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PHOP_SALT1
ID   PHOP_SALT1              Reviewed;         224 AA.
AC   D0ZV90;
DT   13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Virulence transcriptional regulatory protein PhoP;
GN   Name=phoP; OrderedLocusNames=STM14_1409;
OS   Salmonella typhimurium (strain 14028s / SGSC 2262).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=588858;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=19897643; DOI=10.1128/jb.01233-09;
RA   Jarvik T., Smillie C., Groisman E.A., Ochman H.;
RT   "Short-term signatures of evolutionary change in the Salmonella enterica
RT   serovar typhimurium 14028 genome.";
RL   J. Bacteriol. 192:560-567(2010).
RN   [2]
RP   FUNCTION.
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=8392513; DOI=10.1128/jb.175.14.4475-4484.1993;
RA   Behlau I., Miller S.I.;
RT   "A PhoP-repressed gene promotes Salmonella typhimurium invasion of
RT   epithelial cells.";
RL   J. Bacteriol. 175:4475-4484(1993).
RN   [3]
RP   AUTOREGULATION.
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=7543474; DOI=10.1128/jb.177.15.4364-4371.1995;
RA   Soncini F.C., Garcia Vescovi E., Groisman E.A.;
RT   "Transcriptional autoregulation of the Salmonella typhimurium phoPQ
RT   operon.";
RL   J. Bacteriol. 177:4364-4371(1995).
RN   [4]
RP   REGULATION BY MG(2+).
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=8548821; DOI=10.1016/s0092-8674(00)81003-x;
RA   Garcia Vescovi E., Soncini F.C., Groisman E.A.;
RT   "Mg2+ as an extracellular signal: environmental regulation of Salmonella
RT   virulence.";
RL   Cell 84:165-174(1996).
RN   [5]
RP   FUNCTION.
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=10084994; DOI=10.1128/iai.67.4.1614-1622.1999;
RA   van Velkinburgh J.C., Gunn J.S.;
RT   "PhoP-PhoQ-regulated loci are required for enhanced bile resistance in
RT   Salmonella spp.";
RL   Infect. Immun. 67:1614-1622(1999).
RN   [6]
RP   FUNCTION.
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=10775270; DOI=10.1093/emboj/19.8.1861;
RA   Kox L.F.F., Woesten M.M.S.M., Groisman E.A.;
RT   "A small protein that mediates the activation of a two-component system by
RT   another two-component system.";
RL   EMBO J. 19:1861-1872(2000).
RN   [7]
RP   FUNCTION, AND INDUCTION BY CATIONIC ANTIMICROBIAL PEPTIDES (CAMP).
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=14507376; DOI=10.1046/j.1365-2958.2003.03675.x;
RA   Bader M.W., Navarre W.W., Shiau W., Nikaido H., Frye J.G., McClelland M.,
RA   Fang F.C., Miller S.I.;
RT   "Regulation of Salmonella typhimurium virulence gene expression by cationic
RT   antimicrobial peptides.";
RL   Mol. Microbiol. 50:219-230(2003).
RN   [8]
RP   INDUCTION, AND PHOSPHORYLATION BY PHOQ.
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=16096064; DOI=10.1016/j.cell.2005.05.030;
RA   Bader M.W., Sanowar S., Daley M.E., Schneider A.R., Cho U., Xu W.,
RA   Klevit R.E., Le Moual H., Miller S.I.;
RT   "Recognition of antimicrobial peptides by a bacterial sensor kinase.";
RL   Cell 122:461-472(2005).
RN   [9]
RP   FUNCTION.
RC   STRAIN=14028s / SGSC 2262;
RX   PubMed=15948951; DOI=10.1111/j.1365-2958.2005.04668.x;
RA   Bijlsma J.J.E., Groisman E.A.;
RT   "The PhoP/PhoQ system controls the intramacrophage type three secretion
RT   system of Salmonella enterica.";
RL   Mol. Microbiol. 57:85-96(2005).
CC   -!- FUNCTION: Member of the two-component regulatory system PhoP/PhoQ which
CC       regulates the expression of genes involved in virulence, adaptation to
CC       acidic and low Mg(2+) environments and resistance to host defense
CC       antimicrobial peptides. Essential for intramacrophage survival of
CC       S.typhimurium. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP,
CC       resulting in the expression of PhoP-activated genes (PAG) and
CC       repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+),
CC       PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG
CC       and may lead to expression of some PRG. Essential for transcription of
CC       spiC inside macrophages by controlling the expression of the two-
CC       component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional
CC       and post-transcriptional levels respectively. Promotes expression of
CC       the two-component regulatory system PmrA/PmrB via activation of pmrD
CC       gene. Is required to attenuate bacterial growth within fibroblast cells
CC       and to enhance bacterial resistance to bile in intestinal cells.
CC       Negatively regulates prgH, which is required for invasion of epithelial
CC       cells. PhoP uses multiple mechanisms to promote transcription and
CC       activates promoters for PAG at low (uM range) Mg(2+) concentrations.
CC       Probably involved in acid tolerance. {ECO:0000269|PubMed:10084994,
CC       ECO:0000269|PubMed:10775270, ECO:0000269|PubMed:14507376,
CC       ECO:0000269|PubMed:15948951, ECO:0000269|PubMed:8392513}.
CC   -!- SUBUNIT: Monomer in the inactive, unphosphorylated state and dimer in
CC       the active, phosphorylated state. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: The phoP/phoQ operon is positively autoregulated by both
CC       PhoP and PhoQ in a Mg(2+)-dependent manner. Repressed by RcsB via sigma
CC       factor RpoS. Induced by antimicrobial peptides (similar to those in
CC       macrophages) and low Mg(2+) concentrations.
CC       {ECO:0000269|PubMed:14507376, ECO:0000269|PubMed:16096064}.
CC   -!- PTM: Phosphorylated by PhoQ. {ECO:0000269|PubMed:16096064}.
CC   -!- MISCELLANEOUS: PhoP/PhoQ-signaling cascade, which activated virulence
CC       membrane proteins (PagC, PagO, PagD, PagK, PgtE and PhoN), is induced
CC       by cationic antimicrobial peptides (CAMP) (polymyxin, alpha-helical
CC       peptide C18G and sheet peptide protegrin-1) at sublethal
CC       concentrations.
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DR   EMBL; CP001363; ACY87895.1; -; Genomic_DNA.
DR   RefSeq; WP_000986522.1; NZ_CP043402.1.
DR   AlphaFoldDB; D0ZV90; -.
DR   SMR; D0ZV90; -.
DR   IntAct; D0ZV90; 1.
DR   EnsemblBacteria; ACY87895; ACY87895; STM14_1409.
DR   KEGG; seo:STM14_1409; -.
DR   PATRIC; fig|588858.6.peg.1379; -.
DR   HOGENOM; CLU_000445_30_1_6; -.
DR   OMA; QLWGYPP; -.
DR   BioCyc; SENT588858:STM14_RS06665-MON; -.
DR   PHI-base; PHI:6110; -.
DR   Proteomes; UP000002695; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Growth regulation; Phosphoprotein;
KW   Repressor; Transcription; Transcription regulation;
KW   Two-component regulatory system; Virulence.
FT   CHAIN           1..224
FT                   /note="Virulence transcriptional regulatory protein PhoP"
FT                   /id="PRO_0000424530"
FT   DOMAIN          3..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        125..223
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         52
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   224 AA;  25633 MW;  3A90B2FDC328C7B2 CRC64;
     MMRVLVVEDN ALLRHHLKVQ LQDSGHQVDA AEDAREADYY LNEHLPDIAI VDLGLPDEDG
     LSLIRRWRSS DVSLPVLVLT AREGWQDKVE VLSSGADDYV TKPFHIEEVM ARMQALMRRN
     SGLASQVINI PPFQVDLSRR ELSVNEEVIK LTAFEYTIME TLIRNNGKVV SKDSLMLQLY
     PDAELRESHT IDVLMGRLRK KIQAQYPHDV ITTVRGQGYL FELR
 
 
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