ID   PHL_LEPIR               Reviewed;         556 AA.
AC   P17627;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Sphingomyelinase C;
DE            EC=3.1.4.12;
DE   AltName: Full=Sphingomyelin phosphodiesterase;
DE            Short=SMase;
DE   Flags: Precursor;
GN   Name=sph;
OS   Leptospira interrogans.
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=173;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sponselee / Serogroup Sejroe / Serovar hardjo;
RX   PubMed=2163985; DOI=10.1128/iai.58.7.2177-2185.1990;
RA   Segers R.P.A.M., van der Drift A., de Nijs A., Corcione P.,
RA   van der Zeijst B.A.M., Gaastra W.;
RT   "Molecular analysis of a sphingomyelinase C gene from Leptospira
RT   interrogans serovar hardjo.";
RL   Infect. Immun. 58:2177-2185(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a sphingomyelin + H2O = an N-acylsphing-4-enine + H(+) +
CC         phosphocholine; Xref=Rhea:RHEA:19253, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17636, ChEBI:CHEBI:52639,
CC         ChEBI:CHEBI:295975; EC=3.1.4.12;
CC   -!- SUBCELLULAR LOCATION: Secreted.
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DR   EMBL; X52176; CAA36424.1; -; Genomic_DNA.
DR   PIR; S22634; S22634.
DR   AlphaFoldDB; P17627; -.
DR   SMR; P17627; -.
DR   PRIDE; P17627; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004767; F:sphingomyelin phosphodiesterase activity; IEA:UniProtKB-EC.
DR   CDD; cd09078; nSMase; 1.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR038772; SMPD2-like.
DR   InterPro; IPR017766; Sphingomyelinase/PLipase_C.
DR   PANTHER; PTHR12393; PTHR12393; 1.
DR   Pfam; PF03372; Exo_endo_phos; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   TIGRFAMs; TIGR03395; sphingomy; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..556
FT                   /note="Sphingomyelinase C"
FT                   /id="PRO_0000022052"
SQ   SEQUENCE   556 AA;  63269 MW;  6D0EACDB9A5CD6FD CRC64;
     MRIKKYTKVR LLVNCCLLLF FLIDCGADRQ SLYKDLLASL IYISDNKNIG STNSDLTGSG
     SVSSSPADAA PENSILANSI PENMGIKILT HNVFLLPKTL PGWGNWGQNE RAQRIVSSNY
     IQNQDVIVFD EAFDTDARKI LLDGVRSEYP YQTDVIGRTK KGWDATLGLY RTDAFTNGGV
     VIVSKWPIEE KIQHVFKEKG CGADVFSNKG FAYVRIDKNG RKFHIIGTHV QAQDSGCANL
     GVVSRVNQFN EIRDFIDSKK IPKNEMVLIA GDLNVIKGSR EYHQMLCILN VNNPKYVGVP
     FTWDTKTNEI AAFYYKKVEP AYLDYIFVSK SHFQPPIWQN LAYDPISAKT WTAKGYTSDE
     FSDHYPVYGF IYADSSTPTK SGRKRKYDRV SFVSVATGKK IQANSEKSNA WLKVNATTET
     DLTKFNLVQT NDPDSNPSCM KSGHVRIESS HSLNYFWNWW LGGGKGNYAY YPKFNDGSNR
     IQIINLDGGC LQDGSRVAFK DYDTISRRQY FLTVWEGGNW DKYLYLWRSH IGLREIFYLK
     LDSSPEMNWS KKLIYR