ID   PHL1_LEPIC              Reviewed;         596 AA.
AC   Q72P44;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Sphingomyelinase C 1;
DE            EC=3.1.4.12;
DE   AltName: Full=Sphingomyelin phosphodiesterase 1;
DE            Short=SMase 1;
DE   Flags: Precursor;
GN   Name=sph1; OrderedLocusNames=LIC_12632;
OS   Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni
OS   (strain Fiocruz L1-130).
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=267671;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fiocruz L1-130;
RX   PubMed=15028702; DOI=10.1128/jb.186.7.2164-2172.2004;
RA   Nascimento A.L.T.O., Ko A.I., Martins E.A.L., Monteiro-Vitorello C.B.,
RA   Ho P.L., Haake D.A., Verjovski-Almeida S., Hartskeerl R.A., Marques M.V.,
RA   Oliveira M.C., Menck C.F.M., Leite L.C.C., Carrer H., Coutinho L.L.,
RA   Degrave W.M., Dellagostin O.A., El-Dorry H., Ferro E.S., Ferro M.I.T.,
RA   Furlan L.R., Gamberini M., Giglioti E.A., Goes-Neto A., Goldman G.H.,
RA   Goldman M.H.S., Harakava R., Jeronimo S.M.B., Junqueira-de-Azevedo I.L.M.,
RA   Kimura E.T., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Marino C.L.,
RA   Nunes L.R., de Oliveira R.C., Pereira G.G., Reis M.S., Schriefer A.,
RA   Siqueira W.J., Sommer P., Tsai S.M., Simpson A.J.G., Ferro J.A.,
RA   Camargo L.E.A., Kitajima J.P., Setubal J.C., Van Sluys M.A.;
RT   "Comparative genomics of two Leptospira interrogans serovars reveals novel
RT   insights into physiology and pathogenesis.";
RL   J. Bacteriol. 186:2164-2172(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a sphingomyelin + H2O = an N-acylsphing-4-enine + H(+) +
CC         phosphocholine; Xref=Rhea:RHEA:19253, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17636, ChEBI:CHEBI:52639,
CC         ChEBI:CHEBI:295975; EC=3.1.4.12;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
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DR   EMBL; AE016823; AAS71192.1; -; Genomic_DNA.
DR   RefSeq; WP_000629003.1; NC_005823.1.
DR   AlphaFoldDB; Q72P44; -.
DR   SMR; Q72P44; -.
DR   PaxDb; Q72P44; -.
DR   EnsemblBacteria; AAS71192; AAS71192; LIC_12632.
DR   GeneID; 61142510; -.
DR   KEGG; lic:LIC_12632; -.
DR   HOGENOM; CLU_489836_0_0_12; -.
DR   OMA; WPIEEKV; -.
DR   Proteomes; UP000007037; Chromosome I.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004767; F:sphingomyelin phosphodiesterase activity; IEA:UniProtKB-EC.
DR   CDD; cd09078; nSMase; 1.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR038772; SMPD2-like.
DR   InterPro; IPR017766; Sphingomyelinase/PLipase_C.
DR   PANTHER; PTHR12393; PTHR12393; 1.
DR   Pfam; PF03372; Exo_endo_phos; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   TIGRFAMs; TIGR03395; sphingomy; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Secreted; Signal.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000255"
FT   CHAIN           37..596
FT                   /note="Sphingomyelinase C 1"
FT                   /id="PRO_0000022049"
FT   REGION          63..118
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        66..118
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   596 AA;  68095 MW;  05A979DB24D16F6C CRC64;
     MITKRNIPCK KNWKYKKKSI SLTLITICYM FLFLTSCKPG KQNSINLLLL LLNTLDNKNV
     NEKIEDSTNT DPSSNVNEED ENSINANAND NAPSDSDSSN PRSPDKNPVN PTSPNSSSAD
     IGIKILSHSI FMAPTNLSSW GDLGQEERAQ RIASSSYIKN QDIIVFEGLS HNNAEKILLE
     KIRSEYPYQT NVVGRTKKGW NATLGAYTTS PMANGGVIIV SKWPIEEKVQ YIFNNSNCGQ
     DQYYNKGFAY VKINKDGKKF HVIGTQLQAR EPDCFNSGET IRKLQLNDIK SFIDSKDIPK
     DETVLITGDL NIIKGSNEYF DMISKLNVNE PRYVGVPFTL DTKTNALAAY YYEKEKPIYL
     DYILVSKLHA QPPVWQNLAY DPISNTTWKR SDGYTSYEFS DRYPVYGFIY ADSSTPTKSG
     HKRKYDQVSF QSTFNRKFIQ ADHNKKDGWL KADTRIKTDF TKFNLLQENV SESNPSCMNS
     GSVRIESSYY LNYYWNWFIG AASGDYGYYT KFNNGSDSLG IKNLDNGCLK DGSRVAFYDW
     DTIGGGYYYL TVWDKGSWKE HLFLWVQSFL SSREIFYLHL DSNPPKDWSK DLIYHH