A53E_DROME
ID A53E_DROME Reviewed; 120 AA.
AC O46199; Q6GUV6; Q6VBF9; Q6VBG0; Q6VBG1; Q8MZD8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Accessory gland protein Acp53Ea;
DE Flags: Precursor;
GN Name=Acp53Ea; ORFNames=CG8622;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Male accessory gland;
RX PubMed=9474779; DOI=10.1016/s0965-1748(97)00056-8;
RA Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W.,
RA Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K.;
RT "New genes for male accessory gland proteins in Drosophila melanogaster.";
RL Insect Biochem. Mol. Biol. 27:825-834(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=WS1, WS12, WS16, WS19, WS26, WS47, WS49, WS56, WS6, WS9, ZIM2,
RC ZIM26, ZIM30, ZIM42, and ZIM56;
RX PubMed=11102381; DOI=10.1093/genetics/156.4.1879;
RA Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G.;
RT "Molecular population genetics of male accessory gland proteins in
RT Drosophila.";
RL Genetics 156:1879-1888(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NFS 5.1, NFS 5.2, NFS 5.3, NFS 5.4, NFS 6.1, NFS 6.2, NFS 6.3,
RC NFS 6.4, NFS 7.8, SFS 1.1, SFS 1.2, SFS 1.3, SFS 1.4, SFS 2.2, SFS 2.3,
RC SFS 2.4, SFS 3.1, SFS 3.2, SFS 3.3, and SFS 3.4;
RX PubMed=14761057; DOI=10.1554/03-335;
RA Panhuis T.M., Swanson W.J., Nunney L.;
RT "Population genetics of accessory gland proteins and sexual behavior in
RT Drosophila melanogaster populations from Evolution Canyon.";
RL Evolution 57:2785-2791(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M01, M06, M09, M16, M26, and M47;
RX PubMed=15215320; DOI=10.1093/molbev/msh195;
RA Holloway A.K., Begun D.J.;
RT "Molecular evolution and population genetics of duplicated accessory gland
RT protein genes in Drosophila.";
RL Mol. Biol. Evol. 21:1625-1628(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Responsible for physiological and behavioral changes in mated
CC female flies. {ECO:0000269|PubMed:9474779}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Main cells of accessory gland and seminal fluid.
CC {ECO:0000269|PubMed:9474779}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM29231.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
CC Sequence=AAM29231.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U85760; AAB96384.1; -; mRNA.
DR EMBL; AY010593; AAG37375.1; -; Genomic_DNA.
DR EMBL; AY010594; AAG37376.1; -; Genomic_DNA.
DR EMBL; AY010595; AAG37377.1; -; Genomic_DNA.
DR EMBL; AY010596; AAG37378.1; -; Genomic_DNA.
DR EMBL; AY010597; AAG37379.1; -; Genomic_DNA.
DR EMBL; AY010598; AAG37380.1; -; Genomic_DNA.
DR EMBL; AY010599; AAG37381.1; -; Genomic_DNA.
DR EMBL; AY010600; AAG37382.1; -; Genomic_DNA.
DR EMBL; AY010601; AAG37383.1; -; Genomic_DNA.
DR EMBL; AY010602; AAG37384.1; -; Genomic_DNA.
DR EMBL; AY010603; AAG37385.1; -; Genomic_DNA.
DR EMBL; AY010604; AAG37386.1; -; Genomic_DNA.
DR EMBL; AY010605; AAG37387.1; -; Genomic_DNA.
DR EMBL; AY010606; AAG37388.1; -; Genomic_DNA.
DR EMBL; AY010607; AAG37389.1; -; Genomic_DNA.
DR EMBL; AY344265; AAR04580.1; -; Genomic_DNA.
DR EMBL; AY344266; AAR04581.1; -; Genomic_DNA.
DR EMBL; AY344267; AAR04582.1; -; Genomic_DNA.
DR EMBL; AY344268; AAR04583.1; -; Genomic_DNA.
DR EMBL; AY344269; AAR04584.1; -; Genomic_DNA.
DR EMBL; AY344270; AAR04585.1; -; Genomic_DNA.
DR EMBL; AY344271; AAR04586.1; -; Genomic_DNA.
DR EMBL; AY344272; AAR04587.1; -; Genomic_DNA.
DR EMBL; AY344273; AAR04588.1; -; Genomic_DNA.
DR EMBL; AY344274; AAR04589.1; -; Genomic_DNA.
DR EMBL; AY344275; AAR04590.1; -; Genomic_DNA.
DR EMBL; AY344276; AAR04591.1; -; Genomic_DNA.
DR EMBL; AY344277; AAR04592.1; -; Genomic_DNA.
DR EMBL; AY344278; AAR04593.1; -; Genomic_DNA.
DR EMBL; AY344279; AAR04594.1; -; Genomic_DNA.
DR EMBL; AY344280; AAR04595.1; -; Genomic_DNA.
DR EMBL; AY344281; AAR04596.1; -; Genomic_DNA.
DR EMBL; AY344282; AAR04597.1; -; Genomic_DNA.
DR EMBL; AY344283; AAR04598.1; -; Genomic_DNA.
DR EMBL; AY344284; AAR04599.1; -; Genomic_DNA.
DR EMBL; AY635241; AAT49147.1; -; Genomic_DNA.
DR EMBL; AY635242; AAT49148.1; -; Genomic_DNA.
DR EMBL; AY635243; AAT49149.1; -; Genomic_DNA.
DR EMBL; AY635244; AAT49150.1; -; Genomic_DNA.
DR EMBL; AY635245; AAT49151.1; -; Genomic_DNA.
DR EMBL; AY635246; AAT49152.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF57959.1; -; Genomic_DNA.
DR EMBL; AY113226; AAM29231.1; ALT_FRAME; mRNA.
DR RefSeq; NP_477151.1; NM_057803.5.
DR AlphaFoldDB; O46199; -.
DR BioGRID; 62583; 2.
DR DIP; DIP-22625N; -.
DR IntAct; O46199; 3.
DR STRING; 7227.FBpp0086228; -.
DR PaxDb; O46199; -.
DR PRIDE; O46199; -.
DR EnsemblMetazoa; FBtr0087080; FBpp0086228; FBgn0015584.
DR GeneID; 36874; -.
DR KEGG; dme:Dmel_CG8622; -.
DR CTD; 36874; -.
DR FlyBase; FBgn0015584; Acp53Ea.
DR VEuPathDB; VectorBase:FBgn0015584; -.
DR GeneTree; ENSGT00940000176318; -.
DR HOGENOM; CLU_165703_0_0_1; -.
DR InParanoid; O46199; -.
DR OMA; WLACNRI; -.
DR OrthoDB; 1584218at2759; -.
DR PhylomeDB; O46199; -.
DR BioGRID-ORCS; 36874; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36874; -.
DR PRO; PR:O46199; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0015584; Expressed in male reproductive gland and 12 other tissues.
DR Genevisible; O46199; DM.
DR GO; GO:0005615; C:extracellular space; HDA:FlyBase.
DR GO; GO:0019953; P:sexual reproduction; HEP:FlyBase.
DR GO; GO:0046692; P:sperm competition; TAS:FlyBase.
DR InterPro; IPR009392; ACP53EA.
DR Pfam; PF06313; ACP53EA; 1.
PE 2: Evidence at transcript level;
KW Behavior; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..120
FT /note="Accessory gland protein Acp53Ea"
FT /id="PRO_0000020584"
FT VARIANT 7
FT /note="T -> A (in strain: ZIM56)"
FT VARIANT 14
FT /note="A -> D (in strain: M47)"
FT VARIANT 21
FT /note="T -> L (in strain: M47)"
FT VARIANT 22
FT /note="E -> K (in strain: ZIM26 and ZIM42)"
FT VARIANT 27
FT /note="I -> V (in strain: ZIM56)"
FT VARIANT 70
FT /note="N -> K (in strain: M09 and WS9)"
FT VARIANT 72
FT /note="Y -> C (in strain: SFS 3.1)"
FT VARIANT 83
FT /note="V -> D (in strain: ZIM30)"
FT VARIANT 108
FT /note="Y -> C (in strain: ZIM2)"
SQ SEQUENCE 120 AA; 13607 MW; C576B846B4DC2871 CRC64;
MKLIKVTLVF SLLALVFVAQ TEAQNPIWEN WLACNRIGTK ALASLLRETI PTVRNLLNCI
DFNPPTDIGN SYLSKLKLYY ELVKRGALDK TQCLIVPLKE SVRLLRPYVK SLETNKCLGE
