ID   PHI_MYCGS               Reviewed;         199 AA.
AC   Q9LBW5;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=3-hexulose-6-phosphate isomerase;
DE            EC=5.3.1.27;
DE   AltName: Full=6-phospho-3-hexuloisomerase;
DE            Short=PHI;
GN   Name=rmpB;
OS   Mycobacterium gastri.
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=1777;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TRANSCRIPTIONAL
RP   REGULATION.
RC   STRAIN=MB19;
RX   PubMed=10648518; DOI=10.1128/jb.182.4.944-948.2000;
RA   Mitsui R., Sakai Y., Yasueda H., Kato N.;
RT   "A novel operon encoding formaldehyde fixation: the ribulose monophosphate
RT   pathway in the Gram-positive facultative methylotrophic bacterium
RT   Mycobacterium gastri MB19.";
RL   J. Bacteriol. 182:944-948(2000).
CC   -!- FUNCTION: Catalyzes the isomerization between 3-hexulose 6-phosphate
CC       and fructose 6-phosphate. {ECO:0000269|PubMed:10648518}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-arabino-hex-3-ulose 6-phosphate = beta-D-fructose 6-
CC         phosphate; Xref=Rhea:RHEA:25900, ChEBI:CHEBI:57634,
CC         ChEBI:CHEBI:58542; EC=5.3.1.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde assimilation via RuMP
CC       pathway; D-fructose 6-phosphate from D-ribulose 5-phosphate and
CC       formaldehyde: step 2/2.
CC   -!- INDUCTION: By methanol or methylamine. {ECO:0000269|PubMed:10648518}.
CC   -!- SIMILARITY: Belongs to the SIS family. PHI subfamily. {ECO:0000305}.
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DR   EMBL; AB034913; BAA90545.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9LBW5; -.
DR   SMR; Q9LBW5; -.
DR   BRENDA; 5.3.1.27; 10297.
DR   UniPathway; UPA00294; UER00435.
DR   GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR   GO; GO:0043800; F:hexulose-6-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:1901135; P:carbohydrate derivative metabolic process; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019647; P:formaldehyde assimilation via ribulose monophosphate cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd05005; SIS_PHI; 1.
DR   InterPro; IPR017552; PHI/rmpB.
DR   InterPro; IPR001347; SIS_dom.
DR   InterPro; IPR046348; SIS_dom_sf.
DR   PANTHER; PTHR43443; PTHR43443; 1.
DR   Pfam; PF01380; SIS; 1.
DR   SUPFAM; SSF53697; SSF53697; 1.
DR   TIGRFAMs; TIGR03127; RuMP_HxlB; 1.
DR   PROSITE; PS51464; SIS; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Isomerase.
FT   CHAIN           1..199
FT                   /note="3-hexulose-6-phosphate isomerase"
FT                   /id="PRO_0000235171"
FT   DOMAIN          44..186
FT                   /note="SIS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00797"
FT   ACT_SITE        166
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   BINDING         62
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         101..106
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   199 AA;  20770 MW;  EBFEAADABBB2D786 CRC64;
     MTQAAEADGA VKVVGDDITN NLSLVRDEVA DTAAKVDPEQ VAVLARQIVQ PGRVFVAGAG
     RSGLVLRMAA MRLMHFGLTV HVAGDTTTPA ISAGDLLLVA SGSGTTSGVV KSAETAKKAG
     ARIAAFTTNP DSPLAGLADA VVIIPAAQKT DHGSHISRQY AGSLFEQVLF VVTEAVFQSL
     WDHTEVEAEE LWTRHANLE