PHNHY_PENOX
ID PHNHY_PENOX Reviewed; 72 AA.
AC P86807;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 1.
DT 25-MAY-2022, entry version 21.
DE RecName: Full=Phosphonoacetate hydrolase {ECO:0000303|PubMed:16129582};
DE Short=PA-hydrolase {ECO:0000303|PubMed:16129582};
DE EC=3.11.1.2;
DE Flags: Fragments;
OS Penicillium oxalicum.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=69781;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ABSENCE OF COFACTOR REQUIREMENT,
RP ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX PubMed=16129582; DOI=10.1016/j.resmic.2005.06.002;
RA Klimek-Ochab M., Raucci G., Lejczak B., Forlani G.;
RT "Phosphonoacetate hydrolase from Penicillium oxalicum: purification and
RT properties, phosphate starvation-independent expression, and partial
RT sequencing.";
RL Res. Microbiol. 157:125-135(2006).
RN [2] {ECO:0000305}
RP CATALYTIC ACTIVITY, ABSENCE OF COFACTOR REQUIREMENT, ACTIVITY REGULATION,
RP AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=12770709; DOI=10.1016/s0378-1097(03)00301-x;
RA Klimek-Ochab M., Lejczak B., Forlani G.;
RT "A metal-independent hydrolase from a Penicillium oxalicum strain able to
RT use phosphonoacetic acid as the only phosphorus source.";
RL FEMS Microbiol. Lett. 222:205-209(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + phosphonoacetate = acetate + H(+) + phosphate;
CC Xref=Rhea:RHEA:16749, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30089, ChEBI:CHEBI:43474, ChEBI:CHEBI:57488; EC=3.11.1.2;
CC Evidence={ECO:0000269|PubMed:12770709, ECO:0000269|PubMed:16129582};
CC -!- COFACTOR:
CC Note=Unlike bacterial phosphonoacetate hydrolase, does not require zinc
CC as a cofactor. {ECO:0000269|PubMed:12770709,
CC ECO:0000269|PubMed:16129582};
CC -!- ACTIVITY REGULATION: Unaffected by EDTA or Ca(2+), Co(2+), Cu(2+),
CC Mg(2+), Mn(2+), Ni(2+) and Zn(2+). {ECO:0000269|PubMed:12770709,
CC ECO:0000269|PubMed:16129582}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.8 mM for phosphonoacetate {ECO:0000269|PubMed:12770709,
CC ECO:0000269|PubMed:16129582};
CC pH dependence:
CC Optimum pH is 8.3. High activity between pH 7.5 and 8.6, rapidly
CC loses activity below pH 7.0. Loss of activity below pH 6.3 is
CC irreversible. {ECO:0000269|PubMed:12770709,
CC ECO:0000269|PubMed:16129582};
CC Temperature dependence:
CC Optimum temperature is 52 degrees Celsius.
CC {ECO:0000269|PubMed:12770709, ECO:0000269|PubMed:16129582};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:16129582}.
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DR AlphaFoldDB; P86807; -.
DR GO; GO:0047400; F:phosphonoacetate hydrolase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.720.10; -; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Hydrolase.
FT CHAIN <1..>72
FT /note="Phosphonoacetate hydrolase"
FT /id="PRO_0000402577"
FT UNSURE 4
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 5
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 8
FT /note="M or F"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 10
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 20
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 27
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 36
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 47
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 50
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 55
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 61
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 66
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 68
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 69
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT UNSURE 70
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:16129582"
FT NON_CONS 14..15
FT /evidence="ECO:0000303|PubMed:16129582"
FT NON_CONS 32..33
FT /evidence="ECO:0000303|PubMed:16129582"
FT NON_CONS 41..42
FT /evidence="ECO:0000303|PubMed:16129582"
FT NON_TER 1
FT /evidence="ECO:0000303|PubMed:16129582"
FT NON_TER 72
FT /evidence="ECO:0000303|PubMed:16129582"
SQ SEQUENCE 72 AA; 7812 MW; B4726EF8BEAEFB00 CRC64;
GSTLLEQMSL HGVRHAPGSL EANDFFLAND QRHGALGSFV RADEHDLSTL GDHPLRSHGG
LSEQQLPLLL SR
