ID   PHD_MYCS2               Reviewed;          65 AA.
AC   A0QRX9; I7F7Z9;
DT   09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Antitoxin Phd;
GN   Name=phd; OrderedLocusNames=MSMEG_1277, MSMEI_1239;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION AS AN ANTITOXIN, FUNCTION IN TRANSCRIPTION REGULATION, INDUCTION,
RP   AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22199354; DOI=10.1074/jbc.m111.286856;
RA   Frampton R., Aggio R.B., Villas-Boas S.G., Arcus V.L., Cook G.M.;
RT   "Toxin-antitoxin systems of Mycobacterium smegmatis are essential for cell
RT   survival.";
RL   J. Biol. Chem. 287:5340-5356(2012).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Neutralizes the bacteriostatic effect of cognate toxin Doc. In
CC       M.smegmatis 3 TA systems (VapB-VapC, MazE-MazF and Phd-Doc) may be
CC       involved in monitoring the nutritional supply and physiological state
CC       of the cell, linking catabolic with anabolic reactions.
CC       {ECO:0000269|PubMed:22199354}.
CC   -!- INDUCTION: Constitutively expressed in all growth phases, part of the
CC       MSMEG_1276-phd-doc-MSMEG_1279 operon. Negatively autoregulated by one
CC       or both of Phd-Doc. {ECO:0000269|PubMed:22199354}.
CC   -!- DISRUPTION PHENOTYPE: The phd-doc operon is not essential. A triple TA
CC       mutant (missing vapB-vapC, mazE-mazF and phd-doc TA systems) survives
CC       antibiotic challenge, suggesting the TA systems are not required to
CC       generate drug-resistant cells. However the mutant is more sensitive to
CC       oxidative and heat stress, and does not survive long term starvation
CC       during aerobic growth on complex medium. There is a difference in the
CC       level of branched-chain amino acids, which may play a role in
CC       monitoring the nutritional supply and physiological state of the cell.
CC       {ECO:0000269|PubMed:22199354}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFP37715.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK73827.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP37715.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011727559.1; NZ_SIJM01000042.1.
DR   RefSeq; YP_885667.1; NC_008596.1.
DR   AlphaFoldDB; A0QRX9; -.
DR   SMR; A0QRX9; -.
DR   STRING; 246196.MSMEI_1239; -.
DR   EnsemblBacteria; ABK73827; ABK73827; MSMEG_1277.
DR   EnsemblBacteria; AFP37715; AFP37715; MSMEI_1239.
DR   GeneID; 66732739; -.
DR   KEGG; msg:MSMEI_1239; -.
DR   KEGG; msm:MSMEG_1277; -.
DR   PATRIC; fig|246196.19.peg.1266; -.
DR   eggNOG; ENOG50347Y8; Bacteria.
DR   OMA; PAMNVEF; -.
DR   OrthoDB; 2085054at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
PE   1: Evidence at protein level;
KW   Reference proteome; Repressor; Toxin-antitoxin system; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..65
FT                   /note="Antitoxin Phd"
FT                   /id="PRO_0000420848"
SQ   SEQUENCE   65 AA;  7276 MW;  CCBCC2C5737EA1CD CRC64;
     MPSLNIDFDE AEMEQIRAAA RADDLSLKKF AHAAVMERAS AHKRRVAEAA RLVAERSAEL
     NRRLA