PHOP_PSEAE
ID PHOP_PSEAE Reviewed; 225 AA.
AC Q9I4F9;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Two-component response regulator PhoP {ECO:0000303|PubMed:10564474};
GN Name=phoP; OrderedLocusNames=PA1179;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10564474; DOI=10.1046/j.1365-2958.1999.01600.x;
RA Macfarlane E.L., Kwasnicka A., Ochs M.M., Hancock R.E.;
RT "PhoP-PhoQ homologues in Pseudomonas aeruginosa regulate expression of the
RT outer-membrane protein OprH and polymyxin B resistance.";
RL Mol. Microbiol. 34:305-316(1999).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11021929; DOI=10.1099/00221287-146-10-2543;
RA Macfarlane E.L.A., Kwasnicka A., Hancock R.E.W.;
RT "Role of Pseudomonas aeruginosa PhoP-phoQ in resistance to antimicrobial
RT cationic peptides and aminoglycosides.";
RL Microbiology 146:2543-2554(2000).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=16707691; DOI=10.1128/jb.00053-06;
RA McPhee J.B., Bains M., Winsor G., Lewenza S., Kwasnicka A., Brazas M.D.,
RA Brinkman F.S., Hancock R.E.;
RT "Contribution of the PhoP-PhoQ and PmrA-PmrB two-component regulatory
RT systems to Mg2+-induced gene regulation in Pseudomonas aeruginosa.";
RL J. Bacteriol. 188:3995-4006(2006).
RN [5]
RP FUNCTION.
RX PubMed=24994873; DOI=10.1093/jac/dku238;
RA Lee J.Y., Chung E.S., Na I.Y., Kim H., Shin D., Ko K.S.;
RT "Development of colistin resistance in pmrA-, phoP-, parR- and cprR-
RT inactivated mutants of Pseudomonas aeruginosa.";
RL J. Antimicrob. Chemother. 69:2966-2971(2014).
CC -!- FUNCTION: Member of the two-component regulatory system PhoP/PhoQ that
CC plays a role in the regulation of resistance towards polymyxin B and
CC cationic antimicrobial peptides in response to limiting concentrations
CC of Mg(2+) (PubMed:10564474, PubMed:11021929, PubMed:24994873).
CC Functions as a transcriptional activator by direct binding to a cis-
CC acting sequence upstream of the target gene promoters including oprH
CC and pmrH promoters (PubMed:16707691). {ECO:0000269|PubMed:10564474,
CC ECO:0000269|PubMed:11021929, ECO:0000269|PubMed:16707691,
CC ECO:0000269|PubMed:24994873}.
CC -!- DISRUPTION PHENOTYPE: Deletion leads to loss of expression of the
CC outer-membrane protein OprH which is part of the response towards
CC Mg(2+) starvation. {ECO:0000269|PubMed:10564474,
CC ECO:0000269|PubMed:11021929}.
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DR EMBL; AE004091; AAG04568.1; -; Genomic_DNA.
DR PIR; H83497; H83497.
DR RefSeq; NP_249870.1; NC_002516.2.
DR RefSeq; WP_003082436.1; NZ_QZGE01000006.1.
DR AlphaFoldDB; Q9I4F9; -.
DR SMR; Q9I4F9; -.
DR STRING; 287.DR97_755; -.
DR PaxDb; Q9I4F9; -.
DR PRIDE; Q9I4F9; -.
DR DNASU; 879194; -.
DR EnsemblBacteria; AAG04568; AAG04568; PA1179.
DR GeneID; 879194; -.
DR KEGG; pae:PA1179; -.
DR PATRIC; fig|208964.12.peg.1224; -.
DR PseudoCAP; PA1179; -.
DR HOGENOM; CLU_000445_30_1_6; -.
DR InParanoid; Q9I4F9; -.
DR OMA; HHQQVVP; -.
DR PhylomeDB; Q9I4F9; -.
DR BioCyc; PAER208964:G1FZ6-1204-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0071073; P:positive regulation of phospholipid biosynthetic process; IMP:PseudoCAP.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..225
FT /note="Two-component response regulator PhoP"
FT /id="PRO_0000449417"
FT DOMAIN 2..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 124..222
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 51
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 225 AA; 25650 MW; F02E64EB1BBCF24B CRC64;
MKLLVVEDEA LLRHHLYTRL GEQGHVVDAV PDAEEALYRV SEYHHDLAVI DLGLPGMSGL
DLIRELRSQG KSFPILILTA RGNWQDKVEG LAAGADDYVV KPFQFEELEA RLNALLRRSS
GFVQSTIEAG PLVLDLNRKQ ALVEEQPVAL TAYEYRILEY LMRHHQQVVA KERLMEQLYP
DDEERDANVI EVLVGRLRRK LEACGGFKPI DTVRGQGYLF TERCR
