PHOR_HAEIN
ID PHOR_HAEIN Reviewed; 425 AA.
AC P71380;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Phosphate regulon sensor protein PhoR;
DE EC=2.7.13.3;
GN Name=phoR; OrderedLocusNames=HI_1378;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Member of the two-component regulatory system PhoR/PhoB
CC involved in the phosphate regulon genes expression. PhoR may function
CC as a membrane-associated protein kinase that phosphorylates PhoB in
CC response to environmental signals (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
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DR EMBL; L42023; AAC23024.1; -; Genomic_DNA.
DR RefSeq; NP_439530.1; NC_000907.1.
DR RefSeq; WP_010869197.1; NC_000907.1.
DR STRING; 71421.HI_1378; -.
DR EnsemblBacteria; AAC23024; AAC23024; HI_1378.
DR KEGG; hin:HI_1378; -.
DR PATRIC; fig|71421.8.peg.1433; -.
DR eggNOG; COG5002; Bacteria.
DR HOGENOM; CLU_000445_89_2_6; -.
DR OMA; GIGAWNE; -.
DR PhylomeDB; P71380; -.
DR BioCyc; HINF71421:G1GJ1-1404-MON; -.
DR BRENDA; 2.7.13.3; 2529.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004721; F:phosphoprotein phosphatase activity; IBA:GO_Central.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IBA:GO_Central.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR CDD; cd00082; HisKA; 1.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR014310; Sig_transdc_His_kinase_PhoR.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00091; PAS; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR TIGRFAMs; TIGR02966; phoR_proteo; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Kinase; Membrane;
KW Nucleotide-binding; Phosphate transport; Phosphoprotein;
KW Reference proteome; Transferase; Transmembrane; Transmembrane helix;
KW Transport; Two-component regulatory system.
FT CHAIN 1..425
FT /note="Phosphate regulon sensor protein PhoR"
FT /id="PRO_0000074847"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 89..156
FT /note="PAS"
FT DOMAIN 201..415
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 204
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ SEQUENCE 425 AA; 49859 MW; 47220F1DD7F32941 CRC64;
MKKILNFIVE INLAIIISLF TSDFILWFAI ILLLILAWHH INEYRLLKYL NLKQDNKFSL
LQLGTFSQTE AYHRHQIYKE KCASLRLLSQ INKNIKYLPD AIIICQHNGN ISWCNSIAPQ
MFDFCWDKKV QENIFDVIFY EQFKHYFFSP KKRRPLVLLT YNQRYIEVQS HAYNSHMILV
IARDITDMIH LLNSRQKFLS NINHELRTPL TVLQGYLEIL ADNNIQNPLQ KKAIXAMQEQ
SQRMEHLLQQ FNFLAKIETT SDKDFRKFDM SAMINSLRKD TDILNTYNHH IEFIIQPNII
IFGNESQLRS AVSNLIYNAI KHSGKQCHIQ IQWETCEQGI KFNVIDNGVG ISPQHIPHLT
ERFYRVDESR SHLTGGSGLG LAIVKHTLLQ YHSHLNIEST ETKGSSFSFI IPKRFVISKN
NKEIQ
