PHLG_PSEFL
ID PHLG_PSEFL Reviewed; 293 AA.
AC A0A2K9M484;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 25-APR-2018, sequence version 1.
DT 03-AUG-2022, entry version 10.
DE RecName: Full=2,4-diacetylphloroglucinol hydrolase {ECO:0000305};
DE Short=DAPG hydrolase {ECO:0000305};
DE EC=3.7.1.24 {ECO:0000269|PubMed:32152338};
GN Name=phlG {ECO:0000303|PubMed:32152338};
GN ORFNames=C0J56_17400 {ECO:0000312|EMBL:AUM70470.1};
OS Pseudomonas fluorescens.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=294;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2P24;
RA Wei H.-L., Zhang L.-Q.;
RT "The genome sequence of plant growth-promoting Pseudomonas fluorescens
RT 2P24.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=2P24;
RX PubMed=32152338; DOI=10.1038/s41598-020-60555-9;
RA Zhao M.M., Lyu N., Wang D., Wu X.G., Zhao Y.Z., Zhang L.Q., Zhou H.Y.;
RT "PhlG mediates the conversion of DAPG to MAPG in Pseudomonas fluorescens
RT 2P24.";
RL Sci. Rep. 10:4296-4296(2020).
CC -!- FUNCTION: Hydrolase that specifically degrades the potent antimicrobial
CC compound 2,4-diacetylphloroglucinol (DAPG) to equimolar amounts of
CC mildly toxic monoacetylphloroglucinol (MAPG) and acetate.
CC {ECO:0000269|PubMed:32152338}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2,4-diacetylphloroglucinol + H2O = 2-acetylphloroglucinol +
CC acetate; Xref=Rhea:RHEA:59184, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:64344, ChEBI:CHEBI:140662; EC=3.7.1.24;
CC Evidence={ECO:0000269|PubMed:32152338};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:Q4K423};
CC -!- INDUCTION: Expression is positively regulated by GacS and negatively
CC regulated by RsmE. {ECO:0000269|PubMed:32152338}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene slows down but does not
CC eliminate degradation of DAPG. Deletion does not alter the ability of
CC the bacterium to colonise plants or promote plant growth.
CC {ECO:0000269|PubMed:32152338}.
CC -!- SIMILARITY: Belongs to the DAPG/phloretin hydrolase family.
CC {ECO:0000305}.
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DR EMBL; CP025542; AUM70470.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2K9M484; -.
DR SMR; A0A2K9M484; -.
DR EnsemblBacteria; AUM70470; AUM70470; C0J56_17400.
DR Proteomes; UP000235160; Chromosome.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR041526; DAPG_hydrolase.
DR Pfam; PF18089; DAPG_hydrolase; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Metal-binding; Zinc.
FT CHAIN 1..293
FT /note="2,4-diacetylphloroglucinol hydrolase"
FT /id="PRO_0000450526"
FT BINDING 129
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q4K423"
FT BINDING 160
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q4K423"
FT BINDING 270
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q4K423"
FT BINDING 274
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q4K423"
SQ SEQUENCE 293 AA; 33385 MW; A691D5680CC07A0F CRC64;
MEARVMTPFT YFSLPMQKQF LANQQAVQGK PYAEFFRSKI SVPLSAVEKI QQGPMPLADT
LTPSIEDLNR MLAADFISEE AGYALLPGPM AYVQSRKFFP NCTAEMLKWW FMWHPLEAER
YTLWFPYAHV ENPCVHHERL SDTTLSFEES LYGNTFCASE YVGDRLMHLH IHFRDPCELG
FCPDLYRESK IDGSVSALMS LAHEPQVPVS LMAHLFKECP EGLYLTSRYW VGSHPAMQRF
PGAERAAQLL EESGLGEVEL ETLAYEFAVH DMCEFNHLAS ILPSLHAQFS GAK
