ID   PHE_STRMO               Reviewed;          14 AA.
AC   P81801;
DT   13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Puromycin-hydrolyzing enzyme;
DE            EC=3.-.-.-;
DE   Flags: Fragment;
OS   Streptomyces morookaense (Streptoverticillium morookaense).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1970;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 19166 / DSM 40503 / JCM 4673 / KCC S-0673 / NBRC 13416 / NRRL
RC   B-12429 / VKM Ac-1916;
RX   PubMed=9538199; DOI=10.1093/oxfordjournals.jbchem.a021929;
RA   Nishimura M., Matsuo H., Nakamura A., Sugiyama M.;
RT   "Purification and characterization of a puromycin-hydrolyzing enzyme from
RT   blasticidin S-producing Streptomyces morookaensis.";
RL   J. Biochem. 123:247-252(1998).
RN   [2]
RP   CHARACTERIZATION, AND FUNCTION.
RA   Nishimura M., Matsuo H., Sugiyama M.;
RT   "Blasticidin S-producing Streptomyces morookaensis possesses an enzyme
RT   activity with hydrolyzes puromycin.";
RL   FEMS Microbiol. Lett. 132:95-100(1995).
CC   -!- FUNCTION: Inactivates puromycin by catalyzing the hydrolysis of the
CC       amide linkage between its aminonucleoside and O-methyl-L-tyrosine
CC       moieties. May have aminopeptidase activity. {ECO:0000269|Ref.2}.
CC   -!- ACTIVITY REGULATION: Stimulated by DTT. Strongly inhibited by zinc ion,
CC       Fe(2+) ion, Cu(2+) ion, mercury ion, N-bromosuccinimide and N-
CC       ethylmaleimide. Partially inhibited by cobalt ion.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.0.;
CC       Temperature dependence:
CC         Optimum temperature is 45 degrees Celsius.;
CC   -!- SUBUNIT: Monomer.
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DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Aminopeptidase; Direct protein sequencing; Hydrolase; Protease.
FT   CHAIN           1..>14
FT                   /note="Puromycin-hydrolyzing enzyme"
FT                   /id="PRO_0000058378"
FT   NON_TER         14
SQ   SEQUENCE   14 AA;  1492 MW;  3F980730E45EF3D8 CRC64;
     VSTAPYGAWQ SPID