ID   PHNE_ECOBD              Reviewed;         259 AA.
AC   A0A140NFA3; P16683; P76792; Q2M6K0; Q47479; Q47716;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   11-MAY-2016, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Phosphonate transport system permease protein PhnE;
GN   Name=phnE; OrderedLocusNames=ECBD_3927;
OS   Escherichia coli (strain B / BL21-DE3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=469008;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROBABLE FUNCTION.
RC   STRAIN=B / BW11334;
RX   PubMed=2155230; DOI=10.1016/s0021-9258(19)39587-0;
RA   Chen C.-M., Ye Q.-Z., Zhu Z., Wanner B.L., Walsh C.T.;
RT   "Molecular biology of carbon-phosphorus bond cleavage. Cloning and
RT   sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and
RT   C-P lyase activity in Escherichia coli B.";
RL   J. Biol. Chem. 265:4461-4471(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B / BL21-DE3;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.;
RT   "Complete sequence of Escherichia coli BL21(DE3).";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of the binding-protein-dependent transport system for
CC       phosphonates; probably responsible for the translocation of the
CC       substrate across the membrane. {ECO:0000305|PubMed:2155230}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PhnC),
CC       two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P16683}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA24341.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; J05260; AAA24341.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP001665; ACT30913.1; -; Genomic_DNA.
DR   RefSeq; WP_001193500.1; NZ_CP053602.1.
DR   AlphaFoldDB; A0A140NFA3; -.
DR   SMR; A0A140NFA3; -.
DR   STRING; 469008.B21_03936; -.
DR   GeneID; 66671986; -.
DR   KEGG; ebd:ECBD_3927; -.
DR   eggNOG; COG3639; Bacteria.
DR   HOGENOM; CLU_064254_0_2_6; -.
DR   OMA; RFAIIGR; -.
DR   Proteomes; UP000002032; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015416; F:ABC-type phosphonate transporter activity; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005769; PhnE/PtxC.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR01097; PhnE; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..259
FT                   /note="Phosphonate transport system permease protein PhnE"
FT                   /id="PRO_0000440702"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00441"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00441"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00441"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00441"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT                   ProRule:PRU00441"
FT   DOMAIN          69..251
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   259 AA;  28384 MW;  E6438A442CC3A842 CRC64;
     MQTITIAPPK RSWFSLLSWA VVLAVLVVSW QGAEMAPLTL IKDGGNMATF AADFFPPDFS
     QWQDYLTEMA VTLQIAVWGT ALAVVLSIPF GLMSAENLVP WWVYQPVRRL MDACRAINEM
     VFAMLFVVAV GLGPFAGVLA LFIHTTGVLS KLLSEAVEAI EPGPVEGIRA TGANKLEEIL
     YGVLPQVMPL LISYSLYRFE SNVRSATVVG MVGAGGIGVT LWEAIRGFQF QQTCALMVLI
     IVTVSLLDFL SQRLRKHFI