ID   PHMT_MYCTU              Reviewed;         270 AA.
AC   P9WIN3; L0TBC8; O08026; Q50464; Q798M4; Q7D6D5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=Rv2952;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   FUNCTION AS METHYLTRANSFERASE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15292265; DOI=10.1074/jbc.m406134200;
RA   Perez E., Constant P., Laval F., Lemassu A., Laneelle M.-A., Daffe M.,
RA   Guilhot C.;
RT   "Molecular dissection of the role of two methyltransferases in the
RT   biosynthesis of phenolglycolipids and phthiocerol dimycoserosate in the
RT   Mycobacterium tuberculosis complex.";
RL   J. Biol. Chem. 279:42584-42592(2004).
RN   [4]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Catalyzes the methylation of the lipid moiety of the
CC       intermediate compounds phthiotriol and glycosylated phenolphthiotriol
CC       dimycoserosates to form phthiocerol dimycocerosates (DIM A) and
CC       glycosylated phenolphthiocerol dimycocerosates (PGL).
CC       {ECO:0000269|PubMed:15292265}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; U00024; AAA50934.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP45756.1; -; Genomic_DNA.
DR   PIR; E70669; E70669.
DR   RefSeq; NP_217468.1; NC_000962.3.
DR   RefSeq; WP_003414900.1; NZ_NVQJ01000015.1.
DR   AlphaFoldDB; P9WIN3; -.
DR   SMR; P9WIN3; -.
DR   STRING; 83332.Rv2952; -.
DR   PaxDb; P9WIN3; -.
DR   DNASU; 887311; -.
DR   GeneID; 887311; -.
DR   KEGG; mtu:Rv2952; -.
DR   TubercuList; Rv2952; -.
DR   eggNOG; COG2226; Bacteria.
DR   OMA; EWILRAH; -.
DR   PhylomeDB; P9WIN3; -.
DR   BioCyc; MetaCyc:G185E-7206-MON; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0008168; F:methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0008171; F:O-methyltransferase activity; IDA:MTBBASE.
DR   GO; GO:0071770; P:DIM/DIP cell wall layer assembly; IDA:MTBBASE.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Lipid biosynthesis; Lipid metabolism; Methyltransferase;
KW   Reference proteome; Transferase.
FT   CHAIN           1..270
FT                   /note="Phthiotriol/phenolphthiotriol dimycocerosates
FT                   methyltransferase"
FT                   /id="PRO_0000305165"
SQ   SEQUENCE   270 AA;  30652 MW;  92116ADD4261EDE7 CRC64;
     MAFSRTHSLL ARAGSTSTYK RVWRYWYPLM TRGLGNDEIV FINWAYEEDP PMDLPLEASD
     EPNRAHINLY HRTATQVDLG GKQVLEVSCG HGGGASYLTR TLHPASYTGL DLNQAGIKLC
     KKRHRLPGLD FVRGDAENLP FDDESFDVVL NVEASHCYPH FRRFLAEVVR VLRPGGYFPY
     ADLRPNNEIA AWEADLAATP LRQLSQRQIN AEVLRGIGNN SQKSRDLVDR HLPAFLRFAG
     REFIGVQGTQ LSRYLEGGEL SYRMYCFTKD