ID   PHNS_DESMU              Reviewed;          32 AA.
AC   P13062;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Periplasmic [NiFe] hydrogenase small subunit;
DE            EC=1.12.2.1;
DE   AltName: Full=NiFe hydrogenlyase small chain;
DE   Flags: Fragment;
GN   Name=hydA;
OS   Desulfovibrio multispirans.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfovibrionales;
OC   Desulfovibrionaceae; Desulfovibrio.
OX   NCBI_TaxID=32018;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=3322275; DOI=10.1016/0006-291x(87)90376-7;
RA   Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E.,
RA   Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M.,
RA   Moura I., Moura J.J.G., Patil D., Huynh B.H.;
RT   "Identification of three classes of hydrogenase in the genus,
RT   Desulfovibrio.";
RL   Biochem. Biophys. Res. Commun. 149:369-377(1987).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 Fe(III)-[cytochrome c3] + H2 = 2 Fe(II)-[cytochrome c3] + 2
CC         H(+); Xref=Rhea:RHEA:20625, Rhea:RHEA-COMP:11576, Rhea:RHEA-
CC         COMP:11577, ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:29033,
CC         ChEBI:CHEBI:29034; EC=1.12.2.1;
CC   -!- COFACTOR:
CC       Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000250};
CC       Note=Binds 1 [3Fe-4S] cluster. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 2 [4Fe-4S] clusters. {ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Periplasm.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit
CC       family. {ECO:0000305}.
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DR   PIR; E27480; E27480.
DR   AlphaFoldDB; P13062; -.
DR   SMR; P13062; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0047806; F:cytochrome-c3 hydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.700; -; 1.
DR   InterPro; IPR037024; NiFe_Hase_small_N_sf.
PE   1: Evidence at protein level;
KW   3Fe-4S; 4Fe-4S; Direct protein sequencing; Iron; Iron-sulfur;
KW   Metal-binding; Oxidoreductase; Periplasm.
FT   CHAIN           1..>32
FT                   /note="Periplasmic [NiFe] hydrogenase small subunit"
FT                   /id="PRO_0000204354"
FT   BINDING         17
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         20
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   NON_TER         32
SQ   SEQUENCE   32 AA;  3504 MW;  A6D20619B0B011EB CRC64;
     ALTGRRPSVV YLHAAQCTGC SEALLRTTKP FR