PHNE_MYCS2
ID PHNE_MYCS2 Reviewed; 532 AA.
AC A0QQ68; I7G248;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Phosphate-import permease protein PhnE;
GN Name=phnE; OrderedLocusNames=MSMEG_0646, MSMEI_0630;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP INVOLVEMENT IN PHOSPHATE ASSIMILATION, AND GENE NAME.
RX PubMed=15758213; DOI=10.1099/mic.0.27624-0;
RA Tran S.L., Rao M., Simmers C., Gebhard S., Olsson K., Cook G.M.;
RT "Mutants of Mycobacterium smegmatis unable to grow at acidic pH in the
RT presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone.";
RL Microbiology 151:665-672(2005).
RN [5]
RP FUNCTION IN PHOSPHATE TRANSPORT, AND INDUCTION.
RX PubMed=17074913; DOI=10.1099/mic.0.29201-0;
RA Gebhard S., Tran S.L., Cook G.M.;
RT "The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-
RT transporter for phosphate.";
RL Microbiology 152:3453-3465(2006).
CC -!- FUNCTION: Part of the ABC transporter complex PhnCDE involved in
CC phosphate import. Responsible for the translocation of the substrate
CC across the membrane. {ECO:0000269|PubMed:17074913}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PhnC),
CC two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- INDUCTION: By phosphate-limited conditions, via derepression by PhnF,
CC and probably also via the two-component regulatory system senX3/regX3.
CC {ECO:0000269|PubMed:17074913}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. {ECO:0000305}.
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DR EMBL; CP000480; ABK75678.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP37111.1; -; Genomic_DNA.
DR RefSeq; WP_011727091.1; NZ_SIJM01000009.1.
DR RefSeq; YP_885056.1; NC_008596.1.
DR AlphaFoldDB; A0QQ68; -.
DR SMR; A0QQ68; -.
DR STRING; 246196.MSMEI_0630; -.
DR TCDB; 3.A.1.9.2; the atp-binding cassette (abc) superfamily.
DR EnsemblBacteria; ABK75678; ABK75678; MSMEG_0646.
DR EnsemblBacteria; AFP37111; AFP37111; MSMEI_0630.
DR GeneID; 66738824; -.
DR KEGG; msg:MSMEI_0630; -.
DR KEGG; msm:MSMEG_0646; -.
DR PATRIC; fig|246196.19.peg.642; -.
DR eggNOG; COG3639; Bacteria.
DR OMA; YRFECAL; -.
DR OrthoDB; 426495at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0015416; F:ABC-type phosphonate transporter activity; IEA:InterPro.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR CDD; cd06261; TM_PBP2; 2.
DR Gene3D; 1.10.3720.10; -; 2.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR005769; PhnE/PtxC.
DR Pfam; PF00528; BPD_transp_1; 2.
DR SUPFAM; SSF161098; SSF161098; 2.
DR TIGRFAMs; TIGR01097; PhnE; 2.
DR PROSITE; PS50928; ABC_TM1; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphate transport; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..532
FT /note="Phosphate-import permease protein PhnE"
FT /id="PRO_0000357469"
FT TRANSMEM 23..45
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 85..107
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 134..156
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 187..209
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 216..235
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 245..267
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 287..304
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 345..367
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 395..417
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 452..471
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 478..495
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 505..527
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 81..264
FT /note="ABC transmembrane type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 341..524
FT /note="ABC transmembrane type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 532 AA; 55928 MW; 411F7E36201A62A3 CRC64;
MTTEITRPPA PPSRPSESRK PSLPGLLHLV AIAAVLATIV SAWAIDFVPT ALIDGSDNIV
ALLQRMIPPR LDDPARIGML AVETLLMAVL GTTLAAIASV PLAFLAARNT TPHPAVQAVA
RAVITFCRAM PDLLFAVLFV RALGIGVLPG VLALALHSIG MLGKVFADAI EQTDAGPREA
VRSTGVGYFR ELLNAVVPQV VPSWIAMFVY RIDINLRMSV VLGFVGAGGI GFALQDALRG
LIYPRALGIV CVILVIIAGM ELLAIAIRRI LLDPSRSNPL RDRIARFGLS GVLVGSCVAA
FVLLKINPLA LFTWVFPSVG IFTRMVPPNF DALGVDLFTA AAQTVAIGVV ATAIGIALSI
PAGILAARNV SPHPALYWPA RAWILVVRAV PELILAVVFV AALGLGPIAG TCALAIGSIG
FLAKLVADAV EEIDPGPMEA VRSVGGGWWK TLFAAVLPQS MPALVGSSLY LFDVNVRTST
ILGIVGAGGV GYLLFESIRT LNFDVAGAIV IVIFVIVYAI ERLSGWIRSR LV
