PHO2_YEAST
ID PHO2_YEAST Reviewed; 559 AA.
AC P07269; D6VRP4;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 03-AUG-2022, entry version 195.
DE RecName: Full=Regulatory protein PHO2;
DE AltName: Full=General regulatory factor 10;
GN Name=PHO2; Synonyms=BAS2, GRF10; OrderedLocusNames=YDL106C; ORFNames=D2350;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3029672; DOI=10.1093/nar/15.1.233;
RA Sengstag C., Hinnen A.;
RT "The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a
RT regulatory protein with unusual aminoacid composition.";
RL Nucleic Acids Res. 15:233-246(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Berben G.;
RL Submitted (JAN-1989) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8896274;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1077::aid-yea8>3.0.co;2-z;
RA Saiz J.E., Buitrago M.J., Garcia R., Revuelta J.L., del Rey F.;
RT "The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces
RT cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1
RT genes, and six new open reading frames.";
RL Yeast 12:1077-1084(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP STRUCTURE, EXPRESSION, AND FUNCTION.
RX PubMed=3049251; DOI=10.1016/0378-1119(88)90367-8;
RA Berben G., Legrain M., Hilger F.;
RT "Studies on the structure, expression and function of the yeast regulatory
RT gene PHO2.";
RL Gene 66:307-312(1988).
RN [7]
RP DOMAIN HOMEOBOX.
RX PubMed=2896548; DOI=10.1016/0092-8674(88)90153-5;
RA Buerglin T.R.;
RT "The yeast regulatory gene PHO2 encodes a homeo box.";
RL Cell 53:339-340(1988).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-542, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Regulator in phosphate metabolism and acts as a derepressor
CC of another central regulator PHO5. Binds to the upstream activator
CC sequence (UAS) of PHO5. It also binds to the TRP4, HIS4, and CYC1
CC promoters. {ECO:0000269|PubMed:3049251}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 6420 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X05062; CAA28729.1; -; Genomic_DNA.
DR EMBL; M22259; AAA34866.1; -; Genomic_DNA.
DR EMBL; X95644; CAA64906.1; -; Genomic_DNA.
DR EMBL; Z74154; CAA98673.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11754.1; -; Genomic_DNA.
DR PIR; A25872; A25872.
DR RefSeq; NP_010177.1; NM_001180165.1.
DR AlphaFoldDB; P07269; -.
DR SMR; P07269; -.
DR BioGRID; 31956; 182.
DR DIP; DIP-13N; -.
DR IntAct; P07269; 18.
DR STRING; 4932.YDL106C; -.
DR iPTMnet; P07269; -.
DR MaxQB; P07269; -.
DR PaxDb; P07269; -.
DR PRIDE; P07269; -.
DR EnsemblFungi; YDL106C_mRNA; YDL106C; YDL106C.
DR GeneID; 851452; -.
DR KEGG; sce:YDL106C; -.
DR SGD; S000002264; PHO2.
DR VEuPathDB; FungiDB:YDL106C; -.
DR eggNOG; KOG0486; Eukaryota.
DR HOGENOM; CLU_028430_2_0_1; -.
DR InParanoid; P07269; -.
DR OMA; NTHFATD; -.
DR BioCyc; YEAST:G3O-29507-MON; -.
DR PRO; PR:P07269; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P07269; protein.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:SGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; HDA:SGD.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0006338; P:chromatin remodeling; IMP:SGD.
DR GO; GO:0043388; P:positive regulation of DNA binding; IDA:SGD.
DR GO; GO:0045937; P:positive regulation of phosphate metabolic process; IGI:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:2000679; P:positive regulation of transcription regulatory region DNA binding; IMP:SGD.
DR GO; GO:0120213; P:regulation of histidine biosynthetic process; IMP:SGD.
DR GO; GO:0006141; P:regulation of purine nucleobase metabolic process; IDA:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..559
FT /note="Regulatory protein PHO2"
FT /id="PRO_0000049247"
FT DNA_BIND 77..136
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 16..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 534..559
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..158
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 534..548
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 542
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 559 AA; 63391 MW; BE20E396D6AA0281 CRC64;
MMEEFSYDHD FNTHFATDLD YLQHDQQQQQ QQQHDQQHNQ QQQPQPQPIQ TQNLEHDHDQ
HTNDMSASSN ASDSGPQRPK RTRAKGEALD VLKRKFEINP TPSLVERKKI SDLIGMPEKN
VRIWFQNRRA KLRKKQHGSN KDTIPSSQSR DIANDYDRGS TDNNLVTTTS TSSIFHDEDL
TFFDRIPLNS NNNYYFFDIC SITVGSWNRM KSGALQRRNF QSIKELRNLS PIKINNIMSN
ATDLMVLISK KNSEINYFFS AMANNTKILF RIFFPLSSVT NCSLTLETDD DIINSNNTSD
KNNSNTNNDD DNDDNSNEDN DNSSEDKRNA KDNFGELKLT VTRSPTFAVY FLNNAPDEDP
NLNNQWSICD DFSEGRQVND AFVGGSNIPH TLKGLQKSLR FMNSLILDYK SSNEILPTIN
TAIPTAAVPQ QNIAPPFLNT NSSATDSNPN TNLEDSLFFD HDLLSSSITN TNNGQGSNNG
RQASKDDTLN LLDTTVNSNN NHNANNEENH LAQEHLSNDA DIVANPNDHL LSLPTDSELP
NTPDFLKNTN ELTDEHRWI
