ID   PHEB_PSEUE              Reviewed;         302 AA.
AC   P31019;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Catechol 1,2-dioxygenase;
DE            EC=1.13.11.1;
DE   AltName: Full=1,2-CTD;
GN   Name=pheB;
OS   Pseudomonas sp. (strain EST1001).
OG   Plasmid pEST1412.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=69012;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2013408; DOI=10.1016/0378-1119(91)90098-v;
RA   Kivisaar M., Kasak L., Nurk A.;
RT   "Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene,
RT   pheB, of phenol-degrading Pseudomonas sp. strain EST1001.";
RL   Gene 98:15-20(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=catechol + O2 = cis,cis-muconate + 2 H(+);
CC         Xref=Rhea:RHEA:23852, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:18135, ChEBI:CHEBI:32379; EC=1.13.11.1;
CC   -!- COFACTOR:
CC       Name=Fe(3+); Xref=ChEBI:CHEBI:29034;
CC       Note=Binds 1 Fe(3+) ion per subunit.;
CC   -!- PATHWAY: Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-
CC       4,5-dihydro-2-furylacetate from catechol: step 1/3.
CC   -!- SIMILARITY: Belongs to the intradiol ring-cleavage dioxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; M57500; AAC64900.1; -; Genomic_DNA.
DR   PIR; JN0143; JN0143.
DR   AlphaFoldDB; P31019; -.
DR   SMR; P31019; -.
DR   UniPathway; UPA00157; UER00258.
DR   GO; GO:0018576; F:catechol 1,2-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008199; F:ferric iron binding; IEA:InterPro.
DR   GO; GO:0042952; P:beta-ketoadipate pathway; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019614; P:catechol-containing compound catabolic process; IEA:InterPro.
DR   CDD; cd03460; 1_2-CTD; 1.
DR   Gene3D; 2.60.130.10; -; 1.
DR   InterPro; IPR007535; Catechol_dOase_N.
DR   InterPro; IPR012801; Cchol_dOase_prob.
DR   InterPro; IPR000627; Intradiol_dOase_C.
DR   InterPro; IPR015889; Intradiol_dOase_core.
DR   Pfam; PF00775; Dioxygenase_C; 1.
DR   Pfam; PF04444; Dioxygenase_N; 1.
DR   SUPFAM; SSF49482; SSF49482; 1.
DR   TIGRFAMs; TIGR02439; catechol_proteo; 1.
DR   PROSITE; PS00083; INTRADIOL_DIOXYGENAS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Metal-binding;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..302
FT                   /note="Catechol 1,2-dioxygenase"
FT                   /id="PRO_0000085085"
FT   BINDING         164
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         198
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         222
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         224
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   302 AA;  33362 MW;  A86F17E68D1EAC3A CRC64;
     MTVKIYDTPE VQDFLKIVAG LDQEGGNDRG KQIIHRILSD LYRTIDDFDI TAEQYWSAVS
     LLNALGQASQ FGLLSPGLGF DHYMDMRMDA ADAEAKRTGG TPRTIEGPLY VAGAPEAEGF
     ARMDDDPDTD GETMWLHGQV RDTAGKPIPG AKVEIWHCNS KGGYSFFDKS QTPYNLRRTI
     IADNEGYYRA RSVIPSGYGV PEGAPTDQVL KLLGRHGERP AHIHYFISAP GHQHLTTQIN
     LAGDPYTYDD FAFATRQDLA AEGKRVENHP AAQQYGVEGT VTEVIFNIEL SPTAEEELQA
     RP