PHL4_ARATH
ID PHL4_ARATH Reviewed; 397 AA.
AC Q8GXC2; Q9SK62;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Myb family transcription factor PHL4 {ECO:0000305};
DE AltName: Full=Protein PHR1-LIKE 4 {ECO:0000305};
GN Name=PHL4 {ECO:0000305};
GN OrderedLocusNames=At2g20400 {ECO:0000312|Araport:AT2G20400};
GN ORFNames=F11A3.5 {ECO:0000312|EMBL:AAD21748.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAC42929.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY.
RX PubMed=11511543; DOI=10.1101/gad.204401;
RA Rubio V., Linhares F., Solano R., Martin A.C., Iglesias J., Leyva A.,
RA Paz-Ares J.;
RT "A conserved MYB transcription factor involved in phosphate starvation
RT signaling both in vascular plants and in unicellular algae.";
RL Genes Dev. 15:2122-2133(2001).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22101548; DOI=10.1007/s00497-011-0178-8;
RA Renak D., Dupl'akova N., Honys D.;
RT "Wide-scale screening of T-DNA lines for transcription factor genes
RT affecting male gametophyte development in Arabidopsis.";
RL Sex. Plant Reprod. 25:39-60(2012).
CC -!- FUNCTION: Transcription factor involved in male gametophyte
CC development. {ECO:0000269|PubMed:22101548}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC -!- DISRUPTION PHENOTYPE: Separated male germ unit (MGU) with all nuclei
CC separated from each other making a wide triangular figure.
CC {ECO:0000269|PubMed:22101548}.
CC -!- SIMILARITY: Belongs to the MYB-CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21748.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006569; AAD21748.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07003.1; -; Genomic_DNA.
DR EMBL; AK118313; BAC42929.1; -; mRNA.
DR EMBL; BT006119; AAP04104.1; -; mRNA.
DR PIR; G84588; G84588.
DR RefSeq; NP_179630.2; NM_127599.3.
DR AlphaFoldDB; Q8GXC2; -.
DR SMR; Q8GXC2; -.
DR STRING; 3702.AT2G20400.1; -.
DR iPTMnet; Q8GXC2; -.
DR PaxDb; Q8GXC2; -.
DR PRIDE; Q8GXC2; -.
DR ProteomicsDB; 236747; -.
DR EnsemblPlants; AT2G20400.1; AT2G20400.1; AT2G20400.
DR GeneID; 816559; -.
DR Gramene; AT2G20400.1; AT2G20400.1; AT2G20400.
DR KEGG; ath:AT2G20400; -.
DR Araport; AT2G20400; -.
DR TAIR; locus:2038957; AT2G20400.
DR eggNOG; ENOG502QXMH; Eukaryota.
DR HOGENOM; CLU_044541_2_0_1; -.
DR InParanoid; Q8GXC2; -.
DR PhylomeDB; Q8GXC2; -.
DR PRO; PR:Q8GXC2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GXC2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR025756; Myb_CC_LHEQLE.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR InterPro; IPR044848; PHR1-like.
DR PANTHER; PTHR31314; PTHR31314; 1.
DR Pfam; PF14379; Myb_CC_LHEQLE; 1.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; DNA-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..397
FT /note="Myb family transcription factor PHL4"
FT /id="PRO_0000436861"
FT DOMAIN 228..288
FT /note="HTH myb-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 259..284
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 319..339
FT /note="Coiled coil"
FT /evidence="ECO:0000305"
FT REGION 359..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 332..337
FT /note="LHEQLE"
FT /evidence="ECO:0000305"
FT COMPBIAS 377..397
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 387
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q94CL7"
SQ SEQUENCE 397 AA; 44112 MW; CF58A7929A66CA4D CRC64;
MIPNDDDDAN SMKNYPLNDD DANSMKNYPL NDDDANSMEN YPLRSIPTEL SHTCSLIPPS
LPNPSEAAAD MSFNSELNQI MARPCDMLPA NGGAVGHNPF LEPGFNCPET TDWIPSPLPH
IYFPSGSPNL IMEDGVIDEI HKQSDLPLWY DDLITTDEDP LMSSILGDLL LDTNFNSASK
VQQPSMQSQI QQPQAVLQQP SSCVELRPLD RTVSSNSNNN SNSNNAAAAA KGRMRWTPEL
HEVFVDAVNQ LGGSNEATPK GVLKHMKVEG LTIFHVKSHL QKYRTAKYIP VPSEGSPEAR
LTPLEQITSD DTKRGIDITE TLRIQMEHQK KLHEQLESLR TMQLRIEEQG KALLMMIEKQ
NMGFGGPEQG EKTSAKTPEN GSEESESPRP KRPRNEE
