PHOSP_BDV1
ID PHOSP_BDV1 Reviewed; 201 AA.
AC P0C798; P26668;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Phosphoprotein;
DE Short=P protein;
DE AltName: Full=p23;
DE AltName: Full=p24;
GN Name=P/X;
OS Borna disease virus 1 (BoDV-1).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Bornaviridae; Orthobornavirus.
OX NCBI_TaxID=1714621;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
OH NCBI_TaxID=9352; Bradypodidae (three-fingered sloths).
OH NCBI_TaxID=9925; Capra hircus (Goat).
OH NCBI_TaxID=9850; Cervidae (deer).
OH NCBI_TaxID=109474; Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew).
OH NCBI_TaxID=9788; Equidae (horses).
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=56798; Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis).
OH NCBI_TaxID=9844; Lama glama (Llama).
OH NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
OH NCBI_TaxID=9940; Ovis aries (Sheep).
OH NCBI_TaxID=8801; Struthio camelus (Common ostrich).
OH NCBI_TaxID=9455; Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus).
OH NCBI_TaxID=30538; Vicugna pacos (Alpaca) (Lama pacos).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=Giessen / HE/80-3;
RX PubMed=1538196; DOI=10.1099/0022-1317-73-2-413;
RA Thierer J., Riehle H., Grebenstein O., Binz T., Herzog S., Thiedemann N.,
RA Stitz L., Rott R., Lottspeich F., Niemann H.;
RT "The 24K protein of Borna disease virus.";
RL J. Gen. Virol. 73:413-416(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Clone B8;
RX PubMed=1895063; DOI=10.1099/0022-1317-72-9-2251;
RA Richt J.A., Vandewoude S., Zink M.C., Narayan O., Clements J.E.;
RT "Analysis of Borna disease virus-specific RNAs in infected cells and
RT tissues.";
RL J. Gen. Virol. 72:2251-2255(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=7906311; DOI=10.1128/jvi.68.3.1382-1396.1994;
RA Cubitt B., Oldstone C., de la Torre J.C.;
RT "Sequence and genome organization of Borna disease virus.";
RL J. Virol. 68:1382-1396(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Halle B1/91;
RX PubMed=8254777; DOI=10.1128/jvi.68.1.63-68.1994;
RA Schneider P.A., Briese T., Zimmermann W., Ludwig H., Lipkin W.I.;
RT "Sequence conservation in field and experimental isolates of Borna disease
RT virus.";
RL J. Virol. 68:63-68(1994).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Clone B8;
RX PubMed=8317098; DOI=10.1006/viro.1993.1364;
RA Pyper J.M., Richt J.A., Brown L., Rott R., Narayan O., Clements J.E.;
RT "Genomic organization of the structural proteins of borna disease virus
RT revealed by a cDNA clone encoding the 38-kDa protein.";
RL Virology 195:229-238(1993).
RN [6]
RP PHOSPHORYLATION.
RX PubMed=9268312; DOI=10.1074/jbc.272.35.21818;
RA Schwemmle M., De B., Shi L., Banerjee A., Lipkin W.I.;
RT "Borna disease virus P-protein is phosphorylated by protein kinase Cepsilon
RT and casein kinase II.";
RL J. Biol. Chem. 272:21818-21823(1997).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=9811710; DOI=10.1128/jvi.72.12.9755-9762.1998;
RA Shoya Y., Kobayashi T., Koda T., Ikuta K., Kakinuma M., Kishi M.;
RT "Two proline-rich nuclear localization signals in the amino- and carboxyl-
RT terminal regions of the Borna disease virus phosphoprotein.";
RL J. Virol. 72:9755-9762(1998).
RN [8]
RP INTERACTION WITH N AND X PROTEINS.
RX PubMed=9535888; DOI=10.1074/jbc.273.15.9007;
RA Schwemmle M., Salvatore M., Shi L., Richt J., Lee C.H., Lipkin W.I.;
RT "Interactions of the borna disease virus P, N, and X proteins and their
RT functional implications.";
RL J. Biol. Chem. 273:9007-9012(1998).
RN [9]
RP ALTERNATIVE INITIATION.
RX PubMed=11080477; DOI=10.1006/viro.2000.0592;
RA Kobayashi T., Watanabe M., Kamitani W., Tomonaga K., Ikuta K.;
RT "Translation initiation of a bicistronic mRNA of Borna disease virus: a 16-
RT kDa phosphoprotein is initiated at an internal start codon.";
RL Virology 277:296-305(2000).
RN [10]
RP REVIEW.
RX PubMed=11815287; DOI=10.2741/a789;
RA Ikuta K., Ibrahim M.S., Kobayashi T., Tomonaga K.;
RT "Borna disease virus and infection in humans.";
RL Front. Biosci. 7:470-495(2002).
RN [11]
RP INTERACTION WITH HUMAN TBK1.
RX PubMed=16155125; DOI=10.1073/pnas.0502883102;
RA Unterstab G., Ludwig S., Anton A., Planz O., Dauber B., Krappmann D.,
RA Heins G., Ehrhardt C., Wolff T.;
RT "Viral targeting of the interferon-beta-inducing Traf family member-
RT associated NF-kappa-B activator (TANK)-binding kinase-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:13640-13645(2005).
CC -!- FUNCTION: Essential component of the RNA polymerase transcription and
CC replication complex. Acts as a scaffold which brings L in close
CC proximity to the N-RNA complex. Plays a role in the segregation of the
CC viral genome in host daughter cells during mitosis by interacting with
CC host HMGB1, a host chromatin-remodeling DNA architectural protein,
CC thereby stabilizing RNP on chromosomes. Interacts with host TBK1 and
CC thus interferes with activation of cellular antiviral state. Inhibits
CC cellular histone acetyltransferase activities.
CC {ECO:0000250|UniProtKB:P0C799}.
CC -!- SUBUNIT: Homomultimer; only active in its oligomeric state. Interacts
CC with nucleoprotein/N. Interacts with matrix/M protein. Interacts with
CC host TBK1. Interacts with polymerase L. Interacts with host HMGB1; this
CC interaction is required to stabilize RNP on chromosomes.
CC {ECO:0000250|UniProtKB:P0C799}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000250|UniProtKB:P0C799}. Host
CC cytoplasm {ECO:0000250|UniProtKB:P0C799}. Note=P subcellular
CC localization is modulated by the interaction with protein X.
CC {ECO:0000250|UniProtKB:P0C799}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=p24;
CC IsoId=P0C798-1; Sequence=Displayed;
CC Name=p16;
CC IsoId=P0C798-2; Sequence=VSP_018912;
CC -!- PTM: Phosphorylated by host PKC epsilon and casein kinase II.
CC {ECO:0000269|PubMed:9268312}.
CC -!- MISCELLANEOUS: The P/X gene has two overlapping open reading frames.
CC One encodes the P protein and the other the X protein. The P (p24), X
CC and P'(p16) proteins are produced by ribosomal leaky scanning.
CC {ECO:0000269|PubMed:11080477}.
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DR EMBL; X60701; CAA43112.1; -; mRNA.
DR EMBL; D10473; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; L27077; AAA20664.1; -; Genomic_RNA.
DR EMBL; S67507; AAB29215.1; -; Genomic_RNA.
DR EMBL; S62821; AAB27263.1; -; Genomic_RNA.
DR PIR; C37475; C37475.
DR PIR; JQ1294; JQ1294.
DR PIR; JQ1295; JQ1295.
DR PIR; JQ1407; WMWVBD.
DR SMR; P0C798; -.
DR Proteomes; UP000185272; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
DR InterPro; IPR009517; BDV_P24.
DR Pfam; PF06595; BDV_P24; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Direct protein sequencing; Host cytoplasm;
KW Host nucleus; Host-virus interaction;
KW Inhibition of host innate immune response by virus;
KW Inhibition of host RLR pathway by virus; Phosphoprotein;
KW Viral immunoevasion.
FT CHAIN 1..201
FT /note="Phosphoprotein"
FT /id="PRO_0000045266"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 29..36
FT /note="Nuclear localization signal 1"
FT MOTIF 181..193
FT /note="Nuclear localization signal 2"
FT COMPBIAS 9..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..55
FT /note="Missing (in isoform p16)"
FT /evidence="ECO:0000305"
FT /id="VSP_018912"
FT VARIANT 4
FT /note="R -> G (in strain: C6)"
FT VARIANT 24
FT /note="E -> K (in strain: Giessen)"
SQ SEQUENCE 201 AA; 22463 MW; 4B3AE42D15ABB4D1 CRC64;
MATRPSSLVD SLEDEEDPQT LRRERSGSPR PRKIPRNALT QPVDQLLKDL RKNPSMISDP
DQRTGREQLS NDELIKKLVT ELAENSMIEA EEVRGTLGDI SARIEAGFES LSALQVETIQ
TAQRCDHSDS IRILGENIKI LDRSMKTMME TMKLMMEKVD LLYASTAVGT SAPMLPSHPA
PPRIYPQLPS APTADEWDII P
