PHO15_ARATH
ID PHO15_ARATH Reviewed; 823 AA.
AC Q6R8G5; O81050;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Phosphate transporter PHO1 homolog 5;
DE AltName: Full=Protein PHO1 homolog 5;
DE Short=AtPHO1;H5;
GN Name=PHO1-H5; OrderedLocusNames=At2g03240; ORFNames=T18E12.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=15122012; DOI=10.1104/pp.103.037945;
RA Wang Y., Ribot C., Rezzonico E., Poirier Y.;
RT "Structure and expression profile of the Arabidopsis PHO1 gene family
RT indicates a broad role in inorganic phosphate homeostasis.";
RL Plant Physiol. 135:400-411(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May transport inorganic phosphate (Pi). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips, vascular cylinders of roots
CC and filaments, leaf petioles, stem, receptacle, stigma apex and anther
CC connective tissue. {ECO:0000269|PubMed:15122012}.
CC -!- INDUCTION: Not induced by Pi deficiency. {ECO:0000269|PubMed:15122012}.
CC -!- SIMILARITY: Belongs to the SYG1 (TC 2.A.94) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC34478.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY507957; AAR99487.1; -; mRNA.
DR EMBL; AC005313; AAC34478.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05678.1; -; Genomic_DNA.
DR PIR; T02702; T02702.
DR RefSeq; NP_178423.5; NM_126375.6.
DR AlphaFoldDB; Q6R8G5; -.
DR STRING; 3702.AT2G03240.1; -.
DR iPTMnet; Q6R8G5; -.
DR PaxDb; Q6R8G5; -.
DR PRIDE; Q6R8G5; -.
DR ProteomicsDB; 235080; -.
DR EnsemblPlants; AT2G03240.1; AT2G03240.1; AT2G03240.
DR GeneID; 814853; -.
DR Gramene; AT2G03240.1; AT2G03240.1; AT2G03240.
DR KEGG; ath:AT2G03240; -.
DR Araport; AT2G03240; -.
DR TAIR; locus:2056941; AT2G03240.
DR eggNOG; KOG1162; Eukaryota.
DR HOGENOM; CLU_006116_2_0_1; -.
DR InParanoid; Q6R8G5; -.
DR OMA; YFAMIWD; -.
DR OrthoDB; 536327at2759; -.
DR PhylomeDB; Q6R8G5; -.
DR PRO; PR:Q6R8G5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6R8G5; baseline and differential.
DR Genevisible; Q6R8G5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0000822; F:inositol hexakisphosphate binding; IBA:GO_Central.
DR GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; IBA:GO_Central.
DR CDD; cd14476; SPX_PHO1_like; 1.
DR InterPro; IPR004342; EXS_C.
DR InterPro; IPR034092; PHO1_SPX.
DR InterPro; IPR004331; SPX_dom.
DR Pfam; PF03124; EXS; 1.
DR Pfam; PF03105; SPX; 1.
DR PROSITE; PS51380; EXS; 1.
DR PROSITE; PS51382; SPX; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Phosphate transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..823
FT /note="Phosphate transporter PHO1 homolog 5"
FT /id="PRO_0000398159"
FT TOPO_DOM 1..420
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..459
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 481..505
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 527..542
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 543..563
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 564..694
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 695..715
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 716..739
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 740..760
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 761..823
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 1..368
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT DOMAIN 627..823
FT /note="EXS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00712"
FT REGION 69..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 474
FT /note="L -> P (in Ref. 1; AAR99487)"
FT /evidence="ECO:0000305"
FT CONFLICT 777
FT /note="A -> V (in Ref. 2; AAC34478)"
FT /evidence="ECO:0000305"
FT CONFLICT 790
FT /note="F -> I (in Ref. 1; AAR99487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 823 AA; 95053 MW; 068544056D1BCB58 CRC64;
MKFGKEFSSQ MVPEWHEAYM DYDYLKSQLK EIIKFKRKTN PHGPHHHHHH LLHRKMTLHR
AFSGLISTSP KKKKHHGHGG GHGGGHQIGH FSDSDDDIEE GIKHVTAPIL INSASHGYET
TFLMASEEGG EYETVFFRRL DDEFNKVEKF YKEKVEEVMK EAVMLEKQMD ALIAFRVKVE
HPDGWPWEER TVEMTQLASD VANSAAAVAA STPAGARSMK VGAQAHMEAI QEGGSSKAGK
SSDEEDDDDA EKEEDNGVSG EVSGDVRKMK AGRPPPIEVL DRVKFNHTKE TPRSTIKSVL
QASNLTELKF SRENLRKVEA KLRRAFVEFY QKLRLLKSYS FLNELAFSKI LKKYDKITSR
HASKSYMKMI DNSYLGSSDE VTRLVERVEA TFIKHFSNAN RSKGMNILRP KAKRERHRIT
FSTGFLGGCL FSLVVALFAI IRTRNILQEE GQKQYMNTMF PLYSLFGFVV LHILMYAGNI
YYWRRYRVNY SFIFGFKHGT ELGYRQVLFV GLSIGVFALL CILANLDMEV DPETKDYQAL
TELLPLFLLT GMFVVLVLPF NIFYRSSRFF FLTCLFHCLA APLYKVTLPD FLVGDQLTSQ
VQALRSIQFY ICHYGWGDYK HRINTCTESD AYNAFLFIVA VIPYVSRLLQ CLRRLFEEKN
PEQGYNGLKY FLTIVAVCLR TTYSVDEDNQ FIWRILAGIF SAIAAIFCTY WDLVYDWGLL
NRTSKNPWLR DKLLVPQKKV YFIAMILNIL LRFAWLQTVL DFNFSFMHRQ TMVAVVASLE
IIRRGIWNFF RLENEHLNNV GKYRAFKTVP LPFNYDEDDD KGN
