PHOSP_NIPAV
ID PHOSP_NIPAV Reviewed; 709 AA.
AC Q9IK91; Q5K4E1; Q8QU02;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Phosphoprotein;
DE Short=Protein P;
GN Name=P/V/C;
OS Nipah virus.
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae;
OC Henipavirus.
OX NCBI_TaxID=121791;
OH NCBI_TaxID=58060; Cynopterus brachyotis (Lesser short-nosed fruit bat) (Pachysoma brachyotis).
OH NCBI_TaxID=58065; Eonycteris spelaea (Lesser dawn bat) (Macroglossus spelaeus).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9405; Pteropus hypomelanus (Island flying fox) (Variable flying fox).
OH NCBI_TaxID=132908; Pteropus vampyrus (Large flying fox).
OH NCBI_TaxID=153297; Scotophilus kuhlii (Lesser asiatic yellow bat).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=10827955; DOI=10.1126/science.288.5470.1432;
RA Chua K.B., Bellini W.J., Rota P.A., Harcourt B.H., Tamin A., Lam S.K.,
RA Ksiazek T.G., Rollin P.E., Zaki S.R., Shieh W., Goldsmith C.S.,
RA Gubler D.J., Roehrig J.T., Eaton B., Gould A.R., Olson J., Field H.,
RA Daniels P., Ling A.E., Peters C.J., Anderson L.J., Mahy B.W.;
RT "Nipah virus: a recently emergent deadly paramyxovirus.";
RL Science 288:1432-1435(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11504554; DOI=10.1006/viro.2001.1026;
RA Harcourt B.H., Tamin A., Halpin K., Ksiazek T.G., Rollin P.E.,
RA Bellini W.J., Rota P.A.;
RT "Molecular characterization of the polymerase gene and genomic termini of
RT Nipah virus.";
RL Virology 287:192-201(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate UMMC1, and Isolate UMMC2;
RX PubMed=11514724; DOI=10.1099/0022-1317-82-9-2151;
RA Chan Y.P., Chua K.B., Koh C.L., Lim M.E., Lam S.K.;
RT "Complete nucleotide sequences of Nipah virus isolates from Malaysia.";
RL J. Gen. Virol. 82:2151-2155(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Malaysian flying-fox;
RX PubMed=11880045; DOI=10.1016/s1286-4579(01)01522-2;
RA Chua K.B., Koh C.L., Hooi P.S., Wee K.F., Khong J.H., Chua B.H., Chan Y.P.,
RA Lim M.E., Lam S.K.;
RT "Isolation of Nipah virus from Malaysian island flying-foxes.";
RL Microbes Infect. 4:145-151(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate NV/MY/99/UM-0128, Isolate NV/MY/99/VRI-1413, and
RC Isolate NV/MY/99/VRI-2794;
RX PubMed=15663869; DOI=10.3201/eid1012.040452;
RA Abubakar S., Chang L.Y., Mohdali A.R., Sharifah S.H., Yusoff K., Zamrod Z.;
RT "Isolation and molecular identification of Nipah virus from pigs.";
RL Emerg. Infect. Dis. 10:2228-2230(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate NV/MY/99/VRI-0626;
RA Abubakar S., Li-Yen C., Mohdali A.R., Sharifah S.H.;
RT "Identification of a new Nipah virus strain from pigs.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH HOST STAT1.
RX PubMed=15140960; DOI=10.1128/jvi.78.11.5633-5641.2004;
RA Shaw M.L., Garcia-Sastre A., Palese P., Basler C.F.;
RT "Nipah virus V and W proteins have a common STAT1-binding domain yet
RT inhibit STAT1 activation from the cytoplasmic and nuclear compartments,
RT respectively.";
RL J. Virol. 78:5633-5641(2004).
CC -!- FUNCTION: Essential component of the RNA polymerase transcription and
CC replication complex. Binds the viral ribonucleocapsid and positions the
CC L polymerase on the template (By similarity). May play a role to
CC prevent the establishment of cellular antiviral state by binding to
CC host STAT1 in the cytoplasm. This activity is not as strong as that of
CC V and W. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with host STAT1. {ECO:0000269|PubMed:15140960}.
CC -!- INTERACTION:
CC Q9IK91; Q9IK92: N; NbExp=5; IntAct=EBI-16116432, EBI-16116412;
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm.
CC -!- RNA EDITING: Modified_positions=406; Note=Partially edited. RNA editing
CC at this position consists of an insertion of one or two guanine
CC nucleotides. The sequence displayed here is the P protein, derived from
CC the unedited RNA. The edited RNA gives rise to the V protein (+1G) (AC
CC Q997F2), and the W protein (+2G) (AC P0C1C7).;
CC -!- MISCELLANEOUS: The P/V/C gene has two overlapping open reading frames.
CC One encodes the P/V/W proteins and the other the C protein.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF212302; AAF73378.1; -; Genomic_RNA.
DR EMBL; AY029767; AAK50541.1; -; Genomic_RNA.
DR EMBL; AY029768; AAK50549.1; -; Genomic_RNA.
DR EMBL; AJ564621; CAD92348.1; -; Genomic_RNA.
DR EMBL; AJ564622; CAD92354.1; -; Genomic_RNA.
DR EMBL; AJ564623; CAD92360.1; -; Genomic_RNA.
DR EMBL; AF376747; AAM13402.1; -; Genomic_RNA.
DR EMBL; AJ627196; CAF25494.1; -; Genomic_RNA.
DR RefSeq; NP_112022.1; NC_002728.1.
DR PDB; 4CO6; X-ray; 2.50 A; D/E/F=1-50.
DR PDB; 4GJW; X-ray; 3.00 A; A/B/C/D/E/F/G/H=471-580.
DR PDB; 6EB8; X-ray; 2.50 A; A/B/C/D/E/F/G/H=470-578.
DR PDB; 6EB9; X-ray; 1.90 A; A=470-578.
DR PDBsum; 4CO6; -.
DR PDBsum; 4GJW; -.
DR PDBsum; 6EB8; -.
DR PDBsum; 6EB9; -.
DR SASBDB; Q9IK91; -.
DR SMR; Q9IK91; -.
DR DIP; DIP-61042N; -.
DR IntAct; Q9IK91; 9.
DR MINT; Q9IK91; -.
DR GeneID; 920952; -.
DR KEGG; vg:920952; -.
DR Proteomes; UP000002330; Genome.
DR Proteomes; UP000007527; Genome.
DR Proteomes; UP000008676; Genome.
DR Proteomes; UP000100567; Genome.
DR Proteomes; UP000110983; Genome.
DR Proteomes; UP000130871; Genome.
DR Proteomes; UP000170143; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0039689; P:negative stranded viral RNA replication; IDA:UniProtKB.
DR GO; GO:0039697; P:negative stranded viral RNA transcription; IDA:UniProtKB.
DR DisProt; DP00699; -.
DR InterPro; IPR004897; P/V_Pprotein_paramyxoviral.
DR InterPro; IPR028243; Paramyxo_P/V_N.
DR InterPro; IPR035430; Paramyxo_PNT.
DR InterPro; IPR025909; Soyouz_module.
DR Pfam; PF03210; Paramyx_P_V_C; 1.
DR Pfam; PF13825; Paramyxo_P_V_N; 1.
DR Pfam; PF14320; Paramyxo_PNT; 1.
DR Pfam; PF14313; Soyouz_module; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Host cytoplasm;
KW Interferon antiviral system evasion; Phosphoprotein; Reference proteome;
KW RNA editing; Viral RNA replication; Virion.
FT CHAIN 1..709
FT /note="Phosphoprotein"
FT /id="PRO_0000236013"
FT REGION 53..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 560..592
FT /note="L protein binding"
FT /evidence="ECO:0000250"
FT COILED 498..576
FT /evidence="ECO:0000250"
FT COMPBIAS 60..78
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 440..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 257
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT MOD_RES 350
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
FT VARIANT 206
FT /note="P -> L (in strain: Isolate Malaysian flying-fox)"
FT VARIANT 274
FT /note="S -> R (in strain: Isolate NV/MY/99/VRI-0626)"
FT VARIANT 304
FT /note="T -> A (in strain: Isolate NV/MY/99/VRI-0626)"
FT VARIANT 378
FT /note="E -> K (in strain: Isolate NV/MY/99/VRI-0626)"
FT HELIX 1..28
FT /evidence="ECO:0007829|PDB:4CO6"
FT HELIX 480..483
FT /evidence="ECO:0007829|PDB:6EB9"
FT TURN 484..487
FT /evidence="ECO:0007829|PDB:6EB9"
FT HELIX 490..503
FT /evidence="ECO:0007829|PDB:6EB9"
FT HELIX 508..541
FT /evidence="ECO:0007829|PDB:6EB9"
FT HELIX 545..577
FT /evidence="ECO:0007829|PDB:6EB9"
SQ SEQUENCE 709 AA; 78303 MW; 0A20AC5A9FD9319A CRC64;
MDKLELVNDG LNIIDFIQKN QKEIQKTYGR SSIQQPSIKD QTKAWEDFLQ CTSGESEQVE
GGMSKDDGDV ERRNLEDLSS TSPTDGTIGK RVSNTRDWAE GSDDIQLDPV VTDVVYHDHG
GECTGYGFTS SPERGWSDYT SGANNGNVCL VSDAKMLSYA PEIAVSKEDR ETDLVHLENK
LSTTGLNPTA VPFTLRNLSD PAKDSPVIAE HYYGLGVKEQ NVGPQTSRNV NLDSIKLYTS
DDEEADQLEF EDEFAGSSSE VIVGISPEDE EPSSVGGKPN ESIGRTIEGQ SIRDNLQAKD
NKSTDVPGAG PKDSAVKEEP PQKRLPMLAE EFECSGSEDP IIRELLKENS LINCQQGKDA
QPPYHWSIER SISPDKTEIV NGAVQTADRQ RPGTPMPKSR GIPIKKGTDA KYPSAGTENV
PGSKSGATRH VRGSPPYQEG KSVNAENVQL NASTAVKETD KSEVNPVDDN DSLDDKYIMP
SDDFSNTFFP HDTDRLNYHA DHLGDYDLET LCEESVLMGV INSIKLINLD MRLNHIEEQV
KEIPKIINKL ESIDRVLAKT NTALSTIEGH LVSMMIMIPG KGKGERKGKN NPELKPVIGR
DILEQQSLFS FDNVKNFRDG SLTNEPYGAA VQLREDLILP ELNFEETNAS QFVPMADDSS
RDVIKTLIRT HIKDRELRSE LIGYLNKAEN DEEIQEIANT VNDIIDGNI
