PHO12_ARATH
ID PHO12_ARATH Reviewed; 807 AA.
AC Q6R8G8; Q0WV82; Q8S8M8;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Phosphate transporter PHO1 homolog 2;
DE AltName: Full=Protein PHO1 homolog 2;
DE Short=AtPHO1;H2;
GN Name=PHO1-H2; OrderedLocusNames=At2g03260; ORFNames=T18E12.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=15122012; DOI=10.1104/pp.103.037945;
RA Wang Y., Ribot C., Rezzonico E., Poirier Y.;
RT "Structure and expression profile of the Arabidopsis PHO1 gene family
RT indicates a broad role in inorganic phosphate homeostasis.";
RL Plant Physiol. 135:400-411(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 207-807.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May transport inorganic phosphate (Pi). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots and leaves.
CC {ECO:0000269|PubMed:15122012}.
CC -!- INDUCTION: Not induced by Pi deficiency. {ECO:0000269|PubMed:15122012}.
CC -!- SIMILARITY: Belongs to the SYG1 (TC 2.A.94) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM15032.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY507954; AAR99484.1; -; mRNA.
DR EMBL; AC005313; AAM15032.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05680.1; -; Genomic_DNA.
DR EMBL; AK226889; BAE98966.1; -; mRNA.
DR PIR; T02700; T02700.
DR RefSeq; NP_178425.2; NM_126377.2.
DR AlphaFoldDB; Q6R8G8; -.
DR STRING; 3702.AT2G03260.1; -.
DR iPTMnet; Q6R8G8; -.
DR PaxDb; Q6R8G8; -.
DR PRIDE; Q6R8G8; -.
DR EnsemblPlants; AT2G03260.1; AT2G03260.1; AT2G03260.
DR GeneID; 814855; -.
DR Gramene; AT2G03260.1; AT2G03260.1; AT2G03260.
DR KEGG; ath:AT2G03260; -.
DR Araport; AT2G03260; -.
DR TAIR; locus:2056911; AT2G03260.
DR eggNOG; KOG1162; Eukaryota.
DR HOGENOM; CLU_006116_2_0_1; -.
DR InParanoid; Q6R8G8; -.
DR OrthoDB; 536327at2759; -.
DR PhylomeDB; Q6R8G8; -.
DR PRO; PR:Q6R8G8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6R8G8; baseline and differential.
DR Genevisible; Q6R8G8; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0000822; F:inositol hexakisphosphate binding; IBA:GO_Central.
DR GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; IBA:GO_Central.
DR CDD; cd14476; SPX_PHO1_like; 1.
DR InterPro; IPR004342; EXS_C.
DR InterPro; IPR034092; PHO1_SPX.
DR InterPro; IPR004331; SPX_dom.
DR Pfam; PF03124; EXS; 1.
DR Pfam; PF03105; SPX; 1.
DR PROSITE; PS51380; EXS; 1.
DR PROSITE; PS51382; SPX; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Phosphate transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..807
FT /note="Phosphate transporter PHO1 homolog 2"
FT /id="PRO_0000398156"
FT TOPO_DOM 1..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..437
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 459..485
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..522
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..543
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 544..678
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 679..699
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 700..723
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 724..744
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 745..807
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 1..348
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT DOMAIN 607..807
FT /note="EXS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00712"
FT REGION 219..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..236
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 535
FT /note="V -> A (in Ref. 1; AAR99484)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 807 AA; 93333 MW; FCD79C212232B4DA CRC64;
MKFGKELSSQ MVQEWQQAYV NYDYLKTLLK EIIKLKEKTN PPPPPHHAVP GEGISRKMTL
YRAFSGLVQT PGKKRQSSRQ SNYSSEIDIE EGKAPILVSK STHGLETTFL MTAEEGGEYE
LVFFRRLDDE FNRVEKFYKE KVEEVMKDAI MLNKQMDALI AFRVKVENPV GWGWEERTVE
MTRLASDIAT STAAIAASTP ARTRTMNPRA QAHMEAIQEG SFSRENEDED HGSVRGATGD
VKTSSLNTMR GARPAPIEVL DHIKINNTKA TPRSTIKGVL NSSSQNEIIF NRQNLNEVEE
KLKFAFVEFY QKLRLLKSYS FLNVLAFSKI LKKYDKITSR NASKSYMKMV DNSYLGSSDE
LMKLIQRVES TFIKHFANGH RRKGMNILRP QMKREKHRVT FSTGFSAGCI FSLIVALVAI
IRTRKTMPEA EHNTYMNTMF PLYSLFGFIV LHITMYAIDI YYWKRYRVNY AFIFGCKQGT
ELGYRQVLFL GFTIGTFALL CVLGNLDMEV NPKTKNFKPL TELLPLFLLV ALFVVLIMPF
HFLYRSTRFF FLTCLLHCLA APLYKVTLPD FFLGDQLTSQ VQALRSINFY ICYYGWGDFK
KRQNTCEASE IYIYSLYIVA SLPYLSRLLQ CMRRMIEERS LDQGYNGVKY LLTVIAVSLR
TAYGYEVKNT KNPTSHLKVL AGSSSILAAV FCTYWDFVHD WGLLNKTSKN RWLRDKLLIP
QKKVYFIAMI LNVVLRFAWL QTILNFEFEF LHKQTTLAVV ASLEIMRRGM WNFFRVENEH
LNNVGKFRAF KSVPLPFNYD EDDEKDD
