PHOB_SHIFL
ID PHOB_SHIFL Reviewed; 229 AA.
AC P45607;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 25-MAY-2022, entry version 152.
DE RecName: Full=Phosphate regulon transcriptional regulatory protein PhoB;
GN Name=phoB; OrderedLocusNames=SF0336, S0344;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M25-8 / Serotype 2a;
RX PubMed=7746146; DOI=10.1111/j.1365-2958.1995.tb02239.x;
RA Scholten M., Janssen R., Bogaarts C., van Strien J., Tommassen J.;
RT "The pho regulon of Shigella flexneri.";
RL Mol. Microbiol. 15:247-254(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: This protein is a positive regulator for the phosphate
CC regulon. Transcription of this operon is positively regulated by PhoB
CC and PhoR when phosphate is limited.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- PTM: Phosphorylated by PhoR. {ECO:0000250}.
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DR EMBL; X81000; CAA56927.1; -; Genomic_DNA.
DR EMBL; AE005674; AAN41994.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP15872.1; -; Genomic_DNA.
DR PIR; S61298; S61298.
DR RefSeq; NP_706287.1; NC_004337.2.
DR RefSeq; WP_000113936.1; NZ_QWST01000097.1.
DR RefSeq; WP_000113938.1; NZ_WPGW01000023.1.
DR AlphaFoldDB; P45607; -.
DR BMRB; P45607; -.
DR SMR; P45607; -.
DR STRING; 198214.SF0336; -.
DR EnsemblBacteria; AAN41994; AAN41994; SF0336.
DR EnsemblBacteria; AAP15872; AAP15872; S0344.
DR GeneID; 1027627; -.
DR KEGG; sfl:SF0336; -.
DR KEGG; sft:NCTC1_00346; -.
DR KEGG; sfx:S0344; -.
DR PATRIC; fig|198214.7.peg.384; -.
DR HOGENOM; CLU_000445_30_4_6; -.
DR OMA; IDRVWGS; -.
DR OrthoDB; 817710at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR011879; Sig_transdc_resp-reg_PhoB.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR TIGRFAMs; TIGR02154; PhoB; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Phosphate transport; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Transport;
KW Two-component regulatory system.
FT CHAIN 1..229
FT /note="Phosphate regulon transcriptional regulatory protein
FT PhoB"
FT /id="PRO_0000081193"
FT DOMAIN 4..120
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 129..227
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 53
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 140
FT /note="N -> D (in Ref. 1; CAA56927)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="R -> L (in Ref. 1; CAA56927)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 229 AA; 26431 MW; E3E63880059D8CA9 CRC64;
MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI LLDWMLPGGS
GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD DYITKPFSPK ELVARIKAVM
RRISPMAVEE VIKMQGLSLN PTSHRVMAGE EPLEMGPTEF KLLHFFMTHP ERVYSREQLL
NHVWGTNVYV EDRTVDVHIR RLRKALEPGG HDRMVQTVRG TGYRFSTRF
