ID   PHNJ_RHIME              Reviewed;         297 AA.
AC   Q52987;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase;
DE            Short=PRPn C-P lyase;
DE            EC=4.7.1.1 {ECO:0000250|UniProtKB:P16688};
GN   Name=phnJ; OrderedLocusNames=RB1454; ORFNames=SMb20762;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RA   McLean P.A., Liu C.M., Sookdeo C.C., Cannon F.C.;
RT   "Characterization of a gene cluster involved in utilization of glyphosate
RT   and other phosphonates in Rhizobium meliloti.";
RL   Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Catalyzes the breakage of the C-P bond in alpha-D-ribose 1-
CC       methylphosphonate 5-phosphate (PRPn) forming alpha-D-ribose 1,2-cyclic
CC       phosphate 5-phosphate (PRcP). {ECO:0000250|UniProtKB:P16688}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + alpha-D-ribose 1-methylphosphonate 5-phosphate + S-
CC         adenosyl-L-methionine = 5'-deoxyadenosine + A + alpha-D-ribose 1,2-
CC         cyclic phosphate 5-phosphate + H(+) + L-methionine + methane;
CC         Xref=Rhea:RHEA:34707, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16183, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:68686,
CC         ChEBI:CHEBI:68687; EC=4.7.1.1;
CC         Evidence={ECO:0000250|UniProtKB:P16688};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000250|UniProtKB:P16688};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit.
CC       {ECO:0000250|UniProtKB:P16688};
CC   -!- SIMILARITY: Belongs to the PhnJ family. {ECO:0000305}.
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DR   EMBL; M96263; AAA26353.1; -; Genomic_DNA.
DR   EMBL; AL591985; CAC49854.1; -; Genomic_DNA.
DR   PIR; F96023; F96023.
DR   RefSeq; NP_437994.1; NC_003078.1.
DR   RefSeq; WP_003530631.1; NC_003078.1.
DR   AlphaFoldDB; Q52987; -.
DR   SMR; Q52987; -.
DR   STRING; 266834.SM_b20762; -.
DR   EnsemblBacteria; CAC49854; CAC49854; SM_b20762.
DR   GeneID; 61601363; -.
DR   KEGG; sme:SM_b20762; -.
DR   PATRIC; fig|266834.11.peg.6380; -.
DR   eggNOG; COG3627; Bacteria.
DR   HOGENOM; CLU_063386_0_0_5; -.
DR   OMA; MVYQVPI; -.
DR   BioCyc; MetaCyc:MON-19922; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0098848; F:alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019700; P:organic phosphonate catabolic process; IEA:InterPro.
DR   InterPro; IPR010306; PhnJ.
DR   Pfam; PF06007; PhnJ; 1.
DR   PIRSF; PIRSF011468; PhnJ; 1.
DR   SFLD; SFLDF00379; Phosphonate_metabolism_(PhnJ); 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Lyase; Metal-binding; Plasmid;
KW   Reference proteome; S-adenosyl-L-methionine.
FT   CHAIN           1..297
FT                   /note="Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-
FT                   lyase"
FT                   /id="PRO_0000058398"
SQ   SEQUENCE   297 AA;  33383 MW;  F115CBC20302A54C CRC64;
     MNDLATYNFA YLDEQTKRMI RRAILKAIAI PGYQVPFASR EMPMPYGWGT GGVQVTASIL
     GPDDVLKVID QGADDTTNAV SIRAFFQKVA DVAVTTRTTE ATIIQTRHRI PEEQLREGQT
     LVYQVPIPEP LRFLEPRETE TRKMHALEEY GLMHVKLYED IARNGHIATT YAYPVKVEGR
     YVMDPSPTPK FDNPKMHMSE ALQLFGAGRE KRIYAVPPYT EVVSLDFEDH PFEVQKFDKP
     CALCGAENVY LDEVVLDDKG GRMFVCSDTD HCEDRRAHGH AGAMLAPAAK ESQEAAE