallography.";
RL IUCrJ 2:421-430(2015).
RN [8] {ECO:0007744|PDB:4ZIZ}
RP X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBCELLULAR LOCATION.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=26306196; DOI=10.1107/s2052252515013160;
RA Fromme R., Ishchenko A., Metz M., Chowdhury S.R., Basu S., Boutet S.,
RA Fromme P., White T.A., Barty A., Spence J.C., Weierstall U., Liu W.,
RA Cherezov V.;
RT "Serial femtosecond crystallography of soluble proteins in lipidic cubic
RT phase.";
RL IUCrJ 2:545-551(2015).
RN [9] {ECO:0007744|PDB:5UVK}
RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=28875031; DOI=10.1107/s205225251700570x;
RA Martin-Garcia J.M., Conrad C.E., Nelson G., Stander N., Zatsepin N.A.,
RA Zook J., Zhu L., Geiger J., Chun E., Kissick D., Hilgart M.C., Ogata C.,
RA Ishchenko A., Nagaratnam N., Roy-Chowdhury S., Coe J., Subramanian G.,
RA Schaffer A., James D., Ketwala G., Venugopalan N., Xu S., Corcoran S.,
RA Ferguson D., Weierstall U., Spence J.C.H., Cherezov V., Fromme P.,
RA Fischetti R.F., Liu W.;
RT "Serial millisecond crystallography of membrane and soluble protein
RT microcrystals using synchrotron radiation.";
RL IUCrJ 4:439-454(2017).
RN [10] {ECO:0007744|PDB:5MJM, ECO:0007744|PDB:5MJP, ECO:0007744|PDB:5MJQ, ECO:0007744|PDB:5O7M}
RP X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=NIES-2133 / IAM M-273 / BP-1;
RX PubMed=29097720; DOI=10.1038/s41467-017-01417-3;
RA Meents A., Wiedorn M.O., Srajer V., Henning R., Sarrou I., Bergtholdt J.,
RA Barthelmess M., Reinke P.Y.A., Dierksmeyer D., Tolstikova A., Schaible S.,
RA Messerschmidt M., Ogata C.M., Kissick D.J., Taft M.H., Manstein D.J.,
RA Lieske J., Oberthuer D., Fischetti R.F., Chapman H.N.;
RT "Pink-beam serial crystallography.";
RL Nat. Commun. 8:1281-1281(2017).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit, which further
CC assembles into trimers (PubMed:12460674, PubMed:12615541). The trimers
CC assemble into hexamers (Probable). {ECO:0000269|PubMed:12460674,
CC ECO:0000269|PubMed:12615541, ECO:0000305, ECO:0007744|PDB:1JBO,
CC ECO:0007744|PDB:1KTP, ECO:0007744|PDB:3L0F, ECO:0007744|PDB:4Q70,
CC ECO:0007744|PDB:4Z8K, ECO:0007744|PDB:4ZIZ, ECO:0007744|PDB:5MJM,
CC ECO:0007744|PDB:5MJP, ECO:0007744|PDB:5MJQ, ECO:0007744|PDB:5O7M,
CC ECO:0007744|PDB:5UVK}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:26306196}; Peripheral membrane protein
CC {ECO:0000269|PubMed:26306196}; Cytoplasmic side {ECO:0000250}.
CC Note=Part of the phycobilisome rod. {ECO:0000269|PubMed:12615541,
CC ECO:0000269|PubMed:26306196}.
CC -!- PTM: Contains two covalently linked bilin chromophores.
CC {ECO:0000269|PubMed:12460674, ECO:0000269|PubMed:12615541,
CC ECO:0000269|PubMed:26177184, ECO:0000269|PubMed:26306196,
CC ECO:0000269|PubMed:28875031, ECO:0007744|PDB:1JBO,
CC ECO:0007744|PDB:1KTP, ECO:0007744|PDB:3L0F, ECO:0007744|PDB:4Q70,
CC ECO:0007744|PDB:4Z8K, ECO:0007744|PDB:4ZIZ, ECO:0007744|PDB:5MJM,
CC ECO:0007744|PDB:5MJP, ECO:0007744|PDB:5MJQ, ECO:0007744|PDB:5O7M,
CC ECO:0007744|PDB:5UVK}.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; D13173; BAA02455.1; -; Genomic_DNA.
DR EMBL; BA000039; BAC09509.1; -; Genomic_DNA.
DR RefSeq; NP_682747.1; NC_004113.1.
DR RefSeq; WP_011057792.1; NC_004113.1.
DR PDB; 1I7Y; X-ray; 2.50 A; B=1-172.
DR PDB; 1JBO; X-ray; 1.45 A; B=1-172.
DR PDB; 1KTP; X-ray; 1.60 A; B=1-170.
DR PDB; 1ON7; X-ray; 2.70 A; B=1-172.
DR PDB; 3L0F; X-ray; 1.35 A; B=1-172.
DR PDB; 4Q70; X-ray; 1.85 A; B=1-172.
DR PDB; 4Z8K; X-ray; 2.50 A; B=1-172.
DR PDB; 4ZIZ; X-ray; 1.75 A; B=1-172.
DR PDB; 5MJM; X-ray; 2.30 A; B=1-172.
DR PDB; 5MJP; X-ray; 2.11 A; B=1-172.
DR PDB; 5MJQ; X-ray; 2.70 A; B=1-172.
DR PDB; 5O7M; X-ray; 2.46 A; B=1-172.
DR PDB; 5UVK; X-ray; 3.10 A; B=1-172.
DR PDB; 6YPQ; X-ray; 1.29 A; B=1-172.
DR PDB; 6YQ8; X-ray; 1.82 A; B=1-172.
DR PDB; 6YQG; X-ray; 1.45 A; B=1-172.
DR PDB; 6YYJ; X-ray; 2.16 A; B/D/F=1-172.
DR PDB; 7S50; X-ray; 2.10 A; B=1-172.
DR PDBsum; 1I7Y; -.
DR PDBsum; 1JBO; -.
DR PDBsum; 1KTP; -.
DR PDBsum; 1ON7; -.
DR PDBsum; 3L0F; -.
DR PDBsum; 4Q70; -.
DR PDBsum; 4Z8K; -.
DR PDBsum; 4ZIZ; -.
DR PDBsum; 5MJM; -.
DR PDBsum; 5MJP; -.
DR PDBsum; 5MJQ; -.
DR PDBsum; 5O7M; -.
DR PDBsum; 5UVK; -.
DR PDBsum; 6YPQ; -.
DR PDBsum; 6YQ8; -.
DR PDBsum; 6YQG; -.
DR PDBsum; 6YYJ; -.
DR PDBsum; 7S50; -.
DR AlphaFoldDB; P50033; -.
DR SMR; P50033; -.
DR MINT; P50033; -.
DR STRING; 197221.22295683; -.
DR EnsemblBacteria; BAC09509; BAC09509; BAC09509.
DR KEGG; tel:tlr1957; -.
DR PATRIC; fig|197221.4.peg.2047; -.
DR eggNOG; ENOG502Z7NE; Bacteria.
DR OMA; GITPGDC; -.
DR OrthoDB; 1307812at2; -.
DR EvolutionaryTrace; P50033; -.
DR Proteomes; UP000000440; Chromosome.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006247; Phycocyanin_b.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01339; phycocy_beta; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chromophore;
KW Electron transport; Membrane; Methylation; Photosynthesis; Phycobilisome;
KW Reference proteome; Thylakoid; Transport.
FT CHAIN 1..172
FT /note="C-phycocyanin beta subunit"
FT /id="PRO_0000199156"
FT BINDING 82
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="1"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0007744|PDB:1JBO, ECO:0007744|PDB:1KTP,
FT ECO:0007744|PDB:3L0F, ECO:0007744|PDB:4Q70,
FT ECO:0007744|PDB:4Z8K, ECO:0007744|PDB:4ZIZ,
FT ECO:0007744|PDB:5MJM, ECO:0007744|PDB:5MJP,
FT ECO:0007744|PDB:5MJQ, ECO:0007744|PDB:5O7M,
FT ECO:0007744|PDB:5UVK"
FT BINDING 153
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /ligand_label="2"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0007744|PDB:1JBO, ECO:0007744|PDB:1KTP,
FT ECO:0007744|PDB:4Q70, ECO:0007744|PDB:4Z8K,
FT ECO:0007744|PDB:4ZIZ, ECO:0007744|PDB:5MJM,
FT ECO:0007744|PDB:5MJP, ECO:0007744|PDB:5MJQ,
FT ECO:0007744|PDB:5O7M, ECO:0007744|PDB:5UVK"
FT MOD_RES 72
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000269|PubMed:12615541,
FT ECO:0007744|PDB:1JBO, ECO:0007744|PDB:1KTP"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 21..32
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 34..46
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 48..62
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 64..66
FT /evidence="ECO:0007829|PDB:6YPQ"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:5UVK"
FT HELIX 76..99
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 103..108
FT /evidence="ECO:0007829|PDB:6YPQ"
FT TURN 109..112
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 113..120
FT /evidence="ECO:0007829|PDB:6YPQ"
FT HELIX 124..142
FT /evidence="ECO:0007829|PDB:6YPQ"
FT STRAND 146..148
FT /evidence="ECO:0007829|PDB:4Q70"
FT HELIX 154..171
FT /evidence="ECO:0007829|PDB:6YPQ"
SQ SEQUENCE 172 AA; 18187 MW; 857B54F870800302 CRC64;
MLDAFAKVVA QADARGEFLT NAQFDALSNL VKEGNKRLDA VNRITSNAST IVANAARALF
AEQPQLIQPG GNAYTNRRMA ACLRDMEIIL RYVTYAILAG DSSVLDDRCL NGLRETYQAL
GTPGSSVAVA IQKMKDAAIA IANDPNGITP GDCSALMSEI AGYFDRAAAA VA
