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PHNE1_ECOLI
ID   PHNE1_ECOLI             Reviewed;         206 AA.
AC   P0DP69; P16683; P76792; Q2M6K0; Q47479; Q47716;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2017, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Putative cryptic phosphonate transport system permease protein PhnE1;
GN   Name=phnE1; OrderedLocusNames=b4104, JW4065;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION OF MUTATION.
RC   STRAIN=K12 / W3110;
RX   PubMed=1840580; DOI=10.1128/jb.173.8.2665-2672.1991;
RA   Makino K., Kim S.K., Shinagawa H., Amemura M., Nakata A.;
RT   "Molecular analysis of the cryptic and functional phn operons for
RT   phosphonate use in Escherichia coli K-12.";
RL   J. Bacteriol. 173:2665-2672(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   CRYPTIC FUNCTION.
RX   PubMed=2155195; DOI=10.1128/jb.172.3.1186-1196.1990;
RA   Wanner B.L., Boline J.A.;
RT   "Mapping and molecular cloning of the phn (psiD) locus for phosphonate
RT   utilization in Escherichia coli.";
RL   J. Bacteriol. 172:1186-1196(1990).
CC   -!- FUNCTION: N-terminal fragment of the PhnE protein, part of a
CC       phosphonate usage operon that is cryptic in K12 strains. Growth of K12
CC       strains on phosphonate can be observed when it is used as the sole
CC       phosphorus source after a 60 hour lag period, suggesting the operon is
CC       activated (PubMed:2155195). An intact PhnE in strain B is (AC
CC       A0A140NFA3). Part of the binding-protein-dependent transport system for
CC       phosphonates; probably responsible for the translocation of the
CC       substrate across the membrane (Probable). {ECO:0000305|PubMed:2155195}.
CC   -!- SUBUNIT: If the reading frame is restored, the complex is composed of
CC       two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and
CC       a solute-binding protein (PhnD) (Probable). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- CAUTION: Could be the product of a pseudogene. In K12 strains the phnE
CC       gene has an 8-bp insertion, absent from the strain B gene, which causes
CC       a frameshift mutation (PubMed:1840580). In 8 K12 derivatives selected
CC       for their ability to grow on phosphonate this repeat is no longer
CC       present, which restores an intact PhnE open reading frame
CC       (PubMed:1840580). {ECO:0000269|PubMed:1840580}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA14264.1; Type=Erroneous initiation; Note=Truncated N-terminus.;
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DR   EMBL; D90227; BAA14264.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U14003; AAA97003.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78106.1; -; Genomic_DNA.
DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; F35718; F35718.
DR   AlphaFoldDB; P0DP69; -.
DR   EnsemblBacteria; BAE78106; BAE78106; BAE78106.
DR   HOGENOM; CLU_092815_0_0_6; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015716; P:organic phosphonate transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   SUPFAM; SSF161098; SSF161098; 1.
PE   5: Uncertain;
KW   Cell inner membrane; Cell membrane; Membrane; Phosphonate transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..206
FT                   /note="Putative cryptic phosphonate transport system
FT                   permease protein PhnE1"
FT                   /id="PRO_0000440701"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   206 AA;  22797 MW;  FCC94BB605706150 CRC64;
     MPDAVQAPYP AYRPEPNMQT ITIAPPKRSW FSLLSWAVVL AVLVVSWQGA EMAPLTLIKD
     GGNMATFAAD FFPPDFSQWQ DYLTEMAVTL QIAVWGTALA VVLSIPFGLM SAENLVPWWV
     YQPVRRLMDA CRAINEMVFA MLFVVAVGLG PFAGVLACWR CLSTPPACSP SCFPKRWKRL
     SPARWKAFAP PVPTSSKRSS TACCHR
 
 
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