PHEB_PORPP
ID PHEB_PORPP Reviewed; 177 AA.
AC P11393;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=B-phycoerythrin beta chain;
GN Name=cpeB;
OS Porphyridium purpureum (Red alga) (Porphyridium cruentum).
OG Plastid; Chloroplast.
OC Eukaryota; Rhodophyta; Bangiophyceae; Porphyridiales; Porphyridiaceae;
OC Porphyridium.
OX NCBI_TaxID=35688;
RN [1]
RP PROTEIN SEQUENCE, AND METHYLATION AT ASN-72.
RX PubMed=2495805; DOI=10.1515/bchm3.1989.370.1.115;
RA Sidler W., Kumpf B., Suter F., Klotz A.V., Glazer A.N., Zuber H.;
RT "The complete amino-acid sequence of the alpha and beta subunits of B-
RT phycoerythrin from the rhodophytan alga Porphyridium cruentum.";
RL Biol. Chem. Hoppe-Seyler 370:115-124(1989).
RN [2]
RP PROTEIN SEQUENCE OF 38-77; 79-84 AND 151-171, AND CHROMOPHORE ATTACHMENT
RP SITES.
RX PubMed=6715353; DOI=10.1016/s0021-9258(18)91035-5;
RA Lundell D.J., Glazer A.N., DeLange R.J., Brown D.M.;
RT "Bilin attachment sites in the alpha and beta subunits of B-phycoerythrin.
RT Amino acid sequence studies.";
RL J. Biol. Chem. 259:5472-5480(1984).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex.
CC -!- SUBUNIT: Heteromer of 6 alpha, 6 beta and one gamma chain.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Stromal side
CC {ECO:0000250}. Note=Forms the periphery of the phycobilisome rod.
CC {ECO:0000250}.
CC -!- PTM: Contains two covalently linked phycoerythrobilin chromophores and
CC one covalently linked phycourobilin chromophore.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR PIR; S02818; S02818.
DR RefSeq; YP_008965770.1; NC_023133.1.
DR PDB; 3V57; X-ray; 1.70 A; B/D=1-177.
DR PDB; 3V58; X-ray; 1.85 A; B/D=1-177.
DR PDB; 6KGX; EM; 2.80 A; 11/14/A5/AC/B1/B3/B4/B5/BC/BD/BE/BG/C5/C9/CC/CJ/D1/D3/D4/D8/DA/DD/DE/DG/E9/EJ/F1/F3/F4/F5=1-177.
DR PDB; 7LIX; EM; 2.80 A; B/C/D=1-177.
DR PDB; 7LIY; EM; 2.80 A; B/C=1-177.
DR PDB; 7LIZ; EM; 2.80 A; B/C=1-177.
DR PDB; 7LJ0; EM; 2.80 A; B=1-177.
DR PDBsum; 3V57; -.
DR PDBsum; 3V58; -.
DR PDBsum; 6KGX; -.
DR PDBsum; 7LIX; -.
DR PDBsum; 7LIY; -.
DR PDBsum; 7LIZ; -.
DR PDBsum; 7LJ0; -.
DR AlphaFoldDB; P11393; -.
DR SMR; P11393; -.
DR IntAct; P11393; 1.
DR MINT; P11393; -.
DR iPTMnet; P11393; -.
DR GeneID; 17963946; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chloroplast; Chromophore;
KW Direct protein sequencing; Electron transport; Membrane; Methylation;
KW Photosynthesis; Phycobilisome; Plastid; Thylakoid; Transport.
FT CHAIN 1..177
FT /note="B-phycoerythrin beta chain"
FT /id="PRO_0000199194"
FT BINDING 50
FT /ligand="phycourobilin"
FT /ligand_id="ChEBI:CHEBI:189062"
FT /note="covalent, via 2 links"
FT BINDING 61
FT /ligand="phycourobilin"
FT /ligand_id="ChEBI:CHEBI:189062"
FT /note="covalent, via 2 links"
FT BINDING 82
FT /ligand="(2R,3E)-phycoerythrobilin"
FT /ligand_id="ChEBI:CHEBI:85276"
FT /ligand_label="1"
FT /note="covalent, via 1 link"
FT BINDING 158
FT /ligand="(2R,3E)-phycoerythrobilin"
FT /ligand_id="ChEBI:CHEBI:85276"
FT /ligand_label="2"
FT /note="covalent, via 1 link"
FT MOD_RES 72
FT /note="N4-methylasparagine"
FT /evidence="ECO:0000269|PubMed:2495805"
FT CONFLICT 167
FT /note="S -> Q (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:3V57"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:7LIX"
FT HELIX 21..32
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 34..45
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 48..62
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 76..99
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 103..108
FT /evidence="ECO:0007829|PDB:3V57"
FT TURN 109..112
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 113..120
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 124..142
FT /evidence="ECO:0007829|PDB:3V57"
FT HELIX 159..176
FT /evidence="ECO:0007829|PDB:3V57"
SQ SEQUENCE 177 AA; 18554 MW; 168E71372A29CD89 CRC64;
MLDAFSRVVV NSDAKAAYVG GSDLQALKSF IADGNKRLDA VNSIVSNASC MVSDAVSGMI
CENPGLISPG GNCYTNRRMA ACLRDGEIIL RYVSYALLAG DASVLEDRCL NGLKETYIAL
GVPTNSSIRA VSIMKAQAVA FITNTATERK MSFAAGDCTS LASEVASYFD RVGAAIS
