ID   PHEA_PSEUE              Reviewed;         607 AA.
AC   P31020;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Phenol 2-monooxygenase;
DE            EC=1.14.13.7;
DE   AltName: Full=Phenol hydroxylase;
GN   Name=pheA;
OS   Pseudomonas sp. (strain EST1001).
OG   Plasmid pEST1412.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=69012;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1650730; DOI=10.1016/0378-1119(91)90531-f;
RA   Nurk A., Kasak L., Kivisaar M.;
RT   "Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas
RT   sp. EST1001: expression in Escherichia coli and Pseudomonas putida.";
RL   Gene 102:13-18(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + NADPH + O2 + phenol = catechol + H2O + NADP(+);
CC         Xref=Rhea:RHEA:17061, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:15882, ChEBI:CHEBI:18135,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.14.13.7;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- PATHWAY: Aromatic compound metabolism; phenol degradation.
CC   -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; M57500; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; JQ1132; JQ1132.
DR   AlphaFoldDB; P31020; -.
DR   SMR; P31020; -.
DR   PRIDE; P31020; -.
DR   UniPathway; UPA00728; -.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0018662; F:phenol 2-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019336; P:phenol-containing compound catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; FAD; Flavoprotein; Monooxygenase; NADP;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..607
FT                   /note="Phenol 2-monooxygenase"
FT                   /id="PRO_0000214046"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         37..66
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   BINDING         319..329
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   607 AA;  67419 MW;  E030D692C96EBAF6 CRC64;
     MTTQRNDNLE QPGRSVIFDD GLSATDTPNE TNVVETEVLI VGSGPAGSSA AMFLSTQGIS
     NIMITKYRWT ANTPRAHITN QRTMEILRDA GIEDQVLAEA VPHELMGDTV YCESMAGEEI
     GRRPTWGTRP DRRADYELAS PAMPCDIPQT LLEPIMLKNA TMRGTQTQFS TEYLSHTQDD
     KGVSVQVLNR LTGQEYTIRA KYLIGADGAR SKVAADIGGS MNITFKADLS HWRPSALDPV
     LGLPPRIEYR WPRRWFDRMV RPWNEWLVVW GFDINQEPPK LNDDEAIQIV RNLVGIEDLD
     VEILGYSLWG NNDQYATHLQ KGRVCCAGDA IHKHPPSHGL GSNTSIQDSY NLCWKLACVL
     KGQAGPELLE TYSTERAPIA KQIVTRANGS SSEYKPIFDA LGVTDATTND EFVEKLALRK
     ENSPEGARRR AALRAALDNK DYEFNAQGTE IGQFYDSSAV ITDGQKRPAM TEDPMLHHQK
     STFPGLRLPH AWLGDAKEKY STHDIAEGTR FTIFTGITGQ AWADAAVRVA ERLGIDLKAV
     VIGEGQPVQD LYGDWLRQRE VDEDGVILVR PDKHIGWRAQ SMVADPETAL FDVLSGCCIP
     SKPALRI