PHLB1_MOUSE
ID PHLB1_MOUSE Reviewed; 1371 AA.
AC Q6PDH0; Q80TV2;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Pleckstrin homology-like domain family B member 1;
DE AltName: Full=Protein LL5-alpha;
GN Name=Phldb1; Synonyms=Kiaa0638, Ll5a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 64-1371 (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [3]
RP IDENTIFICATION OF THE GENE.
RX PubMed=14532993;
RA Katoh M., Katoh M.;
RT "Identification and characterization of human LL5A gene and mouse Ll5a gene
RT in silico.";
RL Int. J. Oncol. 23:1477-1483(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-382 AND SER-520, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51; SER-223; SER-472;
RP SER-503; SER-520; SER-522; THR-524; SER-535; SER-541; SER-553; SER-557;
RP SER-580; SER-585 AND SER-1022, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [8]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-131 AND ARG-514, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PDH0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PDH0-2; Sequence=VSP_016741, VSP_016742, VSP_016743;
CC -!- DOMAIN: The PH domain mediates the binding to phosphoinositides.
CC {ECO:0000250}.
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DR EMBL; BC058712; AAH58712.1; -; mRNA.
DR EMBL; AK122336; BAC65618.1; -; mRNA.
DR CCDS; CCDS23118.1; -. [Q6PDH0-1]
DR RefSeq; NP_705765.3; NM_153537.4. [Q6PDH0-1]
DR AlphaFoldDB; Q6PDH0; -.
DR SMR; Q6PDH0; -.
DR BioGRID; 221937; 6.
DR STRING; 10090.ENSMUSP00000034611; -.
DR iPTMnet; Q6PDH0; -.
DR PhosphoSitePlus; Q6PDH0; -.
DR jPOST; Q6PDH0; -.
DR MaxQB; Q6PDH0; -.
DR PaxDb; Q6PDH0; -.
DR PeptideAtlas; Q6PDH0; -.
DR PRIDE; Q6PDH0; -.
DR ProteomicsDB; 289742; -. [Q6PDH0-1]
DR ProteomicsDB; 289743; -. [Q6PDH0-2]
DR Antibodypedia; 45815; 72 antibodies from 14 providers.
DR DNASU; 102693; -.
DR Ensembl; ENSMUST00000034611; ENSMUSP00000034611; ENSMUSG00000048537. [Q6PDH0-1]
DR GeneID; 102693; -.
DR KEGG; mmu:102693; -.
DR UCSC; uc009peh.2; mouse. [Q6PDH0-1]
DR CTD; 23187; -.
DR MGI; MGI:2143230; Phldb1.
DR VEuPathDB; HostDB:ENSMUSG00000048537; -.
DR eggNOG; ENOG502QPZY; Eukaryota.
DR GeneTree; ENSGT00940000155231; -.
DR InParanoid; Q6PDH0; -.
DR OMA; QEYVTLE; -.
DR OrthoDB; 70229at2759; -.
DR TreeFam; TF329165; -.
DR BioGRID-ORCS; 102693; 6 hits in 71 CRISPR screens.
DR ChiTaRS; Phldb1; mouse.
DR PRO; PR:Q6PDH0; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q6PDH0; protein.
DR Bgee; ENSMUSG00000048537; Expressed in saccule of membranous labyrinth and 256 other tissues.
DR ExpressionAtlas; Q6PDH0; baseline and differential.
DR Genevisible; Q6PDH0; MM.
DR GO; GO:0045180; C:basal cortex; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0045171; C:intercellular bridge; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:1904261; P:positive regulation of basement membrane assembly involved in embryonic body morphogenesis; ISO:MGI.
DR GO; GO:0010717; P:regulation of epithelial to mesenchymal transition; ISO:MGI.
DR GO; GO:0010470; P:regulation of gastrulation; ISO:MGI.
DR GO; GO:0070507; P:regulation of microtubule cytoskeleton organization; ISO:MGI.
DR CDD; cd00060; FHA; 1.
DR CDD; cd14673; PH_PHLDB1_2; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR000253; FHA_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR037810; PHLDB1/2/3_PH.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Methylation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1371
FT /note="Pleckstrin homology-like domain family B member 1"
FT /id="PRO_0000053892"
FT DOMAIN 64..125
FT /note="FHA"
FT DOMAIN 1261..1364
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 153..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 368..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 672..714
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 942..1020
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1124..1143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 688..798
FT /evidence="ECO:0000255"
FT COILED 1150..1216
FT /evidence="ECO:0000255"
FT COMPBIAS 158..182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..382
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 541..563
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 682..714
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 982..1020
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 51
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 131
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 192
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 220
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 223
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 325
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 335
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 382
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT MOD_RES 405
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 431
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 445
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 463
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 472
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 491
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 503
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 514
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 520
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 522
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 524
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 535
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 541
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 553
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 557
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 565
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 580
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 585
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 683
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 976
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86UU1"
FT MOD_RES 1022
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 917
FT /note="E -> EYVTLEQLRVVWGTPPMPPSPSPGLPSWASASQDLAPITCLPPMLPS
FT SFASITRSSK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_016741"
FT VAR_SEQ 1174
FT /note="R -> RVRLTGARRQQV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_016742"
FT VAR_SEQ 1325
FT /note="K -> KKRFFHFTMVTE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_016743"
FT CONFLICT 402
FT /note="R -> H (in Ref. 2; BAC65618)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1371 AA; 150070 MW; E0E5204FF8C3C8C5 CRC64;
MDPLNRSQLG PGCKTQAVVQ KGPLDLIETG QGLKVQTDKP HLVSLGSGRL STAITLLPLE
EGRTVIGSAA RDISLQGPGL APEHCYIENL RGTLTLYPCG NACTIDGLPV RQPTRLTQGC
MLCLGQSTFL RFNHPAEAKW MKSMIPAGVR APGPTYNPGS AESESLVNGN HTAQPATRAP
SACASHSSLV SSIEKDLQEI MDSLVLEEPG AAGKKPAATS PLSPMANGGR YLLSPPTSPG
AMSVGSSYEN TSPAFSPLSS PASSGSCASH SPSGQEPGPS VPPLVPARSS SYHLALQPPQ
SRPSGSRSSD SPRLGRKGGH ERPPSPGLRG LLTDSPAATV LAEARRTTES PRLGGQLPVV
AISLSEYPSS GARSQPASIP GSPKFQSPVP APRNKIGTLQ DRPPSPFREP PGTERVLTSS
PSRQLVGRTF SDGLAATRTL QPPESPRLGR RGLDSMRELP PLSPSLSRRA LSPLPARTAP
DPKLSREVAE SPRPRRWAAH GTSPEDFSLT LGARGRRTRS PSPTLGESLA PRKGSFSGRL
SPAYSLGSLT GASPRQSPRA QRKLSSGDLR VPIPRERKNS ITEISDNEDE LLEYHRRQRQ
ERLREQEMER LERQRLETIL NLCAEYSRAD GGPETGELPS IGEATAALAL AGRRPSRGLA
GAIVVSGRCG EESGGASQRL WESMERSDEE NLKEECSSTE STQQEHEDAP GAKHQGEVLA
VEEERAQVLG RVEQLKIRVK ELEQQLQEAA REAEMERALL QGEREAERAS LQKEQRAVDQ
LQEKLVALET GIQKDRDKEA DALETETKLF EDLEFQQLER ESRVEEEREL AGQGLLRSKA
ELLRSVSKRK ERLAVLDSQA GQIRAQAVQE SERLAREKNA ALQLLQKEKE KLNVLERRYH
SLTGGRPFPK TTSTLKEMEK LLLPAVDLEQ WYQELMSGLG TGLAAASPRS SPPPLPAKAS
RQLQVYRSKM DSDAASPLPR TRSGPLPSSS GSSSSSSQLS VATLGRSPSP KSALLAQNGT
SSLPRNLAAT LQDIETKRQL ALQQKGHQVI EEQRRRLAEL KQKAAAEAQC QWDALHGAGP
FSAGPSGFPA LMHHSILHHL PAGRERGEEG EHAYDTLSLE SSDSMETSIS TGGNSACSPD
NMSSASGLDM GKIEEMEKML KEAHAEKSRL MESREREMEL RRQALEEERR RREQVERRLQ
SESARRQQLV EKEVKLREKQ FSQARPLTRY LPNRKEDFDL KTHIESSGHG VDTCLHVVLS
SKVCRGYLIK MGGKIKSWKK RWFVFDRLKR TLSYYVDKHE TKLKGVIYFQ AIEEVYYDHL
RSAAKSPNPA LTFCVKTHDR LYYMVAPSAE AMRIWMDVIV TGAEGYTQFM N
