ID   PHNI_RHIME              Reviewed;         368 AA.
AC   Q52986;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI;
DE            Short=RPnTP synthase subunit PhnI;
DE            EC=2.7.8.37;
DE   AltName: Full=Ribose 1-methylphosphonate 5-triphosphate synthase nucleosidase subunit;
GN   Name=phnI; OrderedLocusNames=RB1453; ORFNames=SMb20761;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RA   McLean P.A., Liu C.M., Sookdeo C.C., Cannon F.C.;
RT   "Characterization of a gene cluster involved in utilization of glyphosate
RT   and other phosphonates in Rhizobium meliloti.";
RL   Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Together with PhnG, PhnH and PhnL is required for the
CC       transfer of the ribose triphosphate moiety from ATP to methyl
CC       phosphonate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + methylphosphonate = adenine + alpha-D-ribose 1-
CC         methylphosphonate 5-triphosphate; Xref=Rhea:RHEA:34679,
CC         ChEBI:CHEBI:16708, ChEBI:CHEBI:30616, ChEBI:CHEBI:68684,
CC         ChEBI:CHEBI:68823; EC=2.7.8.37;
CC   -!- SIMILARITY: Belongs to the PhnI family. {ECO:0000305}.
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DR   EMBL; M96263; AAA26352.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49853.1; -; Genomic_DNA.
DR   PIR; E96023; E96023.
DR   RefSeq; NP_437993.1; NC_003078.1.
DR   RefSeq; WP_003530629.1; NC_003078.1.
DR   AlphaFoldDB; Q52986; -.
DR   SMR; Q52986; -.
DR   STRING; 266834.SM_b20761; -.
DR   EnsemblBacteria; CAC49853; CAC49853; SM_b20761.
DR   GeneID; 61601362; -.
DR   KEGG; sme:SM_b20761; -.
DR   PATRIC; fig|266834.11.peg.6379; -.
DR   eggNOG; COG3626; Bacteria.
DR   HOGENOM; CLU_063686_0_0_5; -.
DR   OMA; AQFTRGY; -.
DR   BioCyc; MetaCyc:MON-19920; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0061693; F:alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019634; P:organic phosphonate metabolic process; IEA:InterPro.
DR   InterPro; IPR008773; PhnI.
DR   Pfam; PF05861; PhnI; 1.
DR   PIRSF; PIRSF007313; PhnI; 1.
PE   3: Inferred from homology;
KW   Plasmid; Reference proteome; Transferase.
FT   CHAIN           1..368
FT                   /note="Alpha-D-ribose 1-methylphosphonate 5-triphosphate
FT                   synthase subunit PhnI"
FT                   /id="PRO_0000058396"
SQ   SEQUENCE   368 AA;  40775 MW;  DF317422EC02BCE0 CRC64;
     MYVAVKGGEA AIANAHRLLA DRRRGDRALP AITIDQVVEQ LGLAVDRVMA EASLYDRALA
     ALAVRQARGD MIEAIFILRA YRTTLPRFGY CEPVDTAKMK VERRVSATYK DLPGGQLLGP
     TFDYTHRLLD PSLIGEEPVD EPVLKEPGEH VMRVSDILDG EGLIEGDGKM PEGHVAGDLT
     REPMEFPMAR DLRLQALARG DEGFLLALAY STQRGYGRTH PFVGEVRIGE VEVELELPEL
     GFAVSLGVIR VTECQMVNQF KGSSRQPPQF TRGYGLVFGQ SERKAMSMSL VDRALRTDEF
     DEDIVAPAQD QEFVISHADN VQATGFVEHL KLPHYVDFQA ELDLVRRMRR EHDAAQAGGK
     DGMKEAAE