PHL_ARATH
ID PHL_ARATH Reviewed; 1325 AA.
AC F4IDB2; Q9C9D6; Q9C9D7;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Protein PHYTOCHROME-DEPENDENT LATE-FLOWERING {ECO:0000303|PubMed:24127609};
GN Name=PHL {ECO:0000303|PubMed:24127609};
GN OrderedLocusNames=At1g72390 {ECO:0000312|Araport:AT1G72390};
GN ORFNames=T10D10.14 {ECO:0000312|EMBL:AAG52583.1},
GN T10D10.15 {ECO:0000312|EMBL:AAG52585.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3] {ECO:0007744|PubMed:19376835}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ALA-1026, INTERACTION WITH
RP PHYB AND CO, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=24127609; DOI=10.1073/pnas.1310631110;
RA Endo M., Tanigawa Y., Murakami T., Araki T., Nagatani A.;
RT "PHYTOCHROME-DEPENDENT LATE-FLOWERING accelerates flowering through
RT physical interactions with phytochrome B and CONSTANS.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:18017-18022(2013).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND REPRESSION BY WHITE LIGHT.
RX PubMed=24614229; DOI=10.4161/psb.28118;
RA Endo M., Kudo D., Koto T., Shimizu H., Araki T.;
RT "Light-dependent destabilization of PHL in Arabidopsis.";
RL Plant Signal. Behav. 9:E28118-E28118(2014).
CC -!- FUNCTION: Triggers photoperiod-monitored flowering by repressing PHYB-
CC dependent flowering negative regulation, probably through physical
CC interactions with PHYB and CO. {ECO:0000269|PubMed:24127609,
CC ECO:0000269|PubMed:24614229}.
CC -!- SUBUNIT: Component of a red light-dependent nuclear complex made of
CC PHL, PHYB and CO. Interacts directly with PHYB and CO; CO binding
CC requires the presence of PHYB. {ECO:0000269|PubMed:24127609}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24127609}. Nucleus,
CC nuclear body {ECO:0000269|PubMed:24127609}. Cytoplasmic granule
CC {ECO:0000269|PubMed:24127609}. Cytoplasm {ECO:0000269|PubMed:24127609}.
CC Note=Circadian clock-regulated subcellular localization. During the
CC night, mainly present in the cytoplasm. During the day, moves and
CC accumulates progressively in the nuclei. {ECO:0000269|PubMed:24127609}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in cotyledons and leaves, both in
CC mesophyll and vasculature cells. Also present in roots, hypocotyls and
CC shoot apices. {ECO:0000269|PubMed:24127609}.
CC -!- INDUCTION: Repressed post-transcriptionally by white light (at protein
CC level). {ECO:0000269|PubMed:24614229}.
CC -!- DISRUPTION PHENOTYPE: Late flowering, especially under long-day
CC conditions (PubMed:24127609, PubMed:24614229). Reduced expression of FT
CC and SOC1 (PubMed:24127609). {ECO:0000269|PubMed:24127609,
CC ECO:0000269|PubMed:24614229}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52583.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG52585.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC016529; AAG52583.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC016529; AAG52585.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35315.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59278.1; -; Genomic_DNA.
DR PIR; G96747; G96747.
DR PIR; H96747; H96747.
DR RefSeq; NP_001321648.1; NM_001334526.1.
DR RefSeq; NP_177383.5; NM_105898.7.
DR AlphaFoldDB; F4IDB2; -.
DR STRING; 3702.AT1G72390.1; -.
DR iPTMnet; F4IDB2; -.
DR PaxDb; F4IDB2; -.
DR PRIDE; F4IDB2; -.
DR ProteomicsDB; 236350; -.
DR EnsemblPlants; AT1G72390.1; AT1G72390.1; AT1G72390.
DR EnsemblPlants; AT1G72390.2; AT1G72390.2; AT1G72390.
DR GeneID; 843571; -.
DR Gramene; AT1G72390.1; AT1G72390.1; AT1G72390.
DR Gramene; AT1G72390.2; AT1G72390.2; AT1G72390.
DR KEGG; ath:AT1G72390; -.
DR Araport; AT1G72390; -.
DR TAIR; locus:2193977; AT1G72390.
DR eggNOG; ENOG502SE7N; Eukaryota.
DR HOGENOM; CLU_006336_0_0_1; -.
DR InParanoid; F4IDB2; -.
DR OMA; PEHEVSF; -.
DR OrthoDB; 145203at2759; -.
DR PRO; PR:F4IDB2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4IDB2; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016604; C:nuclear body; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000124; C:SAGA complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0048574; P:long-day photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009416; P:response to light stimulus; IEP:UniProtKB.
DR InterPro; IPR021950; Spt20.
DR PANTHER; PTHR13526; PTHR13526; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Flowering; Nucleus; Receptor; Reference proteome.
FT CHAIN 1..1325
FT /note="Protein PHYTOCHROME-DEPENDENT LATE-FLOWERING"
FT /id="PRO_0000445019"
FT REGION 313..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 462..558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 593..616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 852..875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1160..1325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..332
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..516
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..558
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 859..875
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1026
FT /note="A->V: In phl-2; late flowering, especially under
FT long-day conditions."
FT /evidence="ECO:0000269|PubMed:24127609"
SQ SEQUENCE 1325 AA; 143310 MW; E6F547F548CF548B CRC64;
MGVSFKISKV GRKFRPKIST ELATPDSPKA IVLSGKPKAT DDSNIGDVSG FSKPSLPDIS
PDHEVSFILS LYPNGYSIGK TSEAMQQISF RDVPKVLHPY DRAAEGLLSA IEAGRLPGDI
LEDIPCKFVD GVVICEVHDY RKHTSSQVSP VINKLRLKMS LENVVKDIPS MSDNSWTYGD
LMEVESRILK ALQPELCLDP LPRLDRLSKN PLTAKLDLSL STLRRKRLRQ MAEVTVMSQN
KIQGKKVCID RLPESSERGN LPGHLIMQQT NNNQAIQNLG TNMLAGLRSQ PLQDAPNSSL
ALVPPQQQRY MGIGSTRNTQ DQGSNSVSVS GASPGGLDAM LPYGSDSMNP GTSFHRKRES
QEGQMSSMPG LNKRTRVSHM GPDGVPQQQL GQRMDGLHGS DTNWKNTLLQ HQDMLGRSIQ
YPNTSIQRFS PHQMEGVMNQ EGGPMQFPAS QQGGMKYTSK EEPFETGKID GGTRNNIPGV
GSDANDLDPR IQSRMPHNAF IRSNFPQTSW NVNPGQQIEK EPKKEEQFSR RISAQSPRLS
AGGPPQSPLS SKSGEFSGGS MGTHYGAVAA AQKDKAVTSI PAIGATQSVG SSANEAMQQR
QHQAQMAAKR RTNSLPKTQV ISTVGSPVSV NTISVPVNAR SPSVGPQTLG DHAILDRFSK
IERVAARYQL NCKKHKVDEY SRRPRVYAKQ PLTVCLSNLS NEEVFKDEDE ALSKSIFGGS
MNTYKTRVIH FGQMERVMQG SVPSFIPRNR TRLVMSEKAV DGTVAWYQGD VDEGDVFQAE
DFLLALPNTH IADLLATQFK SLMAREGYMI EEHIMAKPNR GDTGPISSHP NSAGGYPRGY
SANDMQQYGD AVAGQASGEA SKHGNTGNTP NNSTQNILAN ARMVPPTNSQ ALQMSQGLLS
GVSMPMQPQQ LDPQQSALLS SHSQQKNQQS MFTQQQHPQM QRPSMILPTN PLSAINSMSQ
SSGMQPGGQM ANKYSPLQLQ MLQQQQQAAV QKKIMMGLGS GVGMGMGMGM GMGMGSMGNS
IAGLGALGNQ LNMAGRGMGG TGISSSMSVP GIGNMGQNPM NLNPASNLNA ISQQLRSGAL
TPQQNALFTQ IRMGMANRGG VMGAPQTGIS GVSGTRQMHP SSAGLSMLDQ NRANLQRAAA
MGNMGPPKLM PGMMNLYMNQ QQQQQQLQQQ PQQQQLQHQQ QLQQPMSQPS QQLAQSPQQQ
QQLQQHEQPQ QAQQQQQATA SPLQSVLSPP QVGSPSAGIT QQQLQQSSPQ QMSQRTPMSP
QQVNQRTPMS PQISSGAMHP MSTSNLEGCP ASPQLSSQTM GSVGSITNSP MELQGPKNNS
AGNNS
