ID   PHOU_ENTCL              Reviewed;         241 AA.
AC   O32488;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Phosphate-specific transport system accessory protein PhoU;
DE            Short=Pst system accessory protein PhoU;
GN   Name=phoU;
OS   Enterobacter cloacae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
OX   NCBI_TaxID=550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 7256 / NBRC 3320;
RX   PubMed=9324271; DOI=10.1128/jb.179.19.6192-6195.1997;
RA   Kusaka K., Shibata K., Kuroda A., Kato J., Ohtake H.;
RT   "Isolation and characterization of Enterobacter cloacae mutants which are
RT   defective in chemotaxis toward inorganic phosphate.";
RL   J. Bacteriol. 179:6192-6195(1997).
CC   -!- FUNCTION: Part of the phosphate (Pho) regulon, which plays a key role
CC       in phosphate homeostasis. Encoded together with proteins of the
CC       phosphate-specific transport (Pst) system in the polycistronic pstSCAB-
CC       phoU operon. PhoU is essential for the repression of the Pho regulon at
CC       high phosphate conditions. In this role, it may bind, possibly as a
CC       chaperone, to PhoR, PhoB or a PhoR-PhoB complex to promote
CC       dephosphorylation of phospho-PhoB, or inhibit formation of the PhoR-
CC       PhoB transitory complex (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PhoU family. {ECO:0000305}.
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DR   EMBL; D89963; BAA22865.1; -; Genomic_DNA.
DR   RefSeq; WP_008500182.1; NZ_WNXG01000150.1.
DR   AlphaFoldDB; O32488; -.
DR   SMR; O32488; -.
DR   STRING; 1399774.JDWH01000010_gene476; -.
DR   PRIDE; O32488; -.
DR   GeneID; 64726516; -.
DR   GeneID; 67464386; -.
DR   eggNOG; COG0704; Bacteria.
DR   OMA; WKHGIET; -.
DR   OrthoDB; 1648549at2; -.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0030643; P:cellular phosphate ion homeostasis; IEA:InterPro.
DR   GO; GO:0010629; P:negative regulation of gene expression; ISS:UniProtKB.
DR   GO; GO:0045936; P:negative regulation of phosphate metabolic process; ISS:UniProtKB.
DR   GO; GO:2000186; P:negative regulation of phosphate transmembrane transport; ISS:UniProtKB.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.58.220; -; 2.
DR   InterPro; IPR028366; P_transport_PhoU.
DR   InterPro; IPR038078; PhoU-like_sf.
DR   InterPro; IPR026022; PhoU_dom.
DR   PANTHER; PTHR42930; PTHR42930; 1.
DR   Pfam; PF01895; PhoU; 2.
DR   PIRSF; PIRSF003107; PhoU; 1.
DR   TIGRFAMs; TIGR02135; phoU_full; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Phosphate transport; Transport.
FT   CHAIN           1..241
FT                   /note="Phosphate-specific transport system accessory
FT                   protein PhoU"
FT                   /id="PRO_0000155169"
SQ   SEQUENCE   241 AA;  27467 MW;  09BB3C729A831AF7 CRC64;
     MDNLNLNKHI SGQFNAELES IRTQVMTMGG MVEQQLSDAI TAMHNQDSEL AKRVIEGDKN
     VNMMEVAIDE ACVRIIAKRQ PTASDLRLVM AIIKTIAELE RIGDVADKIC RTALEKFSQQ
     HQPLLVSLES LGRHTVQMLH DVLDAFARMD LDEAVRIYRE DKKVDQEYEG IVRQLMTYMM
     EDSRTIPSVL TALFCARSIE RIGDRCQNIC EYIFYFVKGQ DFRHVGGDEL DKLLAGKDPK
     E