ID   PHIA_EMENI              Reviewed;         182 AA.
AC   Q5ATP7; C8VE25; Q8J1V2;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Cell wall protein phiA {ECO:0000305};
DE   AltName: Full=Bafilomycin-induced protein B {ECO:0000303|PubMed:10376827};
DE   AltName: Full=Phialide development protein A {ECO:0000303|PubMed:14599891};
DE   Flags: Precursor;
GN   Name=phiA {ECO:0000303|PubMed:14599891};
GN   Synonyms=binB {ECO:0000303|PubMed:10376827}; ORFNames=ANIA_08333;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, TISSUE
RP   SPECIFICITY, FUNCTION, INDUCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=14599891; DOI=10.1016/s1087-1845(03)00108-7;
RA   Melin P., Schnuerer J., Wagner E.G.;
RT   "Characterization of phiA, a gene essential for phialide development in
RT   Aspergillus nidulans.";
RL   Fungal Genet. Biol. 40:234-241(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [4]
RP   INDUCTION.
RX   PubMed=10376827; DOI=10.1099/13500872-145-5-1115;
RA   Melin P., Schnuerer J., Wagner E.G.;
RT   "Changes in Aspergillus nidulans gene expression induced by bafilomycin, a
RT   Streptomyces-produced antibiotic.";
RL   Microbiology 145:1115-1122(1999).
RN   [5]
RP   INDUCTION.
RX   PubMed=12207217; DOI=10.1007/s00438-002-0695-0;
RA   Melin P., Schnuerer J., Wagner E.G.H.;
RT   "Proteome analysis of Aspergillus nidulans reveals proteins associated with
RT   the response to the antibiotic concanamycin A, produced by Streptomyces
RT   species.";
RL   Mol. Genet. Genomics 267:695-702(2002).
CC   -!- FUNCTION: Cell wall protein involved in development of asexual
CC       structures such as phialide and conidium development, and thus required
CC       for spore formation (PubMed:14599891). Plays a role as a general stress
CC       protectant produced by the fungus in competition with antagonistic
CC       bacteria (By similarity). {ECO:0000250|UniProtKB:A2R2S8,
CC       ECO:0000269|PubMed:14599891}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:D4AV66}.
CC       Secreted, cell wall {ECO:0000269|PubMed:14599891}.
CC   -!- TISSUE SPECIFICITY: Mainly present in phialides and conidia.
CC       {ECO:0000269|PubMed:14599891}.
CC   -!- INDUCTION: Expression is highly induced by the V-ATPase inhibitor
CC       bafilomycin B1 and by oncanamycin A (PubMed:10376827, PubMed:12207217).
CC       The promoter contains two abaA ATTS DNA binding motifs
CC       (PubMed:14599891). {ECO:0000269|PubMed:10376827,
CC       ECO:0000269|PubMed:12207217, ECO:0000269|PubMed:14599891}.
CC   -!- DISRUPTION PHENOTYPE: Leads to reduced sporulation and division of
CC       phialides instead of forming a single flask-shaped cell.
CC       {ECO:0000269|PubMed:14599891}.
CC   -!- SIMILARITY: Belongs to the phiA family. {ECO:0000305}.
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DR   EMBL; AJ511280; CAD54043.1; -; Genomic_DNA.
DR   EMBL; BN001305; CBF80327.1; -; Genomic_DNA.
DR   EMBL; AACD01000150; EAA66956.1; -; Genomic_DNA.
DR   RefSeq; XP_681602.1; XM_676510.1.
DR   AlphaFoldDB; Q5ATP7; -.
DR   STRING; 162425.CADANIAP00002798; -.
DR   EnsemblFungi; CBF80327; CBF80327; ANIA_08333.
DR   EnsemblFungi; EAA66956; EAA66956; AN8333.2.
DR   GeneID; 2868754; -.
DR   KEGG; ani:AN8333.2; -.
DR   VEuPathDB; FungiDB:AN8333; -.
DR   eggNOG; ENOG502SNZ2; Eukaryota.
DR   HOGENOM; CLU_097238_0_0_1; -.
DR   InParanoid; Q5ATP7; -.
DR   OMA; WSIWADA; -.
DR   OrthoDB; 1526873at2759; -.
DR   Proteomes; UP000000560; Chromosome V.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IDA:AspGD.
DR   GO; GO:0070787; P:conidiophore development; IMP:AspGD.
DR   GO; GO:0070790; P:phialide development; IMP:AspGD.
DR   GO; GO:0043935; P:sexual sporulation resulting in formation of a cellular spore; IMP:AspGD.
DR   GO; GO:0000905; P:sporocarp development involved in asexual reproduction; IMP:AspGD.
PE   2: Evidence at transcript level;
KW   Cell wall; Glycoprotein; Reference proteome; Secreted; Signal; Sporulation.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..182
FT                   /note="Cell wall protein phiA"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000434419"
FT   CARBOHYD        57
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        107
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CONFLICT        33
FT                   /note="S -> T (in Ref. 1; CAD54043)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   182 AA;  18770 MW;  9B7D9FE05B6ED0BC CRC64;
     MKLTSTAALA SLAVAATAAP STPETFGLVA IRSGDAVQYA GFNAALGSIF AGLPKQNATC
     EGTDSGFATF YIKDGALYLY GSEETQEIYV DRSGMGQGLI GYTTGDNASG PRNSERTGWS
     IDENNHLVFD GNSLIACPNS IDSAYSIWAS AGVANPGGNK DCVGIAARVE KTENPIACTY
     SS