PHEA_MYCA9
ID PHEA_MYCA9 Reviewed; 308 AA.
AC B1MEG8;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Prephenate dehydratase;
DE Short=PDT;
DE EC=4.2.1.51;
GN Name=pheA; OrderedLocusNames=MAB_0132;
OS Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM
OS 13569 / NCTC 13031 / TMC 1543) (Mycobacterium abscessus).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacteroides; Mycobacteroides abscessus.
OX NCBI_TaxID=561007;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC
RC 1543;
RX PubMed=19543527; DOI=10.1371/journal.pone.0005660;
RA Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M.,
RA Macheras E., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L.,
RA Gaillard J.L.;
RT "Non mycobacterial virulence genes in the genome of the emerging pathogen
RT Mycobacterium abscessus.";
RL PLoS ONE 4:E5660-E5660(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O;
CC Xref=Rhea:RHEA:21648, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:18005, ChEBI:CHEBI:29934; EC=4.2.1.51;
CC -!- PATHWAY: Amino-acid biosynthesis; L-phenylalanine biosynthesis;
CC phenylpyruvate from prephenate: step 1/1.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
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DR EMBL; CU458896; CAM60232.1; -; Genomic_DNA.
DR RefSeq; WP_005062863.1; NZ_MLCG01000005.1.
DR AlphaFoldDB; B1MEG8; -.
DR SMR; B1MEG8; -.
DR EnsemblBacteria; CAM60232; CAM60232; MAB_0132.
DR GeneID; 66970516; -.
DR KEGG; mab:MAB_0132; -.
DR OMA; PLMIYRE; -.
DR UniPathway; UPA00121; UER00345.
DR Proteomes; UP000007137; Chromosome.
DR GO; GO:0004106; F:chorismate mutase activity; IEA:InterPro.
DR GO; GO:0004664; F:prephenate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0009094; P:L-phenylalanine biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR008242; Chor_mutase/pphenate_deHydtase.
DR InterPro; IPR001086; Preph_deHydtase.
DR InterPro; IPR018528; Preph_deHydtase_CS.
DR Pfam; PF01842; ACT; 1.
DR Pfam; PF00800; PDT; 1.
DR PIRSF; PIRSF001500; Chor_mut_pdt_Ppr; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR PROSITE; PS51671; ACT; 1.
DR PROSITE; PS00858; PREPHENATE_DEHYDR_2; 1.
DR PROSITE; PS51171; PREPHENATE_DEHYDR_3; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Lyase;
KW Phenylalanine biosynthesis.
FT CHAIN 1..308
FT /note="Prephenate dehydratase"
FT /id="PRO_0000382030"
FT DOMAIN 3..187
FT /note="Prephenate dehydratase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00517"
FT DOMAIN 201..278
FT /note="ACT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT SITE 180
FT /note="Essential for activity"
FT /evidence="ECO:0000250"
SQ SEQUENCE 308 AA; 32178 MW; 7D02AB44EB0D724F CRC64;
MQRITYLGPE GTFSEAAMIT LRTTGRIPGS SEVEPVSVAS AREALVQVQA GDADYACVPI
ESSLEGPVVP TLDTLAVGAP LQIFAETVLP VSFTIAVRPG TAAGDVKTVA GFPIAAAQVR
EWLATNLPDA ELVAANSNAA AAEDVKAERA DAGVCTEWAA QRLGLHALAS GVVDEAHAHT
RFVLVGRPGP PPAATGADRT SVVLGLGNVP GALAAAMNEF AIRDIDLTRI ESRPTRTGLG
TYRFFLDCVG HIDDIAVGEA LKGLHRRCED VRYLGSWPRG TTAPTGANPP VLDEASGWLA
ETREGRLR
