PHLP1_DICDI
ID PHLP1_DICDI Reviewed; 316 AA.
AC Q71A39; Q54CH7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Phosducin-like protein 1;
GN Name=phlp1; ORFNames=DDB_G0292850;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND THIOREDOXIN FOLD REGION.
RX PubMed=14517243; DOI=10.1093/emboj/cdg508;
RA Blaauw M., Knol J.C., Kortholt A., Roelofs J., Ruchira X., Postma M.,
RA Visser A.J.W.G., van Haastert P.J.M.;
RT "Phosducin-like proteins in Dictyostelium discoideum: implications for the
RT phosducin family of proteins.";
RL EMBO J. 22:5047-5057(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16135826; DOI=10.1128/mcb.25.18.8393-8400.2005;
RA Knol J.C., Engel R., Blaauw M., Visser A.J.W.G., van Haastert P.J.M.;
RT "The phosducin-like protein PhLP1 is essential for Gbetagamma dimer
RT formation in Dictyostelium discoideum.";
RL Mol. Cell. Biol. 25:8393-8400(2005).
CC -!- FUNCTION: Required for normal chemotaxis in response to cAMP and
CC folate. Required for the heterodimerization of the G protein beta and
CC gamma subunits gpbA and gpgA, which is itself thought to be necessary
CC for prenylation of the gamma subunit gpgA and its association with
CC plasma membranes. {ECO:0000269|PubMed:14517243,
CC ECO:0000269|PubMed:16135826}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16135826}.
CC -!- SIMILARITY: Belongs to the phosducin family. {ECO:0000305}.
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DR EMBL; AF540058; AAQ11192.1; -; mRNA.
DR EMBL; AAFI02000197; EAL60974.1; -; Genomic_DNA.
DR RefSeq; XP_629439.1; XM_629437.1.
DR AlphaFoldDB; Q71A39; -.
DR SMR; Q71A39; -.
DR STRING; 44689.DDB0201654; -.
DR PaxDb; Q71A39; -.
DR EnsemblProtists; EAL60974; EAL60974; DDB_G0292850.
DR GeneID; 8628958; -.
DR KEGG; ddi:DDB_G0292850; -.
DR dictyBase; DDB_G0292850; phlp1.
DR eggNOG; KOG3170; Eukaryota.
DR HOGENOM; CLU_881170_0_0_1; -.
DR InParanoid; Q71A39; -.
DR OMA; IENEHHL; -.
DR PhylomeDB; Q71A39; -.
DR PRO; PR:Q71A39; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0043326; P:chemotaxis to folate; IMP:dictyBase.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; IMP:dictyBase.
DR GO; GO:0043497; P:regulation of protein heterodimerization activity; IMP:dictyBase.
DR GO; GO:0031399; P:regulation of protein modification process; IMP:dictyBase.
DR CDD; cd02987; Phd_like_Phd; 1.
DR Gene3D; 1.10.168.10; -; 1.
DR InterPro; IPR001200; Phosducin.
DR InterPro; IPR023196; Phosducin_N_dom_sf.
DR InterPro; IPR024253; Phosducin_thioredoxin-like_dom.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR Pfam; PF02114; Phosducin; 1.
DR PRINTS; PR00677; PHOSDUCIN.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 2: Evidence at transcript level;
KW Chaperone; Chemotaxis; Coiled coil; Cytoplasm; Reference proteome;
KW Sensory transduction.
FT CHAIN 1..316
FT /note="Phosducin-like protein 1"
FT /id="PRO_0000326487"
FT REGION 1..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..316
FT /note="Thioredoxin fold"
FT REGION 293..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 102..156
FT /evidence="ECO:0000255"
FT COMPBIAS 13..51
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..316
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 316 AA; 36853 MW; 3E46F17A4695E6E1 CRC64;
MEQNILNSIL DKFGDGDQER SDIRHNDSGD ENDNHSDHEG NHGNNECCEG NEDGDKEYEV
DVEDMTDEQY AQFIQEQQEP KIKSGGNTGV KGVLSDYAEH REKQKQKYLQ KKYETQKMLE
KMCFTTRDQP PPTEEENQLD SDDDDLERIR KARMEQWKSK QQITSDVKKP EKKVFGYFKQ
IDSSQYIHEI DNEPPNVFVI IHLFQNYIPE CVLLNQQLGQ LAVKYRYIKF LKILSKEAKE
NYHDEALPSL LVYIGGKLLV SFVPLTEELG RNFDQEDLEL LLSSYDIIPN PMKAKNSNWE
TSLSRKRPES DDDNDD
