PHEA_LACLM
ID PHEA_LACLM Reviewed; 279 AA.
AC P43909; A2RMG3;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Prephenate dehydratase;
DE Short=PDT;
DE EC=4.2.1.51;
GN Name=pheA; OrderedLocusNames=llmg_1924;
OS Lactococcus lactis subsp. cremoris (strain MG1363).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus; Lactococcus cremoris subsp. cremoris.
OX NCBI_TaxID=416870;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MG1363 / F15876;
RX PubMed=7823907; DOI=10.1007/bf00290140;
RA Griffin H.G., Gasson M.J.;
RT "Genetic aspects of aromatic amino acid biosynthesis in Lactococcus
RT lactis.";
RL Mol. Gen. Genet. 246:119-127(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MG1363;
RX PubMed=17307855; DOI=10.1128/jb.01768-06;
RA Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA van Sinderen D., Kok J.;
RT "The complete genome sequence of the lactic acid bacterial paradigm
RT Lactococcus lactis subsp. cremoris MG1363.";
RL J. Bacteriol. 189:3256-3270(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O;
CC Xref=Rhea:RHEA:21648, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:18005, ChEBI:CHEBI:29934; EC=4.2.1.51;
CC -!- PATHWAY: Amino-acid biosynthesis; L-phenylalanine biosynthesis;
CC phenylpyruvate from prephenate: step 1/1.
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DR EMBL; X78413; CAA55182.1; -; Genomic_DNA.
DR EMBL; AM406671; CAL98493.1; -; Genomic_DNA.
DR PIR; S52582; S52582.
DR RefSeq; WP_011835672.1; NZ_WJVF01000007.1.
DR AlphaFoldDB; P43909; -.
DR SMR; P43909; -.
DR STRING; 416870.llmg_1924; -.
DR PRIDE; P43909; -.
DR EnsemblBacteria; CAL98493; CAL98493; llmg_1924.
DR KEGG; llm:llmg_1924; -.
DR eggNOG; COG0077; Bacteria.
DR HOGENOM; CLU_035008_0_2_9; -.
DR OMA; PLMIYRE; -.
DR PhylomeDB; P43909; -.
DR BioCyc; LLAC416870:LLMG_RS09630-MON; -.
DR UniPathway; UPA00121; UER00345.
DR Proteomes; UP000000364; Chromosome.
DR GO; GO:0004664; F:prephenate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009094; P:L-phenylalanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR001086; Preph_deHydtase.
DR InterPro; IPR018528; Preph_deHydtase_CS.
DR Pfam; PF00800; PDT; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR PROSITE; PS51671; ACT; 1.
DR PROSITE; PS00857; PREPHENATE_DEHYDR_1; 1.
DR PROSITE; PS00858; PREPHENATE_DEHYDR_2; 1.
DR PROSITE; PS51171; PREPHENATE_DEHYDR_3; 1.
PE 4: Predicted;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Lyase;
KW Phenylalanine biosynthesis.
FT CHAIN 1..279
FT /note="Prephenate dehydratase"
FT /id="PRO_0000119179"
FT DOMAIN 2..178
FT /note="Prephenate dehydratase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00517"
FT DOMAIN 194..272
FT /note="ACT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT SITE 171
FT /note="Essential for activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 279 AA; 31030 MW; A11ED51557A0AD73 CRC64;
MKIAYLGPRG SFCSVVAEAA FKSEELYSYA TILDVIEAYN EGECDFALVP IENSTEGTVN
MSIDKIFHDS NAKVVAEFVL PISQNLLAVS KEQKIEHIYS HPQALAQTRV YLRKFYPQAQ
VEITESTSAA AEFVKNNPDL PAAAVANSFA AKMYDLEFIA ENIQDLAGNS TRFWLLGKEK
QSFDLNQTKD KVTLALTLPD NLPGALHKAI SVFAWRDIDM TKIESRPLRT RLGQYFFIID
LENNATNSLK IPYALEELAG LGVNVRLLGN YSVYSLGEV
