PHNC_ECOLI
ID PHNC_ECOLI Reviewed; 262 AA.
AC P16677; Q2M6J8;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Phosphonates import ATP-binding protein PhnC {ECO:0000255|HAMAP-Rule:MF_01713};
DE EC=7.3.2.2 {ECO:0000255|HAMAP-Rule:MF_01713};
GN Name=phnC {ECO:0000255|HAMAP-Rule:MF_01713};
GN OrderedLocusNames=b4106, JW4067;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=B;
RX PubMed=2155230; DOI=10.1016/s0021-9258(19)39587-0;
RA Chen C.-M., Ye Q.-Z., Zhu Z., Wanner B.L., Walsh C.T.;
RT "Molecular biology of carbon-phosphorus bond cleavage. Cloning and
RT sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and
RT C-P lyase activity in Escherichia coli B.";
RL J. Biol. Chem. 265:4461-4471(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1840580; DOI=10.1128/jb.173.8.2665-2672.1991;
RA Makino K., Kim S.K., Shinagawa H., Amemura M., Nakata A.;
RT "Molecular analysis of the cryptic and functional phn operons for
RT phosphonate use in Escherichia coli K-12.";
RL J. Bacteriol. 173:2665-2672(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP FUNCTION.
RX PubMed=1846145; DOI=10.1128/jb.173.2.587-600.1991;
RA Metcalf W.W., Wanner B.L.;
RT "Involvement of the Escherichia coli phn (psiD) gene cluster in
RT assimilation of phosphorus in the form of phosphonates, phosphite, Pi
RT esters, and Pi.";
RL J. Bacteriol. 173:587-600(1991).
RN [7]
RP FUNCTION.
RX PubMed=8388873; DOI=10.1128/jb.175.11.3430-3442.1993;
RA Metcalf W.W., Wanner B.L.;
RT "Mutational analysis of an Escherichia coli fourteen-gene operon for
RT phosphonate degradation, using TnphoA' elements.";
RL J. Bacteriol. 175:3430-3442(1993).
CC -!- FUNCTION: Part of the ABC transporter complex PhnCDE involved in
CC phosphonates, phosphate esters, phosphite and phosphate import.
CC Responsible for energy coupling to the transport system.
CC {ECO:0000269|PubMed:1846145, ECO:0000269|PubMed:8388873}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + phosphonate(out) = ADP + H(+) + phosphate +
CC phosphonate(in); Xref=Rhea:RHEA:18065, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16215, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01713};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PhnC),
CC two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).
CC {ECO:0000255|HAMAP-Rule:MF_01713}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01713}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01713}.
CC -!- MISCELLANEOUS: Phosphonates utilization is cryptic in strain K12.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Phosphonates
CC importer (TC 3.A.1.9.1) family. {ECO:0000255|HAMAP-Rule:MF_01713}.
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DR EMBL; J05260; AAA24339.1; -; Genomic_DNA.
DR EMBL; D90227; BAA14262.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97005.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77067.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78108.1; -; Genomic_DNA.
DR PIR; A65220; A65220.
DR RefSeq; NP_418530.1; NC_000913.3.
DR RefSeq; WP_001193409.1; NZ_SSZK01000016.1.
DR AlphaFoldDB; P16677; -.
DR SMR; P16677; -.
DR IntAct; P16677; 1.
DR STRING; 511145.b4106; -.
DR TCDB; 3.A.1.9.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; P16677; -.
DR PRIDE; P16677; -.
DR EnsemblBacteria; AAC77067; AAC77067; b4106.
DR EnsemblBacteria; BAE78108; BAE78108; BAE78108.
DR GeneID; 948623; -.
DR KEGG; ecj:JW4067; -.
DR KEGG; eco:b4106; -.
DR PATRIC; fig|1411691.4.peg.2594; -.
DR EchoBASE; EB0707; -.
DR eggNOG; COG3638; Bacteria.
DR HOGENOM; CLU_000604_1_22_6; -.
DR InParanoid; P16677; -.
DR OMA; GRMPRWR; -.
DR PhylomeDB; P16677; -.
DR BioCyc; EcoCyc:PHNC-MON; -.
DR PRO; PR:P16677; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015416; F:ABC-type phosphonate transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR CDD; cd03256; ABC_PhnC_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR012693; ABC_transpr_PhnC.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02315; ABC_phnC; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51249; PHNC; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Phosphonate transport; Reference proteome; Translocase;
KW Transport.
FT CHAIN 1..262
FT /note="Phosphonates import ATP-binding protein PhnC"
FT /id="PRO_0000092706"
FT DOMAIN 5..253
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01713"
FT BINDING 37..44
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01713"
FT VARIANT 60
FT /note="V -> A (in strain: B)"
FT VARIANT 87
FT /note="H -> N (in strain: B)"
FT VARIANT 127
FT /note="G -> R (in strain: B)"
FT VARIANT 255
FT /note="V -> I (in strain: B)"
SQ SEQUENCE 262 AA; 29431 MW; 393EF8F30FD18D6C CRC64;
MQTIIRVEKL AKTFNQHQAL HAVDLNIHHG EMVALLGPSG SGKSTLLRHL SGLITGDKSV
GSHIELLGRT VQREGRLARD IRKSRAHTGY IFQQFNLVNR LSVLENVLIG ALGSTPFWRT
CFSWFTGEQK QRALQALTRV GMVHFAHQRV STLSGGQQQR VAIARALMQQ AKVILADEPI
ASLDPESARI VMDTLRDINQ NDGITVVVTL HQVDYALRYC ERIVALRQGH VFYDGSSQQF
DNERFDHLYR SINRVEENAK AA
