PHO18_ARATH
ID PHO18_ARATH Reviewed; 751 AA.
AC Q6R8G2; Q9C7H9;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Phosphate transporter PHO1 homolog 8;
DE AltName: Full=Protein PHO1 homolog 8;
DE Short=AtPHO1;H8;
GN Name=PHO1-H8; OrderedLocusNames=At1g35350; ORFNames=T9I1.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=15122012; DOI=10.1104/pp.103.037945;
RA Wang Y., Ribot C., Rezzonico E., Poirier Y.;
RT "Structure and expression profile of the Arabidopsis PHO1 gene family
RT indicates a broad role in inorganic phosphate homeostasis.";
RL Plant Physiol. 135:400-411(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May transport inorganic phosphate (Pi). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in root epidermis, leaf hydathodes,
CC trichomes and petioles, stem vascular cylinder, receptacle, stigma apex
CC and pollen grains. {ECO:0000269|PubMed:15122012}.
CC -!- INDUCTION: Not induced by Pi deficiency. {ECO:0000269|PubMed:15122012}.
CC -!- SIMILARITY: Belongs to the SYG1 (TC 2.A.94) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51461.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY507960; AAR99490.1; -; mRNA.
DR EMBL; AC069160; AAG51461.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31785.1; -; Genomic_DNA.
DR PIR; F86474; F86474.
DR RefSeq; NP_174768.2; NM_103232.3.
DR AlphaFoldDB; Q6R8G2; -.
DR SMR; Q6R8G2; -.
DR BioGRID; 25656; 1.
DR STRING; 3702.AT1G35350.1; -.
DR iPTMnet; Q6R8G2; -.
DR PaxDb; Q6R8G2; -.
DR PRIDE; Q6R8G2; -.
DR ProteomicsDB; 236157; -.
DR EnsemblPlants; AT1G35350.1; AT1G35350.1; AT1G35350.
DR GeneID; 840424; -.
DR Gramene; AT1G35350.1; AT1G35350.1; AT1G35350.
DR KEGG; ath:AT1G35350; -.
DR Araport; AT1G35350; -.
DR TAIR; locus:2206742; AT1G35350.
DR eggNOG; KOG1162; Eukaryota.
DR HOGENOM; CLU_006116_2_0_1; -.
DR InParanoid; Q6R8G2; -.
DR OMA; IMVSEMI; -.
DR OrthoDB; 536327at2759; -.
DR PhylomeDB; Q6R8G2; -.
DR PRO; PR:Q6R8G2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6R8G2; baseline and differential.
DR Genevisible; Q6R8G2; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0000822; F:inositol hexakisphosphate binding; IBA:GO_Central.
DR GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; IBA:GO_Central.
DR CDD; cd14476; SPX_PHO1_like; 1.
DR InterPro; IPR004342; EXS_C.
DR InterPro; IPR034092; PHO1_SPX.
DR InterPro; IPR004331; SPX_dom.
DR Pfam; PF03124; EXS; 1.
DR Pfam; PF03105; SPX; 1.
DR PROSITE; PS51380; EXS; 1.
DR PROSITE; PS51382; SPX; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Phosphate transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..751
FT /note="Phosphate transporter PHO1 homolog 8"
FT /id="PRO_0000398162"
FT TOPO_DOM 1..351
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..392
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..434
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 456..473
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 474..494
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 495..623
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 624..644
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 645..667
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 668..688
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 689..751
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 1..299
FT /note="SPX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714"
FT DOMAIN 558..751
FT /note="EXS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00712"
SQ SEQUENCE 751 AA; 87396 MW; F48A179E66B5951E CRC64;
MKFGKEYVAQ MIPEWQQAYM DYTCLKTILR EIKTSQKRSE SQGVLKRKLS GRRNFSGLTK
RYSRTFSSRD LENHDIMVHA TTGDDGFEKY ETTILKVSEV GRESELVFFK TLDLEFDKVN
RFYRSNVEEL VKEAVVLNRQ MDALIAYRIK LDQPSTSWSC SETVSVDINA LDSKEQKGKT
LAEEMGIKVE ENVSNGGDST KETAPEALSV LDRIRLNKNQ ENPLSTIRNV LKLSNKEDIK
FTKENLKKIE ERLKNVFIEF YRKLRHLKNY SFLNTLAISK IMKKYDKIAL RNAAKLYMEM
VDKSYLTSSD EINKLMLRVE SIFVEHFAGS NRSKGMNLLR PKVTKEKHRI TFSTGFFVGC
TVSLVIALGL FIHARNIMGA VGHKLYMETM FPLYSLFAFV VLHMIMYASN IYFWKRYRVN
YPFIFGFKEG TELGYGHVLL LSFGLGTLAL CAVLVNMDME MDPNTNDYKT ITELVPLFVV
ALVIAISVCP FNIFYRSSRF FFLMVLFRCI AAPLYKVNLP DFFLADQLTS QVQALRSLEF
YICYYGWGDF KQRQSTCKSS DVYSTFYFIV AVIPYWSRFL QCVRRLIEEK DVSQGFNALK
YLLTIVAVCL RTAFSINRGN DWKIAAWVFS GLATFYGTYW DIVYDWGLLH RPSKSWLREK
LLVPHKSVYY VAMVVNVVLR LAWLQTVLDF NISFLHRETM VALIAILEII RRGIWNFFRL
ENEHLNNVGK FRAFKSVPLP FNYDEEEDRD S
