PHE3_RHDS2
ID PHE3_RHDS2 Reviewed; 128 AA.
AC Q00433; O82050;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Phycoerythrin alpha-3 chain, chloroplastic;
DE Flags: Precursor;
GN Name=cpeA3;
OS Rhodomonas sp. (strain CS 24) (Chroomonas sp. (strain CS24)).
OC Eukaryota; Cryptophyceae; Pyrenomonadales; Pyrenomonadaceae; Rhodomonas;
OC unclassified Rhodomonas.
OX NCBI_TaxID=79257;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2226853; DOI=10.1016/0014-5793(90)81082-y;
RA Jenkins J., Hiller R.G., Speirs J., Godovac-Zimmermann J.;
RT "A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit.
RT Evidence for three alpha-subunits and an N-terminal membrane transit
RT sequence.";
RL FEBS Lett. 273:191-194(1990).
RN [2]
RP ERRATUM OF PUBMED:2226853.
RX PubMed=2004659; DOI=10.1016/0014-5793(91)80187-8;
RA Jenkins J., Hiller R.G., Speirs J., Godovac-Zimmermann J.;
RL FEBS Lett. 279:361-361(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION TO 62.
RA Hiller R.G., Howe C.E.;
RT "Nuclear-encoded genes of phycoerythrin alpha subunits.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) OF 53-128 IN COMPLEX WITH CPEA2;
RP CPEB AND 15,16-DIHYDROBILIVERDIN, SUBUNIT, AND HYDROXYLATION AT LYS-56.
RX PubMed=10430868; DOI=10.1073/pnas.96.16.8901;
RA Wilk K.E., Harrop S.J., Jankova L., Edler D., Keenan G., Sharples F.,
RA Hiller R.G., Curmi P.M.;
RT "Evolution of a light-harvesting protein by addition of new subunits and
RT rearrangement of conserved elements: crystal structure of a cryptophyte
RT phycoerythrin at 1.63-A resolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:8901-8906(1999).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS) OF 53-128 IN COMPLEX WITH CPEA2;
RP CPEB AND 15,16-DIHYDROBILIVERDIN, SUBUNIT, AND HYDROXYLATION AT LYS-56.
RX PubMed=15504407; DOI=10.1016/j.jmb.2004.09.044;
RA Doust A.B., Marai C.N., Harrop S.J., Wilk K.E., Curmi P.M., Scholes G.D.;
RT "Developing a structure-function model for the cryptophyte phycoerythrin
RT 545 using ultrahigh resolution crystallography and ultrafast laser
RT spectroscopy.";
RL J. Mol. Biol. 344:135-153(2004).
CC -!- FUNCTION: Light-harvesting photosynthetic tetrapyrrole chromophore-
CC protein from the phycobiliprotein complex.
CC -!- SUBUNIT: Heterotetramer of 2 different alpha chains and 2 identical
CC beta chains. The subunit composition could comprise of any combination
CC of 2 out of 4 different alpha units with an invariant beta unit.
CC {ECO:0000269|PubMed:10430868, ECO:0000269|PubMed:15504407}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane;
CC Peripheral membrane protein; Lumenal side.
CC -!- PTM: Contains one covalently linked 15,16-dihydrobiliverdin
CC chromophore.
CC -!- MISCELLANEOUS: The light-harvesting system in Cryptophytes contains
CC phycobiliprotein complexes. Unusually they are composed of either
CC phycoerythrin (CPE) or phycocyanin (CPC) but never allophycocyanin
CC (APC), with only one type of biliprotein being present in any one
CC species. Unlike cyanobacteria or red algae these proteins are not
CC arranged into higher-order phycobilisome complexes, and they are found
CC in the thylakoid lumen.
CC -!- SIMILARITY: Belongs to the phycoerythrin family. {ECO:0000305}.
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DR EMBL; AJ006994; CAA07415.2; -; Genomic_DNA.
DR PIR; S12900; S12900.
DR PDB; 1QGW; X-ray; 1.63 A; A=53-128.
DR PDB; 1XF6; X-ray; 1.10 A; A=53-128.
DR PDB; 1XG0; X-ray; 0.97 A; A=53-128.
DR PDBsum; 1QGW; -.
DR PDBsum; 1XF6; -.
DR PDBsum; 1XG0; -.
DR AlphaFoldDB; Q00433; -.
DR SMR; Q00433; -.
DR EvolutionaryTrace; Q00433; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030089; C:phycobilisome; IEA:InterPro.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 3.90.510.10; -; 1.
DR InterPro; IPR011070; Globular_prot_asu/bsu.
DR InterPro; IPR037011; Phycoerythr-like_a_sf.
DR InterPro; IPR004228; Phycoerythr_a.
DR InterPro; IPR006311; TAT_signal.
DR Pfam; PF02972; Phycoerythr_ab; 1.
DR SUPFAM; SSF56568; SSF56568; 1.
DR PROSITE; PS51318; TAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bile pigment; Chloroplast; Chromophore; Electron transport;
KW Hydroxylation; Membrane; Photosynthesis; Plastid; Thylakoid;
KW Transit peptide; Transport.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT CHAIN 53..128
FT /note="Phycoerythrin alpha-3 chain, chloroplastic"
FT /id="PRO_0000002828"
FT REGION 70..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 71
FT /ligand="15,16-dihydrobiliverdin"
FT /ligand_id="ChEBI:CHEBI:57899"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:10430868,
FT ECO:0000269|PubMed:15504407"
FT BINDING 73
FT /ligand="15,16-dihydrobiliverdin"
FT /ligand_id="ChEBI:CHEBI:57899"
FT /evidence="ECO:0000269|PubMed:10430868,
FT ECO:0000269|PubMed:15504407"
FT BINDING 77..78
FT /ligand="15,16-dihydrobiliverdin"
FT /ligand_id="ChEBI:CHEBI:57899"
FT BINDING 93
FT /ligand="15,16-dihydrobiliverdin"
FT /ligand_id="ChEBI:CHEBI:57899"
FT /evidence="ECO:0000269|PubMed:10430868,
FT ECO:0000269|PubMed:15504407"
FT MOD_RES 56
FT /note="5-hydroxylysine"
FT /evidence="ECO:0000269|PubMed:10430868,
FT ECO:0000269|PubMed:15504407"
FT STRAND 59..67
FT /evidence="ECO:0007829|PDB:1XG0"
FT HELIX 86..89
FT /evidence="ECO:0007829|PDB:1XG0"
FT STRAND 90..98
FT /evidence="ECO:0007829|PDB:1XG0"
FT HELIX 103..113
FT /evidence="ECO:0007829|PDB:1XG0"
FT STRAND 120..122
FT /evidence="ECO:0007829|PDB:1XG0"
SQ SEQUENCE 128 AA; 13175 MW; 44B1ECFD62C12732 CRC64;
MFAKTLASLA VIGSAAAYVP MMSMDMGRRE VVQAGAAAAA VTPFLSGAPA GAAMDKSAKA
PQITIFDHRG CSRAPKESTG GKAGGQDDEM MVKVASTKVT VSESDAAKKL QEFITFEKGI
DGPFTSKN
