PHOP_BACSU
ID PHOP_BACSU Reviewed; 240 AA.
AC P13792; O34804;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 4.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Alkaline phosphatase synthesis transcriptional regulatory protein PhoP;
GN Name=phoP; OrderedLocusNames=BSU29110;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=1454550; DOI=10.1093/nar/20.21.5848;
RA Lee J.W., Hulett F.M.;
RT "Nucleotide sequence of the phoP gene encoding PhoP, the response regulator
RT of the phosphate regulon of Bacillus subtilis.";
RL Nucleic Acids Res. 20:5848-5848(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3036763; DOI=10.1128/jb.169.7.2913-2916.1987;
RA Seki T., Yoshikawa H., Takahashi H., Saito H.;
RT "Cloning and nucleotide sequence of phoP, the regulatory gene for alkaline
RT phosphatase and phosphodiesterase in Bacillus subtilis.";
RL J. Bacteriol. 169:2913-2916(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP SEQUENCE REVISION TO 124.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
RC STRAIN=168 / SMY;
RX PubMed=8045898; DOI=10.1128/jb.176.15.4669-4679.1994;
RA Jin S., Sonenshein A.L.;
RT "Identification of two distinct Bacillus subtilis citrate synthase genes.";
RL J. Bacteriol. 176:4669-4679(1994).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 212-240.
RX PubMed=3142862; DOI=10.1128/jb.170.12.5935-5938.1988;
RA Seki T., Yoshikawa H., Takahashi H., Saito H.;
RT "Nucleotide sequence of the Bacillus subtilis phoR gene.";
RL J. Bacteriol. 170:5935-5938(1988).
CC -!- FUNCTION: Member of the two-component regulatory system PhoP/PhoR
CC involved in the regulation of alkaline phosphatase genes phoA and phoB
CC and of phosphodiesterase.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by PhoR. {ECO:0000305}.
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DR EMBL; X67676; CAA47908.1; -; Genomic_DNA.
DR EMBL; M16775; AAA22661.1; -; Genomic_DNA.
DR EMBL; AF008220; AAC00348.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14871.2; -; Genomic_DNA.
DR EMBL; U05257; AAA96344.1; -; Genomic_DNA.
DR EMBL; M23549; AAA22662.1; -; Genomic_DNA.
DR PIR; F69676; RGBSAP.
DR RefSeq; NP_390789.2; NC_000964.3.
DR RefSeq; WP_003229442.1; NZ_JNCM01000036.1.
DR PDB; 1MVO; X-ray; 1.60 A; A=1-135.
DR PDBsum; 1MVO; -.
DR AlphaFoldDB; P13792; -.
DR SMR; P13792; -.
DR STRING; 224308.BSU29110; -.
DR jPOST; P13792; -.
DR PaxDb; P13792; -.
DR PRIDE; P13792; -.
DR EnsemblBacteria; CAB14871; CAB14871; BSU_29110.
DR GeneID; 936644; -.
DR KEGG; bsu:BSU29110; -.
DR PATRIC; fig|224308.179.peg.3160; -.
DR eggNOG; COG0745; Bacteria.
DR InParanoid; P13792; -.
DR OMA; SYPRRVW; -.
DR PhylomeDB; P13792; -.
DR BioCyc; BSUB:BSU29110-MON; -.
DR EvolutionaryTrace; P13792; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cytoplasm; DNA-binding; Phosphate transport;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Transport; Two-component regulatory system.
FT CHAIN 1..240
FT /note="Alkaline phosphatase synthesis transcriptional
FT regulatory protein PhoP"
FT /id="PRO_0000081194"
FT DOMAIN 4..118
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 136..235
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 53
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 124
FT /note="A -> R (in Ref. 2; AAA22661 and 3; AAC00348)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="Y -> I (in Ref. 2; AAA22661)"
FT /evidence="ECO:0000305"
FT CONFLICT 211
FT /note="D -> PT (in Ref. 2; AAA22661)"
FT /evidence="ECO:0000305"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:1MVO"
FT HELIX 12..24
FT /evidence="ECO:0007829|PDB:1MVO"
FT STRAND 28..34
FT /evidence="ECO:0007829|PDB:1MVO"
FT HELIX 35..45
FT /evidence="ECO:0007829|PDB:1MVO"
FT STRAND 48..55
FT /evidence="ECO:0007829|PDB:1MVO"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:1MVO"
FT HELIX 61..70
FT /evidence="ECO:0007829|PDB:1MVO"
FT STRAND 77..81
FT /evidence="ECO:0007829|PDB:1MVO"
FT HELIX 89..94
FT /evidence="ECO:0007829|PDB:1MVO"
FT STRAND 99..104
FT /evidence="ECO:0007829|PDB:1MVO"
FT HELIX 107..119
FT /evidence="ECO:0007829|PDB:1MVO"
SQ SEQUENCE 240 AA; 27598 MW; E61A6FADBE513D97 CRC64;
MNKKILVVDD EESIVTLLQY NLERSGYDVI TASDGEEALK KAETEKPDLI VLDVMLPKLD
GIEVCKQLRQ QKLMFPILML TAKDEEFDKV LGLELGADDY MTKPFSPREV NARVKAILRR
SEIAAPSSEM KNDEMEGQIV IGDLKILPDH YEAYFKESQL ELTPKEFELL LYLGRHKGRV
LTRDLLLSAV WNYDFAGDTR IVDVHISHLR DKIENNTKKP IYIKTIRGLG YKLEEPKMNE
