PHNE2_ECOLI
ID PHNE2_ECOLI Reviewed; 122 AA.
AC P0DP70; P16683; P76792; Q2M6K0; Q47479; Q47716;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2017, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=Putative cryptic phosphonate transport system permease protein PhnE2;
GN Name=phnE; Synonyms=phnE2 {ECO:0000305}; OrderedLocusNames=b4103, JW4065;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110;
RX PubMed=1840580; DOI=10.1128/jb.173.8.2665-2672.1991;
RA Makino K., Kim S.K., Shinagawa H., Amemura M., Nakata A.;
RT "Molecular analysis of the cryptic and functional phn operons for
RT phosphonate use in Escherichia coli K-12.";
RL J. Bacteriol. 173:2665-2672(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CRYPTIC FUNCTION.
RX PubMed=2155195; DOI=10.1128/jb.172.3.1186-1196.1990;
RA Wanner B.L., Boline J.A.;
RT "Mapping and molecular cloning of the phn (psiD) locus for phosphonate
RT utilization in Escherichia coli.";
RL J. Bacteriol. 172:1186-1196(1990).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: C-terminal fragment of the PhnE protein, part of a
CC phosphonate usage operon that is cryptic in K12 strains. Growth of K12
CC strains on phosphonate can be observed when it is used as the sole
CC phosphorus source after a 60 hour lag period, suggesting the operon is
CC activated (PubMed:2155195). An intact PhnE in strain B is (AC
CC A0A140NFA3). Part of the binding-protein-dependent transport system for
CC phosphonates; probably responsible for the translocation of the
CC substrate across the membrane (Probable). {ECO:0000305|PubMed:2155195}.
CC -!- SUBUNIT: If the reading frame is restored, the complex is composed of
CC two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and
CC a solute-binding protein (PhnD) (Probable). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:15919996}.
CC Note=In strain K12 / MG1655 when overexpressed using vectors that
CC provide a promoter and ribosome binding site (PubMed:15919996).
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. In K12 strains the phnE
CC gene has an 8-bp insertion, absent from the strain B gene, which causes
CC a frameshift mutation (PubMed:1840580). In 8 K12 derivatives selected
CC for their ability to grow on phosphonate this repeat is no longer
CC present, which restores an intact PhnE open reading frame
CC (PubMed:1840580). {ECO:0000269|PubMed:1840580}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA97002.1; Type=Erroneous initiation; Note=Truncated N-terminus.;
CC Sequence=BAA14265.1; Type=Erroneous initiation; Note=Truncated N-terminus.;
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DR EMBL; D90227; BAA14265.1; ALT_INIT; Genomic_DNA.
DR EMBL; U14003; AAA97002.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; F35718; F35718.
DR AlphaFoldDB; P0DP70; -.
DR SMR; P0DP70; -.
DR EchoBASE; EB1260; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015716; P:organic phosphonate transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 5: Uncertain;
KW Cell inner membrane; Cell membrane; Membrane; Phosphonate transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..122
FT /note="Putative cryptic phosphonate transport system
FT permease protein PhnE2"
FT /id="PRO_0000060173"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT ProRule:PRU00441"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT ProRule:PRU00441"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT ProRule:PRU00441"
FT DOMAIN 1..114
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 122 AA; 13335 MW; 56E828CA20D327B4 CRC64;
MLALFIHTTG VLSKLLSEAV EAIEPGPVEG IRATGANKLE EILYGVLPQV MPLLISYSLY
RFESNVRSAT VVGMVGAGGI GVTLWEAIRG FQFQQTCALM VLIIVTVSLL DFLSQRLRKH
FI
