PHOB_PSEAE
ID PHOB_PSEAE Reviewed; 229 AA.
AC P23620;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Phosphate regulon transcriptional regulatory protein PhoB;
GN Name=phoB; OrderedLocusNames=PA5360;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2115874; DOI=10.1128/jb.172.8.4685-4689.1990;
RA Anba J., Bidaud M., Vasil M.L., Lazdunski A.;
RT "Nucleotide sequence of the Pseudomonas aeruginosa phoB gene, the
RT regulatory gene for the phosphate regulon.";
RL J. Bacteriol. 172:4685-4689(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=24023943; DOI=10.1371/journal.pone.0074444;
RA Blus-Kadosh I., Zilka A., Yerushalmi G., Banin E.;
RT "The effect of pstS and phoB on quorum sensing and swarming motility in
RT Pseudomonas aeruginosa.";
RL PLoS ONE 8:E74444-E74444(2013).
CC -!- FUNCTION: This protein is a positive regulator for the phosphate
CC regulon. Transcription of this operon is positively regulated by PhoB
CC and PhoR when phosphate is limited.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DISRUPTION PHENOTYPE: Loss of swarming in the presence and absence of
CC phosphate; double phoB-pstS deletions act like phoB deletions.
CC {ECO:0000269|PubMed:24023943}.
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DR EMBL; AE004091; AAG08745.1; -; Genomic_DNA.
DR PIR; A37775; A37775.
DR PIR; C82975; C82975.
DR RefSeq; NP_254047.1; NC_002516.2.
DR RefSeq; WP_003096653.1; NZ_QZGE01000020.1.
DR AlphaFoldDB; P23620; -.
DR SMR; P23620; -.
DR STRING; 287.DR97_2731; -.
DR PaxDb; P23620; -.
DR PRIDE; P23620; -.
DR DNASU; 878438; -.
DR EnsemblBacteria; AAG08745; AAG08745; PA5360.
DR GeneID; 878438; -.
DR KEGG; pae:PA5360; -.
DR PATRIC; fig|208964.12.peg.5617; -.
DR PseudoCAP; PA5360; -.
DR HOGENOM; CLU_000445_30_4_6; -.
DR InParanoid; P23620; -.
DR OMA; IDRVWGS; -.
DR PhylomeDB; P23620; -.
DR BioCyc; PAER208964:G1FZ6-5482-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR CollecTF; EXPREG_00000940; -.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0071978; P:bacterial-type flagellum-dependent swarming motility; IMP:PseudoCAP.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR GO; GO:0080040; P:positive regulation of cellular response to phosphate starvation; IMP:PseudoCAP.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEP:CollecTF.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR011879; Sig_transdc_resp-reg_PhoB.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR TIGRFAMs; TIGR02154; PhoB; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Phosphate transport; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Transport;
KW Two-component regulatory system.
FT CHAIN 1..229
FT /note="Phosphate regulon transcriptional regulatory protein
FT PhoB"
FT /id="PRO_0000081190"
FT DOMAIN 5..121
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 130..227
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 54
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 23
FT /note="L -> V (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="D -> H (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="V -> L (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 98
FT /note="V -> A (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="D -> H (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 162
FT /note="R -> G (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 180..181
FT /note="LL -> RR (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
FT CONFLICT 198..199
FT /note="VH -> MD (in Ref. 1; no nucleotide entry)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 229 AA; 25712 MW; AD7FAEFA7F8004A3 CRC64;
MVGKTILIVD DEAPIREMIA VALEMAGYEC LEAENTQQAH AVIVDRKPDL ILLDWMLPGT
SGIELARRLK RDELTVDIPI IMLTAKGEED NKIQGLEVGA DDYITKPFSP RELVARLKAV
LRRTGPGDSE APIEVGGLLL DPISHRVTID GKPAEMGPTE YRLLQFFMTH QERAYTRGQL
LDQVWGGNVY VEERTVDVHI RRLRKALGEV YENLVQTVRG TGYRFSTKS
