PHOT_BACSU
ID PHOT_BACSU Reviewed; 261 AA.
AC O34627;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Blue-light photoreceptor;
DE AltName: Full=Photoactive flavo-yellow protein;
DE AltName: Full=Phototropin homolog;
GN Name=pfyP; Synonyms=ytvA; OrderedLocusNames=BSU30340;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, COMPLEX SUGGESTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=11157946; DOI=10.1128/jb.183.4.1329-1338.2001;
RA Akbar S., Gaidenko T.A., Kang C.M., O'Reilly M., Devine K.M., Price C.W.;
RT "New family of regulators in the environmental signaling pathway which
RT activates the general stress transcription factor sigma(B) of Bacillus
RT subtilis.";
RL J. Bacteriol. 183:1329-1338(2001).
RN [4]
RP CHARACTERIZATION, AND 3D-STRUCTURE MODELING OF 25-126.
RX PubMed=11964249; DOI=10.1016/s0006-3495(02)75604-x;
RA Losi A., Polverini E., Quest B., Gaertner W.;
RT "First evidence for phototropin-related blue-light receptors in
RT prokaryotes.";
RL Biophys. J. 82:2627-2634(2002).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 20-147, SUBUNIT, AND FMN-BINDING
RP AT CYS-62.
RX PubMed=17764689; DOI=10.1016/j.jmb.2007.07.039;
RA Moglich A., Moffat K.;
RT "Structural basis for light-dependent signaling in the dimeric LOV domain
RT of the photosensor YtvA.";
RL J. Mol. Biol. 373:112-126(2007).
CC -!- FUNCTION: Exhibits the same spectroscopical features and blue-light
CC induced photochemistry as plants phototropins, with the reversible
CC formation of a blue-shifted photoproduct, assigned to an FMN-cysteine
CC thiol adduct. Although it is a positive regulator in the activation of
CC the environmental signaling branch of the general stress transcription
CC factor sigma-B, its precise role is undetermined.
CC {ECO:0000269|PubMed:11157946}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:17764689}.
CC -!- PTM: FMN binds covalently to cysteine after exposure to blue light and
CC this bond is spontaneously broken in the dark.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene have a twofold decreased
CC response to salt and ethanol stress, and a somewhat reduced response to
CC energy stress, indicating that PhoT is a positive regulator of sigma-B
CC activity. It acts independently of both RsbRA and RsbRB (YkoB).
CC {ECO:0000269|PubMed:11157946}.
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DR EMBL; AF008220; AAC00382.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15012.1; -; Genomic_DNA.
DR PIR; A70002; A70002.
DR RefSeq; NP_390912.1; NC_000964.3.
DR RefSeq; WP_004399022.1; NZ_JNCM01000036.1.
DR PDB; 2MWG; NMR; -; A/B=2-261.
DR PDB; 2PR5; X-ray; 1.45 A; A/B=20-147.
DR PDB; 2PR6; X-ray; 1.95 A; A/B=20-147.
DR PDB; 4GCZ; X-ray; 2.30 A; A/B=1-126.
DR PDBsum; 2MWG; -.
DR PDBsum; 2PR5; -.
DR PDBsum; 2PR6; -.
DR PDBsum; 4GCZ; -.
DR AlphaFoldDB; O34627; -.
DR BMRB; O34627; -.
DR SASBDB; O34627; -.
DR SMR; O34627; -.
DR STRING; 224308.BSU30340; -.
DR PaxDb; O34627; -.
DR DNASU; 937254; -.
DR EnsemblBacteria; CAB15012; CAB15012; BSU_30340.
DR GeneID; 937254; -.
DR KEGG; bsu:BSU30340; -.
DR PATRIC; fig|224308.179.peg.3291; -.
DR eggNOG; COG1366; Bacteria.
DR eggNOG; COG2202; Bacteria.
DR InParanoid; O34627; -.
DR OMA; FWNELNI; -.
DR PhylomeDB; O34627; -.
DR BioCyc; BSUB:BSU30340-MON; -.
DR EvolutionaryTrace; O34627; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR Pfam; PF13426; PAS_9; 1.
DR Pfam; PF01740; STAS; 1.
DR SMART; SM00086; PAC; 1.
DR SMART; SM00091; PAS; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR TIGRFAMs; TIGR00229; sensory_box; 1.
DR PROSITE; PS50113; PAC; 1.
DR PROSITE; PS50112; PAS; 1.
DR PROSITE; PS50801; STAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromophore; Flavoprotein; FMN; Photoreceptor protein;
KW Receptor; Reference proteome; Sensory transduction; Transcription;
KW Transcription regulation.
FT CHAIN 1..261
FT /note="Blue-light photoreceptor"
FT /id="PRO_0000172004"
FT DOMAIN 12..87
FT /note="PAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 88..138
FT /note="PAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT DOMAIN 147..258
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT MOD_RES 62
FT /note="S-4a-FMN cysteine"
FT HELIX 5..7
FT /evidence="ECO:0007829|PDB:4GCZ"
FT HELIX 9..20
FT /evidence="ECO:0007829|PDB:4GCZ"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:2PR5"
FT STRAND 39..42
FT /evidence="ECO:0007829|PDB:2PR5"
FT HELIX 44..50
FT /evidence="ECO:0007829|PDB:2PR5"
FT HELIX 54..57
FT /evidence="ECO:0007829|PDB:2PR5"
FT HELIX 62..65
FT /evidence="ECO:0007829|PDB:2PR5"
FT HELIX 72..84
FT /evidence="ECO:0007829|PDB:2PR5"
FT STRAND 88..95
FT /evidence="ECO:0007829|PDB:2PR5"
FT STRAND 101..113
FT /evidence="ECO:0007829|PDB:2PR5"
FT STRAND 116..125
FT /evidence="ECO:0007829|PDB:2PR5"
FT HELIX 127..145
FT /evidence="ECO:0007829|PDB:2PR5"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:2MWG"
FT HELIX 168..183
FT /evidence="ECO:0007829|PDB:2MWG"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:2MWG"
FT STRAND 188..193
FT /evidence="ECO:0007829|PDB:2MWG"
FT STRAND 195..198
FT /evidence="ECO:0007829|PDB:2MWG"
FT HELIX 202..218
FT /evidence="ECO:0007829|PDB:2MWG"
FT STRAND 221..226
FT /evidence="ECO:0007829|PDB:2MWG"
FT HELIX 229..238
FT /evidence="ECO:0007829|PDB:2MWG"
FT HELIX 241..244
FT /evidence="ECO:0007829|PDB:2MWG"
FT STRAND 247..249
FT /evidence="ECO:0007829|PDB:2MWG"
FT HELIX 251..257
FT /evidence="ECO:0007829|PDB:2MWG"
SQ SEQUENCE 261 AA; 29195 MW; 4AD6EB00B6BD27DD CRC64;
MASFQSFGIP GQLEVIKKAL DHVRVGVVIT DPALEDNPIV YVNQGFVQMT GYETEEILGK
NCRFLQGKHT DPAEVDNIRT ALQNKEPVTV QIQNYKKDGT MFWNELNIDP MEIEDKTYFV
GIQNDITKQK EYEKLLEDSL TEITALSTPI VPIRNGISAL PLVGNLTEER FNSIVCTLTN
ILSTSKDDYL IIDLSGLAQV NEQTADQIFK LSHLLKLTGT ELIITGIKPE LAMKMNKLDA
NFSSLKTYSN VKDAVKVLPI M
