ID   PHNF_ECOLI              Reviewed;         241 AA.
AC   P16684; Q2M6K1;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 159.
DE   RecName: Full=Probable transcriptional regulator PhnF;
GN   Name=phnF; OrderedLocusNames=b4102, JW4063;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B;
RX   PubMed=2155230; DOI=10.1016/s0021-9258(19)39587-0;
RA   Chen C.-M., Ye Q.-Z., Zhu Z., Wanner B.L., Walsh C.T.;
RT   "Molecular biology of carbon-phosphorus bond cleavage. Cloning and
RT   sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and
RT   C-P lyase activity in Escherichia coli B.";
RL   J. Biol. Chem. 265:4461-4471(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1840580; DOI=10.1128/jb.173.8.2665-2672.1991;
RA   Makino K., Kim S.K., Shinagawa H., Amemura M., Nakata A.;
RT   "Molecular analysis of the cryptic and functional phn operons for
RT   phosphonate use in Escherichia coli K-12.";
RL   J. Bacteriol. 173:2665-2672(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA   Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT   from 92.8 through 100 minutes.";
RL   Nucleic Acids Res. 23:2105-2119(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Belongs to an operon involved in alkylphosphonate uptake and
CC       C-P lyase. Exact function not known. By similarity could be a
CC       transcriptional regulator.
CC   -!- MISCELLANEOUS: The sequence shown is that of strains K12 and B.
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DR   EMBL; J05260; AAA24343.1; -; Genomic_DNA.
DR   EMBL; D90227; BAA14266.1; -; Genomic_DNA.
DR   EMBL; U14003; AAA97001.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC77063.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE78105.1; -; Genomic_DNA.
DR   PIR; G35718; G35718.
DR   RefSeq; NP_418526.1; NC_000913.3.
DR   RefSeq; WP_001295387.1; NZ_STEB01000014.1.
DR   PDB; 2FA1; X-ray; 1.70 A; A/B=85-241.
DR   PDBsum; 2FA1; -.
DR   AlphaFoldDB; P16684; -.
DR   SMR; P16684; -.
DR   BioGRID; 4263093; 10.
DR   IntAct; P16684; 2.
DR   STRING; 511145.b4102; -.
DR   PaxDb; P16684; -.
DR   PRIDE; P16684; -.
DR   EnsemblBacteria; AAC77063; AAC77063; b4102.
DR   EnsemblBacteria; BAE78105; BAE78105; BAE78105.
DR   GeneID; 58459677; -.
DR   GeneID; 948617; -.
DR   KEGG; ecj:JW4063; -.
DR   KEGG; eco:b4102; -.
DR   PATRIC; fig|1411691.4.peg.2598; -.
DR   EchoBASE; EB0709; -.
DR   eggNOG; COG2188; Bacteria.
DR   HOGENOM; CLU_063236_2_2_6; -.
DR   InParanoid; P16684; -.
DR   OMA; HRPDKFR; -.
DR   PhylomeDB; P16684; -.
DR   BioCyc; EcoCyc:EG10715-MON; -.
DR   EvolutionaryTrace; P16684; -.
DR   PRO; PR:P16684; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0015716; P:organic phosphonate transport; IEA:UniProtKB-KW.
DR   CDD; cd07377; WHTH_GntR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.40.1410.10; -; 1.
DR   InterPro; IPR028978; Chorismate_lyase_/UTRA_dom_sf.
DR   InterPro; IPR012702; CP_lyase_PhnF.
DR   InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR   InterPro; IPR011663; UTRA.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00392; GntR; 1.
DR   Pfam; PF07702; UTRA; 1.
DR   PRINTS; PR00035; HTHGNTR.
DR   SMART; SM00345; HTH_GNTR; 1.
DR   SMART; SM00866; UTRA; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF64288; SSF64288; 1.
DR   TIGRFAMs; TIGR02325; C_P_lyase_phnF; 1.
DR   PROSITE; PS50949; HTH_GNTR; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alkylphosphonate uptake; DNA-binding; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..241
FT                   /note="Probable transcriptional regulator PhnF"
FT                   /id="PRO_0000050667"
FT   DOMAIN          11..78
FT                   /note="HTH gntR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT   DNA_BIND        38..57
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT   STRAND          87..93
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          104..112
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   HELIX           115..121
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          127..138
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          141..151
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   HELIX           153..155
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   HELIX           156..159
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   HELIX           167..175
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          179..190
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   HELIX           194..199
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          206..216
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          223..231
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   TURN            232..234
FT                   /evidence="ECO:0007829|PDB:2FA1"
FT   STRAND          235..240
FT                   /evidence="ECO:0007829|PDB:2FA1"
SQ   SEQUENCE   241 AA;  27621 MW;  F1A24763A84D574A CRC64;
     MHLSTHPTSY PTRYQEIAAK LEQELRQHYR CGDYLPAEQQ LAARFEVNRH TLRRAIDQLV
     EKGWVQRRQG VGVLVLMRPF DYPLNAQARF SQNLLDQGSH PTSEKLLSVL RPASGHVADA
     LGITEGENVI HLRTLRRVNG VALCLIDHYF ADLTLWPTLQ RFDSGSLHDF LREQTGIALR
     RSQTRISARR AQAKECQRLE IPNMSPLLCV RTLNHRDGES SPAEYSVSLT RADMIEFTME
     H